根据碘积累水平研究淡水藻小叶藻的吸收特性

R. Ryvak, T. Synyshyna, H. Harvas
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摘要

文章介绍了藻类从水环境中集中积累生物量并积聚其中所含矿物质元素的独特特性,阐述了淡水和海洋藻类吸附能力研究方向的依据、实验方案、所用材料和方法。淡水藻类 Lemna minor 的吸附能力研究是在使用荧光灯 24 小时照明的活体饲养室中进行的,环境温度为 28-30 °C,空气湿度为 75-80%,玻璃容器中的湖水温度在 22-25 °С 之间变化。将等量的淡水藻类 Lemna minor 放入配制好的水培养基中,培养基中加入不同浓度的碘化钾形式的碘。分别在实验开始、15 天后和实验结束时测定干湿藻类生物量中的碘含量。为了获得干生物量,将含有不同浓度碘的测试样本在 85 ºС 温度下烘干。为了测定水芹生物质中的碘含量,使用了带有负极性高压源的 Kapel-105M 毛细管电泳系统。在实验过程中,无论是在栽培 15 天后还是在实验结束时,都发现小水芹干生物量中的碘含量显著增加。根据对淡水藻类莱姆纳(Lemna minor)生物量的吸附能力与碘积累水平的关系的研究结果,确定了这种藻类在其生物量中积累碘含量的能力取决于培养基中的碘浓度。当浮萍在碘浓度为 40-2000 毫克/立方米的培养基上生长时,其生物量中的碘积累强度最高。培养基中高浓度的碘对浮萍的吸附特性有负面影响,浮萍中碘的积累、生长延迟以及在实验结束时导致浮萍死亡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
STUDY OF ABSORBENT PROPERTIES OF FRESHWATER ALGAE LEMNA MINOR ACCORDING TO THE LEVEL OF IODINE ACCUMULATION
The article describes the unique properties of algae to intensively build up biomass and accumulate the mineral elements present in it from the water environment, the justification of the direction of research on the sorption capacity of freshwater and marine algae, the scheme of the experiment, used materials and methods are described. The study of the sorption capacity of the freshwater algae Lemna minor was carried out in a vivarium under 24-hour lighting using fluorescent lamps, the ambient temperature was 28-30 °C, the air humidity was 75-80%, the temperature of the lake water in glass containers varied between 22-25 ºС. An equal mass of freshwater algae Lemna minor was placed in the prepared water medium with different concentrations of iodine in the form of potassium iodide. The content of iodine in wet and dry algae biomass was determined at the beginning of the experiment, after 15 days and at the end of the experiment. To obtain dry biomass, test samples with different concentrations of iodine were dried at a temperature of 85 ºС. To determine the content of iodine in watercress biomass, the capillary electrophoresis method was used using the Kapel-105M capillary electrophoresis system with a negative polarity high voltage source. In the course of the experiment, a significant increase in the iodine content in the dry biomass of Lemna minor was noted both after 15 days of cultivation and at the end of the experiment. Based on the results of studies of the sorption capacity of the biomass of the freshwater algae Lemna minor according to the level of iodine accumulation, the ability of this algae to accumulate iodine content in its biomass, depending on the concentration of iodine in the cultivation medium, was established. The highest intensity of iodine accumulation in duckweed biomass was noted when it was grown on cultivation media with an iodine concentration of 40-2000 mg/dm3. High concentrations of Iodine in the culture medium had a negative effect on the sorption properties of Lemna minor, accumulation of Iodine in it, delayed growth, and by the end of the experiment caused its death.
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