Kumars Jovaini , Seyed Amir Mohammad Mortazavian Farsani , Seyed Hamid Aghaee-Bakhtiari , Sahar Baniyaghoob
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Subsequently, the cytotoxicity of the optimized ratio of chitosan/alginate nanoparticles was investigated on MCF-7 cancer cells.</p></div><div><h3>Results</h3><p>The research revealed that optimal conditions for the mentioned variables were a chitosan concentration of 1% w/w, an alginate concentration of 1% w/w, and a <em>L. bulgaricus</em> count of 8.15 CFU/ml. Following numerical optimization, the loading efficacy = 91.15%, the release = 71.55%, the polydispersity index = 0.11, and the ζ-potential = 61.94 based on numerical optimization. Findings revealed that miracle drinks with <em>L. bulgaricus-loaded</em> chitosan/alginate microcapsule ratios exhibited toxic and potential apoptotic effects on MCF-7 cancer cells. This study showed that a miracle drink prepared with the optimal ratio of probiotic nanoparticles stops cells in the S and G2/M phases.</p></div><div><h3>Conclusions</h3><p>The results show that Miracle drink supplemented with <em>L. bulgaricus</em> loaded-chitosan/alginate nanoparticles has a toxic and lethal effect on MCF-7 cancer cells. This compound can be suggested and used as an alternative candidate or complementary cancer therapy.</p><p><strong>How to cite:</strong> Jovaini K, Mortazavian Farsani SAM, Aghaee-Bakhtiari SH, et al. Miracle drink supplemented with <em>L.bulgaricus</em> loaded-chitosan/alginate nanoparticles as a medicinal food for control of MCF7 cancer cells. Electron J Biotechnol 2024;69. <span>https://doi.org/10.1016/j.ejbt.2024.04.002</span><svg><path></path></svg>.</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"70 ","pages":"Pages 1-13"},"PeriodicalIF":2.3000,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345824000101/pdfft?md5=b91383e68bbca1f5ca9be40e85842d5b&pid=1-s2.0-S0717345824000101-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Miracle drink supplemented with Lactobacillus bulgaricus loaded-chitosan/alginate nanoparticles as a medicinal food for control of MCF7 cancer cells\",\"authors\":\"Kumars Jovaini , Seyed Amir Mohammad Mortazavian Farsani , Seyed Hamid Aghaee-Bakhtiari , Sahar Baniyaghoob\",\"doi\":\"10.1016/j.ejbt.2024.04.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Cancer continues to be one of the greatest challenges in modern medicine and is second only to cardiovascular disease as the main cause of death. Breast cancer in particular is responsible for 15% of deaths in women. In this study, <em>Lactobacillus bulgaricus</em> was microencapsulated using a chitosan/alginate mixture. Parameters such as chitosan, alginate, and <em>L. bulgaricus</em> populations were optimized using Design Expert software. The responses were loading efficiency, particle size, release, and ζ-potential. Subsequently, the cytotoxicity of the optimized ratio of chitosan/alginate nanoparticles was investigated on MCF-7 cancer cells.</p></div><div><h3>Results</h3><p>The research revealed that optimal conditions for the mentioned variables were a chitosan concentration of 1% w/w, an alginate concentration of 1% w/w, and a <em>L. bulgaricus</em> count of 8.15 CFU/ml. Following numerical optimization, the loading efficacy = 91.15%, the release = 71.55%, the polydispersity index = 0.11, and the ζ-potential = 61.94 based on numerical optimization. Findings revealed that miracle drinks with <em>L. bulgaricus-loaded</em> chitosan/alginate microcapsule ratios exhibited toxic and potential apoptotic effects on MCF-7 cancer cells. This study showed that a miracle drink prepared with the optimal ratio of probiotic nanoparticles stops cells in the S and G2/M phases.</p></div><div><h3>Conclusions</h3><p>The results show that Miracle drink supplemented with <em>L. bulgaricus</em> loaded-chitosan/alginate nanoparticles has a toxic and lethal effect on MCF-7 cancer cells. This compound can be suggested and used as an alternative candidate or complementary cancer therapy.</p><p><strong>How to cite:</strong> Jovaini K, Mortazavian Farsani SAM, Aghaee-Bakhtiari SH, et al. Miracle drink supplemented with <em>L.bulgaricus</em> loaded-chitosan/alginate nanoparticles as a medicinal food for control of MCF7 cancer cells. 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引用次数: 0
摘要
背景癌症仍然是现代医学面临的最大挑战之一，是仅次于心血管疾病的第二大死亡原因。尤其是乳腺癌，占女性死亡原因的 15%。本研究使用壳聚糖/海藻酸盐混合物对保加利亚乳杆菌进行微囊化。使用 Design Expert 软件对壳聚糖、海藻酸盐和保加利亚乳杆菌数量等参数进行了优化。反应为装载效率、粒度、释放和ζ电位。结果研究表明，上述变量的最佳条件是壳聚糖浓度为 1%（湿重），海藻酸浓度为 1%（湿重），保加利亚酵母菌数量为 8.15 CFU/ml。经过数值优化，负载功效 = 91.15%，释放 = 71.55%，多分散指数 = 0.11，ζ-电位 = 61.94。研究结果表明，壳聚糖/海藻酸微胶囊配比的保加利亚乳杆菌神奇饮料对 MCF-7 癌细胞具有毒性和潜在的凋亡作用。研究结果表明，以最佳益生菌纳米粒子比例制备的神奇饮料能使细胞停止在 S 期和 G2/M 期。该化合物可作为癌症治疗的替代选择或补充疗法：Jovaini K, Mortazavian Farsani SAM, Aghaee-Bakhtiari SH, et al. Miracle drink supplemented with L.bulgaricus loaded-chitosan/alginate nanoparticles as a medicinal food for control of MCF7 cancer cells.Electron J Biotechnol 2024;69. https://doi.org/10.1016/j.ejbt.2024.04.002.
Miracle drink supplemented with Lactobacillus bulgaricus loaded-chitosan/alginate nanoparticles as a medicinal food for control of MCF7 cancer cells
Background
Cancer continues to be one of the greatest challenges in modern medicine and is second only to cardiovascular disease as the main cause of death. Breast cancer in particular is responsible for 15% of deaths in women. In this study, Lactobacillus bulgaricus was microencapsulated using a chitosan/alginate mixture. Parameters such as chitosan, alginate, and L. bulgaricus populations were optimized using Design Expert software. The responses were loading efficiency, particle size, release, and ζ-potential. Subsequently, the cytotoxicity of the optimized ratio of chitosan/alginate nanoparticles was investigated on MCF-7 cancer cells.
Results
The research revealed that optimal conditions for the mentioned variables were a chitosan concentration of 1% w/w, an alginate concentration of 1% w/w, and a L. bulgaricus count of 8.15 CFU/ml. Following numerical optimization, the loading efficacy = 91.15%, the release = 71.55%, the polydispersity index = 0.11, and the ζ-potential = 61.94 based on numerical optimization. Findings revealed that miracle drinks with L. bulgaricus-loaded chitosan/alginate microcapsule ratios exhibited toxic and potential apoptotic effects on MCF-7 cancer cells. This study showed that a miracle drink prepared with the optimal ratio of probiotic nanoparticles stops cells in the S and G2/M phases.
Conclusions
The results show that Miracle drink supplemented with L. bulgaricus loaded-chitosan/alginate nanoparticles has a toxic and lethal effect on MCF-7 cancer cells. This compound can be suggested and used as an alternative candidate or complementary cancer therapy.
How to cite: Jovaini K, Mortazavian Farsani SAM, Aghaee-Bakhtiari SH, et al. Miracle drink supplemented with L.bulgaricus loaded-chitosan/alginate nanoparticles as a medicinal food for control of MCF7 cancer cells. Electron J Biotechnol 2024;69. https://doi.org/10.1016/j.ejbt.2024.04.002.
期刊介绍：
Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology.
The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th).
The following areas are covered in the Journal:
• Animal Biotechnology
• Biofilms
• Bioinformatics
• Biomedicine
• Biopolicies of International Cooperation
• Biosafety
• Biotechnology Industry
• Biotechnology of Human Disorders
• Chemical Engineering
• Environmental Biotechnology
• Food Biotechnology
• Marine Biotechnology
• Microbial Biotechnology
• Molecular Biology and Genetics
•Nanobiotechnology
• Omics
• Plant Biotechnology
• Process Biotechnology
• Process Chemistry and Technology
• Tissue Engineering
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