Ag+ 与双链 DNA 中的中心 C-C 错配碱基对而非末端 C-C 对的特异性结合

IF 3.1 2区 化学 Q2 CHEMISTRY, ANALYTICAL
Hidetaka Torigoe, Hayahide Kida
{"title":"Ag+ 与双链 DNA 中的中心 C-C 错配碱基对而非末端 C-C 对的特异性结合","authors":"Hidetaka Torigoe,&nbsp;Hayahide Kida","doi":"10.1016/j.tca.2024.179770","DOIUrl":null,"url":null,"abstract":"<div><p>Metal ion-nucleic acid interactions are important for their contribution in structure formation and their potential applications in nanotechnology. Hg<sup>2+</sup> and Ag<sup>+</sup> bind to T–T and C<img>C mismatched base pairs, respectively, at the center of duplex DNA to form T–Hg–T and C–Ag–C. Although primer-extension by DNA polymerases with Hg<sup>2+</sup> incorporated thymidine 5′-triphosphate to form T–Hg–T, the same reaction with Ag<sup>+</sup> did not incorporate deoxycytidine 5′-triphosphate to form C–Ag–C. Here, isothermal titration calorimetric analyses to examine the effect of C<img>C position in duplex DNA on Ag<sup>+</sup> binding demonstrated that Ag<sup>+</sup> did not bind to the terminal C<img>C base pair in duplex, but it bound to the central C<img>C base pair in duplex at 1:1 molar ratio with 9 × 10<sup>5</sup> M<sup>–1</sup> binding constant. Ag<sup>+</sup> did not bind to the terminal and central C–A, C–G, and C–T base pairs in duplex. These findings are useful for developing efficient metal-mediated base pair formation in nanotechnology.</p></div>","PeriodicalId":23058,"journal":{"name":"Thermochimica Acta","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Specific binding of Ag+ to central CC mismatched base pair but not terminal CC pair in duplex DNA\",\"authors\":\"Hidetaka Torigoe,&nbsp;Hayahide Kida\",\"doi\":\"10.1016/j.tca.2024.179770\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Metal ion-nucleic acid interactions are important for their contribution in structure formation and their potential applications in nanotechnology. Hg<sup>2+</sup> and Ag<sup>+</sup> bind to T–T and C<img>C mismatched base pairs, respectively, at the center of duplex DNA to form T–Hg–T and C–Ag–C. Although primer-extension by DNA polymerases with Hg<sup>2+</sup> incorporated thymidine 5′-triphosphate to form T–Hg–T, the same reaction with Ag<sup>+</sup> did not incorporate deoxycytidine 5′-triphosphate to form C–Ag–C. Here, isothermal titration calorimetric analyses to examine the effect of C<img>C position in duplex DNA on Ag<sup>+</sup> binding demonstrated that Ag<sup>+</sup> did not bind to the terminal C<img>C base pair in duplex, but it bound to the central C<img>C base pair in duplex at 1:1 molar ratio with 9 × 10<sup>5</sup> M<sup>–1</sup> binding constant. Ag<sup>+</sup> did not bind to the terminal and central C–A, C–G, and C–T base pairs in duplex. These findings are useful for developing efficient metal-mediated base pair formation in nanotechnology.</p></div>\",\"PeriodicalId\":23058,\"journal\":{\"name\":\"Thermochimica Acta\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2024-05-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Thermochimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0040603124001096\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Thermochimica Acta","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0040603124001096","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

摘要

金属离子与核酸的相互作用对结构的形成及其在纳米技术中的潜在应用具有重要意义。Hg2+ 和 Ag+ 分别与双链 DNA 中心的 T-T 和 CC 错配碱基对结合,形成 T-Hg-T 和 C-Ag-C。虽然 DNA 聚合酶用 Hg2+ 进行引物延伸时会结合胸苷 5′-三磷酸形成 T-Hg-T,但用 Ag+ 进行同样的反应时不会结合脱氧胞苷 5′-三磷酸形成 C-Ag-C。等温滴定量热分析表明,Ag+不与双链中末端的CC碱基对结合,但与双链中中心的CC碱基对结合,摩尔比为1:1,结合常数为9×105 M-1。Ag+ 不与双链中的末端和中心 C-A、C-G 和 C-T 碱基对结合。这些发现有助于在纳米技术中开发高效的金属介导的碱基对形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Specific binding of Ag+ to central CC mismatched base pair but not terminal CC pair in duplex DNA

Specific binding of Ag+ to central CC mismatched base pair but not terminal CC pair in duplex DNA

Metal ion-nucleic acid interactions are important for their contribution in structure formation and their potential applications in nanotechnology. Hg2+ and Ag+ bind to T–T and CC mismatched base pairs, respectively, at the center of duplex DNA to form T–Hg–T and C–Ag–C. Although primer-extension by DNA polymerases with Hg2+ incorporated thymidine 5′-triphosphate to form T–Hg–T, the same reaction with Ag+ did not incorporate deoxycytidine 5′-triphosphate to form C–Ag–C. Here, isothermal titration calorimetric analyses to examine the effect of CC position in duplex DNA on Ag+ binding demonstrated that Ag+ did not bind to the terminal CC base pair in duplex, but it bound to the central CC base pair in duplex at 1:1 molar ratio with 9 × 105 M–1 binding constant. Ag+ did not bind to the terminal and central C–A, C–G, and C–T base pairs in duplex. These findings are useful for developing efficient metal-mediated base pair formation in nanotechnology.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Thermochimica Acta
Thermochimica Acta 化学-分析化学
CiteScore
6.50
自引率
8.60%
发文量
210
审稿时长
40 days
期刊介绍: Thermochimica Acta publishes original research contributions covering all aspects of thermoanalytical and calorimetric methods and their application to experimental chemistry, physics, biology and engineering. The journal aims to span the whole range from fundamental research to practical application. The journal focuses on the research that advances physical and analytical science of thermal phenomena. Therefore, the manuscripts are expected to provide important insights into the thermal phenomena studied or to propose significant improvements of analytical or computational techniques employed in thermal studies. Manuscripts that report the results of routine thermal measurements are not suitable for publication in Thermochimica Acta. The journal particularly welcomes papers from newly emerging areas as well as from the traditional strength areas: - New and improved instrumentation and methods - Thermal properties and behavior of materials - Kinetics of thermally stimulated processes
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信