评估 HHV-8 病毒载量的两种商业诊断方法

IF 1.5 Q4 INFECTIOUS DISEASES
Honorine Fenaux , Lina Mouna , Corinne Vieux-Combe , Isabelle Thouard , Philippe Colliot , Anne-Marie Roque-Afonso
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引用次数: 0

摘要

目的人类疱疹病毒-8(HHV-8)可导致卡波西肉瘤或 B 淋巴增生性疾病,如多中心 Castleman 病。患者随访以评估 HHV-8 病毒载量为基础,通常采用实时聚合酶链反应 (PCR) 来实现。一些实验室过去曾使用过 HHV-8 Premix r 基因试剂盒(生物梅里埃公司),但生物梅里埃公司已于 2021-2022 年停止生产和销售该试剂盒。其他试剂盒也需要经过测试才能用于诊断目的。在此,我们对两种商业试剂盒进行了评估:HHV8 ELITe MGB试剂盒(ELITech)和Quanty HHV-8(Clonit),并与HHV-8 Premix r-基因试剂盒进行了比较。总共有 46 份样本(37 份 HHV-8 阳性,9 份 HHV-8 阴性)使用 ELITe MGB 试剂盒进行了检测,37 份样本(29 份 HHV-8 阳性,8 份 HHV-8 阴性)使用 Quanty HHV-8 试剂盒进行了检测。使用 Analyse-it 软件对不同方法进行了 Bland-Altman 和 Passing-Bablok 检验比较。结果除了一个 HHV-8 低阳性样本被 ELITe MGB 套件检测为阴性外,其他定量结果一致。定量结果也一致;与 Premix r-gene 试剂盒相比,两种试剂盒的平均差异分别为 0.58 log10 copies/ml 和 0.73 log10 copies/ml。需要提醒的是,HHV-8 的定量检测目前还没有国际标准,因此应始终使用相同的方法对患者进行跟踪检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of two commercial diagnostic methods for HHV-8 viral load assessment

Objectives

Human herpesvirus-8 (HHV-8) can cause Kaposi's sarcoma or B lymphoproliferative disorders such as multicentric Castleman disease. Patient follow-up is based on assessing the HHV-8 viral load, which is usually achieved using real-time polymerase chain reaction (PCR). The HHV-8 Premix r-gene kit (BioMérieux) was used by some laboratories in the past, but BioMérieux ceased the production and distribution of this kit in 2021-2022. Other kits need to be tested so that they can be used for diagnostic purposes. Here we evaluated two commercial kits: HHV8 ELITe MGB Kit (ELITech) and Quanty HHV-8 (Clonit) and compared them with the HHV-8 Premix r-gene kit.

Methods

We used whole blood samples that had previously been tested with the HHV-8 Premix r-gene kit for diagnostic purposes. Overall, 46 samples (37 HHV-8-positive and 9 HHV-8-negative) were tested with the ELITe MGB Kit and 37 (29 HHV-8-positive and 8 HHV-8-negative) with the Quanty HHV-8 kit. The different methods were compared using Bland-Altman and Passing-Bablok tests with Analyse-it software.

Results

Qualitative results were concordant except for one HHV-8 low-positive sample that was found to be negative by the ELITe MGB Kit. The quantitative results were also concordant; both kits showed mean differences of 0.58 log10 copies/ml and 0.73 log10 copies/ml, respectively, compared to the Premix r-gene kit.

Conclusions

Both the methods tested produced acceptable results and could be used for diagnostic purposes. It should be remembered that there is no international standard for HHV-8 quantification and that patients should always be followed using the same method.

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来源期刊
IJID regions
IJID regions Infectious Diseases
CiteScore
1.60
自引率
0.00%
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64 days
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