研究样本浓度对浮游植物活体丰度估算值的影响

IF 2.1 4区 地球科学 Q2 MARINE & FRESHWATER BIOLOGY
Sean Yardley, Dawson Ogilvie, Oscar Casas-Monroy, Jocelyn Kydd, Robin Rozon, Sarah A. Bailey
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引用次数: 0

摘要

随着国际海事组织《国际压载水管理公约》于 2017 年 9 月生效,船舶已开始安装和运行船上压载水管理系统(BWMS),以减少压载水中的活生物体数量。为评估压载水管理系统在减少压载水中活生物体数量方面的效果,人们开发了科学方法。然而,考虑到分析 10-50 μm 大小生物的样本量较小(6 mL),检测经处理的压舱水中生物的低浓度具有挑战性。分析前可通过浓缩样品来增加分析量,但由于在浓缩步骤中可能会出现细胞损失,因此评估样品浓缩方法的效果非常重要。因此,我们进行了实验室实验,以评估重力过滤法将样本浓缩到 40:1 的效果。实验针对生物丰度低和生物丰度高两种情况进行。对于低生物丰度(10 个细胞 mL-1 以下)的未保存样本,在五次实验中的四次,浓缩样本比未浓缩样本平均少 31% 的活细胞 mL-1。在生物丰度较高(≥ 120 cells mL-1)的情况下,未保存的浓缩样本比未浓缩样本平均少 55% 的活细胞。相反,在生物丰度较低的情况下,保存的浓缩样本的细胞数平均比未浓缩样本多 4.5 倍。在生物丰度较高的情况下,浓缩样本的细胞数平均比未浓缩样本多 6.4 倍。保存样本和未保存样本之间也存在差异。这些发现有助于改进压舱水监测程序和 BWMS 评估,从而应对海洋环境保护面临的严峻挑战。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Examining effects of sample concentration on estimates of live phytoplankton abundance

With the entry into force of the International Maritime Organization's International Ballast Water Management Convention in September 2017, ships have begun to install and operate onboard ballast water management systems (BWMS) to reduce the number of live organisms in ballast water. Scientific methods were developed to assess the effectiveness of BWMS at reducing the number of live organisms in ballast water. However, detecting low organism concentrations in treated ballast water is challenging when considering the small sample volume (6 mL) analyzed for organisms in the 10–50 μm size class. The volume analyzed can be increased by concentrating the sample prior to analysis, but it is important to assess the effects of the sample concentration method due to potential cell loss experienced during the concentration step. Therefore, laboratory experiments were conducted to assess the effects of a gravity filtration method to concentrate samples to a factor of 40:1. Experiments were conducted for both low and high organism abundances. For unpreserved samples at low organism abundances (∼10 cells mL−1), concentrated samples had on average 31% fewer live cells mL−1 than unconcentrated samples for four out of five experiments. At high organism abundances (≥ 120 cells mL−1), unpreserved concentrated samples had on average 55% fewer live cells than unconcentrated samples. Alternatively, with preserved samples at low organism abundances, concentrated samples had on average 4.5× more cells than unconcentrated samples. At high organism abundances, concentrated samples had on average 6.4× more cells than unconcentrated samples. Differences were also observed between preserved and unpreserved samples. These findings can help to improve ballast water monitoring procedures and BWMS assessments, addressing a critical challenge to maritime environmental protection.

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来源期刊
Journal of Sea Research
Journal of Sea Research 地学-海洋学
CiteScore
3.20
自引率
5.00%
发文量
86
审稿时长
6-12 weeks
期刊介绍: The Journal of Sea Research is an international and multidisciplinary periodical on marine research, with an emphasis on the functioning of marine ecosystems in coastal and shelf seas, including intertidal, estuarine and brackish environments. As several subdisciplines add to this aim, manuscripts are welcome from the fields of marine biology, marine chemistry, marine sedimentology and physical oceanography, provided they add to the understanding of ecosystem processes.
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