基于扩增子的元基因组学研究椰糠龄期和木质生物炭对草莓产量相关微生物组的影响

IF 3.5 Q1 AGRONOMY
Xiangming Xu, G. Deakin, Jingchen Zhao, T. Passey, Matevz Papp-Rupar
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引用次数: 0

摘要

在英国,草莓大多在椰糠基质的保护下生长。椰糠基质通常只用于一到两个种植季节,因为连续重复使用椰糠而不进行任何处理会导致产量下降。在这项研究中,我们调查了草莓根部和散装椰糠中细菌和真菌群落的变化与以下因素的关系:(i) 椰糠基质的年龄(种植季节);(ii) 种植时橡树或榉树生物炭的添加剂。棕壤年龄不影响真菌/细菌的α(样本内)多样性,但影响β(样本间)多样性。添加橡木或榉木生物炭不会导致真菌和细菌的阿尔法或贝塔多样性发生显著变化,但会改变 13 种真菌 ASV 的相对丰度。这项研究发现,有 6 种细菌和 20 种真菌 ASV 与椰糠龄期呈显著的正线性关系,还有 8 种细菌和 22 种真菌 ASV 与椰糠龄期呈显著的负线性关系。值得注意的是,在重复使用的椰糠基质中观察到的草莓产量下降可能与一种通用根部病原体 Ilyonectria destructans(前 Cylindrocarpon destructans)有关,这种病原体在草莓根部和散装椰糠中的数量每年分别增加 225% 和 426%。未来的研究需要确认再利用椰糠中的去果核绵菌对草莓植株健康和果实生产力的作用,然后确定减缓产量下降的管理策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Amplicon-based metagenomics to study the effect of coir age and wood biochar on microbiome in relation to strawberry yield
In the UK, strawberry is mostly grown in coconut coir substrate under protection. Coir substrate is usually used only for one or two cropping seasons because the continuous reuse of coir without any treatment leads to yield decline. In this study, we investigated the changes in bacterial and fungal communities in strawberry roots and bulk coir in relation to (i) the coir substrate age (cropping seasons) and (ii) oak or beech biochar amendment at planting. Coir age did not affect fungal/bacterial alpha (within-sample) diversity but affected beta (between-sample) diversity. Amendment with either oak or beech biochar did not lead to significant changes in either alpha or beta diversity for both fungi and bacteria, but it did alter the relative abundance of 13 fungal ASVs. This study identified six bacterial and 20 fungal ASVs with a significant positive linear relationship with coir age and also eight bacterial and 22 fungal ASVs with a significant negative linear relationship with coir age. Notably, the observed strawberry yield decline in reused coir substrate could be associated with a generalist root pathogen, Ilyonectria destructans (ex. Cylindrocarpon destructans), of which the abundance increased annually by 225% and 426% in strawberry root and bulk coir, respectively. Future research is needed to confirm the role of I. destructans in reused coir on strawberry plant health and fruit productivity and then to identify management strategies for yield decline mitigation.
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来源期刊
Frontiers in Agronomy
Frontiers in Agronomy Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
4.80
自引率
0.00%
发文量
123
审稿时长
13 weeks
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