三七浆果中主要水溶性酸性多糖(AGBP-A3)的结构分析和抗炎活性

IF 6.8 2区 医学 Q1 CHEMISTRY, MEDICINAL
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引用次数: 0

摘要

背景五加科植物板蓝根(Panax quinquefolius L)因其对医学的宝贵贡献而广受认可,在全球范围内引起了极大的关注。与人们对五加科植物根的广泛研究不同,人们对其浆果的关注相对较少。材料和方法用热水提取五倍子浆果,用酒精沉淀,用 DEAE-52 纤维素柱分离,得到一系列馏分。其中一个馏分经 Sephadex G-200 柱进一步纯化,得到三个馏分。然后,通过甲基化分析、核磁共振光谱等方法对名为 AGBP-A3 的主要馏分进行表征。结果 主链由α-L-Rhap、α-D-GalAp和β-D-Galp组成,支链主要由α-L-Araf、β-D-Glcp、α-D-GalAp和β-D-Galp组成。RAW264.7 细胞试验结果表明,浓度为 625 ng/mL 时,对 IL-6 和 IL-1β 分泌的抑制率分别为 24.83 % 和 11.84 %;浓度为 312 ng/mL 时,对 IL-10 分泌的抑制率为 70.17 %。在斑马鱼试验中,迁移的中性粒细胞数量明显减少,其向炎症组织的迁移也受到抑制。本研究证明了五倍子浆果酸性多糖的结构及其对炎症的影响,为筛选抗炎药物提供了参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Structural analysis, anti-inflammatory activity of the main water-soluble acidic polysaccharides (AGBP-A3) from Panax quinquefolius L berry

Structural analysis, anti-inflammatory activity of the main water-soluble acidic polysaccharides (AGBP-A3) from Panax quinquefolius L berry

Structural analysis, anti-inflammatory activity of the main water-soluble acidic polysaccharides (AGBP-A3) from Panax quinquefolius L berry

Background

Panax quinquefolius L, widely recognized for its valuable contributions to medicine, has aroused considerable attention globally. Different from the extensive research has been dedicated to the root of P. quinquefolius, its berry has received relatively scant focus. Given its promising medicinal properties, this study was focused on the structural characterizations and anti-inflammatory potential of acidic polysaccharides from the P. quinquefolius berry.

Materials and methods

P. quinquefolius berry was extracted with hot water, precipitated by alcohol, separated by DEAE-52-cellulose column to give a series of fractions. One of these fractions was further purified via Sephadex G-200 column to give three fractions. Then, the main fraction named as AGBP-A3 was characterized by methylation analysis, NMR spectroscopy, etc. Its anti-inflammatory activity was assessed by RAW 264.7 cell model, zebrafish model and molecular docking.

Results

The main chain comprised of α-L-Rhap, α-D-GalAp and β-D-Galp, while the branch consisted mainly of α-L-Araf, β-D-Glcp, α-D-GalAp, β-D-Galp. The RAW264.7 cell assay results showed that the inhibition rates against IL-6 and IL-1β secretion at the concentration of 625 ng/mL were 24.83 %, 11.84 %, while the inhibition rate against IL-10 secretion was 70.17 % at the concentration of 312 ng/mL. In the zebrafish assay, the migrating neutrophils were significantly reduced in number, and their migration to inflammatory tissues was inhibited. Molecular docking predictions correlated well with the results of the anti-inflammatory assay.

Conclusion

The present study demonstrated the structure of acidic polysaccharides of P. quinquefolius berry and their effect on inflammation, providing a reference for screening anti-inflammatory drugs.

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来源期刊
Journal of Ginseng Research
Journal of Ginseng Research CHEMISTRY, MEDICINAL-INTEGRATIVE & COMPLEMENTARY MEDICINE
CiteScore
11.40
自引率
9.50%
发文量
111
审稿时长
6-12 weeks
期刊介绍: Journal of Ginseng Research (JGR) is an official, open access journal of the Korean Society of Ginseng and is the only international journal publishing scholarly reports on ginseng research in the world. The journal is a bimonthly peer-reviewed publication featuring high-quality studies related to basic, pre-clinical, and clinical researches on ginseng to reflect recent progresses in ginseng research. JGR publishes papers, either experimental or theoretical, that advance our understanding of ginseng science, including plant sciences, biology, chemistry, pharmacology, toxicology, pharmacokinetics, veterinary medicine, biochemistry, manufacture, and clinical study of ginseng since 1976. It also includes the new paradigm of integrative research, covering alternative medicinal approaches. Article types considered for publication include review articles, original research articles, and brief reports. JGR helps researchers to understand mechanisms for traditional efficacy of ginseng and to put their clinical evidence together. It provides balanced information on basic science and clinical applications to researchers, manufacturers, practitioners, teachers, scholars, and medical doctors.
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