鲴鱼(Ictalurus punctatus)的 TLR7 在溶酶体内表达,并受到合成 ssRNA 类似物、咪喹莫特和雷昔莫德的刺激。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Kristianna L. Felch , Jonathan D. Crider , Debduti Bhattacharjee , Cameron Huhn , Melanie Wilson , Eva Bengtén
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引用次数: 0

摘要

Toll 样受体(TLRs)是关键的模式识别受体(PRRs),也是先天性免疫的关键介质。尽管斑点叉尾鮰(Ictalurus punctatus)在比较免疫学和水产养殖业中具有重要意义,但其 20 个 TLR 基因在功能上仍基本未定性。在这项研究中,我们的目的是确定鲶鱼 TLR7 的激动剂、信号转导潜能和细胞定位。利用哺乳动物报告系统,我们确定了典型的 ssRNA 类似物 imiquimod 和 resiquimod,它们是有效的鲶鱼 TLR7 激动剂。值得注意的是,与草鱼 TLR7 不同,鲶鱼 TLR7 缺乏对 poly (I:C) 的反应能力。共聚焦显微镜显示,鲶鱼的 TLR7 主要在溶酶体中表达,并与内质体伴侣蛋白 UNC93B1 共定位。 此外,咪喹莫特刺激可在从成年鲶鱼分离出来的外周血白细胞中引起强有力的 IFNb 转录。这些发现强调了 TLR7 信号在鲶鱼中的保留,与哺乳动物的 TLR7 反应相似。我们的研究揭示了鲶鱼 TLR7 的功能方面,有助于更好地理解其在免疫防御机制中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
TLR7 in channel catfish (Ictalurus punctatus) is expressed in the endolysosome and is stimulated by synthetic ssRNA analogs, imiquimod, and resiquimod.

Toll-like receptors (TLRs) are pivotal pattern recognition receptors (PRRs) and key mediators of innate immunity. Despite the significance of channel catfish (Ictalurus punctatus) in comparative immunology and aquaculture, its 20 TLR genes remain largely functionally uncharacterized. In this study, our aim was to determine the catfish TLR7 agonists, signaling potential, and cellular localization. Using a mammalian reporter system, we identified imiquimod and resiquimod, typical ssRNA analogs, as potent catfish TLR7 agonists. Notably, unlike grass carp TLR7, catfish TLR7 lacks the ability to respond to poly (I:C). Confocal microscopy revealed predominant catfish TLR7 expression in lysosomes, co-localizing with the endosomal chaperone protein, UNC93B1. Furthermore, imiquimod stimulation elicited robust IFNb transcription in peripheral blood leukocytes isolated from adult catfish. These findings underscore the conservation of TLR7 signaling in catfish, reminiscent of mammalian TLR7 responses. Our study sheds light on the functional aspects of catfish TLR7 and contributes to a better understanding of its role in immune defense mechanisms.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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