头足类水螅中的 FMRFamide Na+ 通道受体:鉴定、表征和性腺发育不同阶段的表达谱分析

IF 2.5 3区 医学 Q3 ENDOCRINOLOGY & METABOLISM
Hui-Min Cao , Jun-Hong Wu , Shuang Li, Xu Zhou, Li-Bing Zheng, Chang-Feng Chi
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引用次数: 0

摘要

神经肽家族成员之一的 FMRFamide 参与了许多生理过程。FMRFamide激活的钠通道(FaNaCs)是由神经肽FMRFamide触发的非电压门控、氨苯蝶啶敏感、Na+选择性通道家族。本研究克隆了日本水蚤 FaNaC 受体(SjFaNaC)的全长 cDNA。SjFaNaC 的 cDNA 长 3004 bp,开放阅读框(ORF)长 1812 bp,编码 603 个氨基酸残基,N 端无信号肽。序列分析表明,SjFaNaC 与其他头足类动物的 FaNaC 具有很高的同一性,并与双壳类动物形成姊妹支系。以 AcFaNaC 为模板,使用 SWISS-MODEL 预测了蛋白质结构。定量实时 PCR(qRT-PCR)显示,SjFaNaC 转录本在雌性和雄性生殖器官以及中枢神经系统(CNS)的视叶和大脑中均有高表达。原位杂交(ISH)结果显示,SjFaNaC mRNA主要分布在视叶的髓质和深视网膜以及大脑的食管上和食管下肿块。亚细胞定位表明,SjFaNaC 蛋白定位于 HEK293T 细胞的细胞内和细胞表面。总之,这些发现可能为今后探索 SjFaNaC 在头足类动物中的功能奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Na+ channel receptor of FMRFamide in the cephalopod Sepiella japonica: Identification, characterisation, and expression profiling during different stages of gonadal development

FMRFamide, a member of the neuropeptide family, is involved in numerous physiological processes. FMRFamide-activated sodium channels (FaNaCs) are a family of non-voltage-gated, amiloride-sensitive, Na+-selective channels triggered by the neuropeptide FMRFamide. In the present study, the full-length cDNA of the FaNaC receptor of Sepiella japonica (SjFaNaC) was cloned. The cDNA of SjFaNaC was 3004 bp long with an open reading frame (ORF) of 1812 bp, encoding 603 amino acid residues with no signal peptide at the N-terminus. Sequence analysis indicated that SjFaNaC shared a high identity with other cephalopods FaNaCs and formed a sister clade with bivalves. The protein structure was predicted using SWISS-MODEL with AcFaNaC as the template. Quantitative real-time PCR (qRT-PCR) revealed that SjFaNaC transcripts were highly expressed in both female and male reproductive organs, as well as in the optic lobe and brain of the central nervous system (CNS). Results of in situ hybridisation (ISH) showed that SjFaNaC mRNA was mainly distributed in the medulla and deep retina of the optic lobe and in both the supraesophageal and subesophageal masses of the brain. Subcellular localisation indicated that the SjFaNaC protein was localised intracellularly and on the cell surface of HEK293T cells. In summary, these findings may lay the foundation for future exploration of the functions of SjFaNaC in cephalopods.

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来源期刊
Neuropeptides
Neuropeptides 医学-内分泌学与代谢
CiteScore
5.40
自引率
6.90%
发文量
55
审稿时长
>12 weeks
期刊介绍: The aim of Neuropeptides is the rapid publication of original research and review articles, dealing with the structure, distribution, actions and functions of peptides in the central and peripheral nervous systems. The explosion of research activity in this field has led to the identification of numerous naturally occurring endogenous peptides which act as neurotransmitters, neuromodulators, or trophic factors, to mediate nervous system functions. Increasing numbers of non-peptide ligands of neuropeptide receptors have been developed, which act as agonists or antagonists in peptidergic systems. The journal provides a unique opportunity of integrating the many disciplines involved in all neuropeptide research. The journal publishes articles on all aspects of the neuropeptide field, with particular emphasis on gene regulation of peptide expression, peptide receptor subtypes, transgenic and knockout mice with mutations in genes for neuropeptides and peptide receptors, neuroanatomy, physiology, behaviour, neurotrophic factors, preclinical drug evaluation, clinical studies, and clinical trials.
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