Nazma Khan , Mohammad Khushtar , Md Azizur Rahman , Md Kaish , Mohd Ajmal
{"title":"通过预防粘液氧化应激，使用莽草水醇提取物改善 Sprague Dawley 大鼠的胃溃疡状况","authors":"Nazma Khan , Mohammad Khushtar , Md Azizur Rahman , Md Kaish , Mohd Ajmal","doi":"10.1016/j.prmcm.2024.100442","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Gastric ulcer is a significant global public health concern that can result in severe complications such as gastro-intestinal bleeding, perforations, gastro-intestinal obstruction and cancer. Mangifera indica seed kernel, a traditional Chinese medicine, is widely available and used in China. The current study was aimed to determine gastro-protective effect of Mangifera indica kernel extract in preventing gastric ulcer in rats.</p></div><div><h3>Methods</h3><p>SD rats were divided into six groups. Each group consisted of five rats. Group-I received 1 ml/kg/day p.o. of 1 % carboxy methylcellulose. Group-II received indomethacin (20 mg/kg/day, p.o.). Group-III received Mangifera indica kernel extract (500 mg/kg/day, p.o.). Group-IV received the extract (1000 mg/kg/day, p.o.). Group-V received 50 mg/kg/day ranitidine. Group-VI (per se) received the extract (1000 mg/kg/day, p.o.). The ulcer index, mucus barrier, and histopathology were calculated. After homogenization of gastric tissue in buffer, anti-oxidant enzyme potency was also evaluated.</p></div><div><h3>Results</h3><p>Mangifera indica kernel extract significantly (<em>p</em> < 0.01) and dose-dependently increased mucus, superoxide dismutase, and catalase levels while lowered ulcer index and thiobarbituric acid reactive substances in comparison to ulcer control group-II. According to histopathological data, indomethacin medication led to gastric ulcers whereas Mangifera indica kernel extract protected from gastric ulcer by prevention of indomethacin-induced muco-oxidative stress. Mangifera indica 1000 mg/kg/day in group-IV had ulcer prevention potency comparable to ranitidine 50 mg/kg/day in group-V.</p></div><div><h3>Discussion</h3><p>Thus, Mangifera indica extract possesses promising effect on amelioration of gastric ulcer and protects from gastric ulcer by prevention of indomethacin-induced muco-oxidative stress, re-inforcing the gastric mucosa, and reducing the secretion of gastric acid.</p></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"11 ","pages":"Article 100442"},"PeriodicalIF":0.0000,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266714252400085X/pdfft?md5=9519b3e2b8bdcc4133f8a006c4516848&pid=1-s2.0-S266714252400085X-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Amelioration of gastric ulcer using a hydro-alcoholic extract of Mangifera indica in Sprague Dawley rats by prevention of muco-oxidative stress\",\"authors\":\"Nazma Khan , Mohammad Khushtar , Md Azizur Rahman , Md Kaish , Mohd Ajmal\",\"doi\":\"10.1016/j.prmcm.2024.100442\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Gastric ulcer is a significant global public health concern that can result in severe complications such as gastro-intestinal bleeding, perforations, gastro-intestinal obstruction and cancer. Mangifera indica seed kernel, a traditional Chinese medicine, is widely available and used in China. The current study was aimed to determine gastro-protective effect of Mangifera indica kernel extract in preventing gastric ulcer in rats.</p></div><div><h3>Methods</h3><p>SD rats were divided into six groups. Each group consisted of five rats. Group-I received 1 ml/kg/day p.o. of 1 % carboxy methylcellulose. Group-II received indomethacin (20 mg/kg/day, p.o.). Group-III received Mangifera indica kernel extract (500 mg/kg/day, p.o.). Group-IV received the extract (1000 mg/kg/day, p.o.). Group-V received 50 mg/kg/day ranitidine. Group-VI (per se) received the extract (1000 mg/kg/day, p.o.). The ulcer index, mucus barrier, and histopathology were calculated. After homogenization of gastric tissue in buffer, anti-oxidant enzyme potency was also evaluated.</p></div><div><h3>Results</h3><p>Mangifera indica kernel extract significantly (<em>p</em> < 0.01) and dose-dependently increased mucus, superoxide dismutase, and catalase levels while lowered ulcer index and thiobarbituric acid reactive substances in comparison to ulcer control group-II. According to histopathological data, indomethacin medication led to gastric ulcers whereas Mangifera indica kernel extract protected from gastric ulcer by prevention of indomethacin-induced muco-oxidative stress. Mangifera indica 1000 mg/kg/day in group-IV had ulcer prevention potency comparable to ranitidine 50 mg/kg/day in group-V.</p></div><div><h3>Discussion</h3><p>Thus, Mangifera indica extract possesses promising effect on amelioration of gastric ulcer and protects from gastric ulcer by prevention of indomethacin-induced muco-oxidative stress, re-inforcing the gastric mucosa, and reducing the secretion of gastric acid.</p></div>\",\"PeriodicalId\":101013,\"journal\":{\"name\":\"Pharmacological Research - Modern Chinese Medicine\",\"volume\":\"11 \",\"pages\":\"Article 100442\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-05-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S266714252400085X/pdfft?md5=9519b3e2b8bdcc4133f8a006c4516848&pid=1-s2.0-S266714252400085X-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pharmacological Research - Modern Chinese Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S266714252400085X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological Research - Modern Chinese Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S266714252400085X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Amelioration of gastric ulcer using a hydro-alcoholic extract of Mangifera indica in Sprague Dawley rats by prevention of muco-oxidative stress
Background
Gastric ulcer is a significant global public health concern that can result in severe complications such as gastro-intestinal bleeding, perforations, gastro-intestinal obstruction and cancer. Mangifera indica seed kernel, a traditional Chinese medicine, is widely available and used in China. The current study was aimed to determine gastro-protective effect of Mangifera indica kernel extract in preventing gastric ulcer in rats.
Methods
SD rats were divided into six groups. Each group consisted of five rats. Group-I received 1 ml/kg/day p.o. of 1 % carboxy methylcellulose. Group-II received indomethacin (20 mg/kg/day, p.o.). Group-III received Mangifera indica kernel extract (500 mg/kg/day, p.o.). Group-IV received the extract (1000 mg/kg/day, p.o.). Group-V received 50 mg/kg/day ranitidine. Group-VI (per se) received the extract (1000 mg/kg/day, p.o.). The ulcer index, mucus barrier, and histopathology were calculated. After homogenization of gastric tissue in buffer, anti-oxidant enzyme potency was also evaluated.
Results
Mangifera indica kernel extract significantly (p < 0.01) and dose-dependently increased mucus, superoxide dismutase, and catalase levels while lowered ulcer index and thiobarbituric acid reactive substances in comparison to ulcer control group-II. According to histopathological data, indomethacin medication led to gastric ulcers whereas Mangifera indica kernel extract protected from gastric ulcer by prevention of indomethacin-induced muco-oxidative stress. Mangifera indica 1000 mg/kg/day in group-IV had ulcer prevention potency comparable to ranitidine 50 mg/kg/day in group-V.
Discussion
Thus, Mangifera indica extract possesses promising effect on amelioration of gastric ulcer and protects from gastric ulcer by prevention of indomethacin-induced muco-oxidative stress, re-inforcing the gastric mucosa, and reducing the secretion of gastric acid.
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