用于定量酶联免疫吸附试验的微流体纸基侧流装置

Micro Pub Date : 2024-05-16 DOI:10.3390/micro4020022
Ashutosh Kumar, Cameron Hahn, Stephen Herchen, Alex Soucy, Ethan Carpio, Sophia Harper, Nassim Rahmani, Constantine Anagnostopoulos, Mohammad Faghri
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引用次数: 0

摘要

本研究提出了一种创新的横向流微流体纸基分析装置(μPAD),设计用于进行定量纸基酶联免疫吸附测定(p-ELISA),以纸基形式无缝执行传统的酶联免疫吸附测定步骤。p-ELISA 装置采用了无源流体电路,其功能元件包括多生物材料悬臂 (B-MaC) 组件、延迟通道和缓冲区,所有这些元件都封闭在外壳内,用于将关键试剂按顺序自主装载到检测区。这种新方法不仅能在 30 分钟内快速完成检测,还能降低试剂需求、减少设备需求,并广泛适用于临床诊断和环境监测。本手稿详细描述了多向流动分析仪(MDFA)的设计、材料和制造方法,重点介绍了该装置以用户友好的多功能形式进行复杂生化分析的潜力。分析性能评估(包括兔 IgG 8.4 pM 的检测限 (LOD))确定了该装置与现有 p-ELISA 方法相比的功效。这项开创性工作为未来自主诊断技术的发展奠定了基础,旨在通过便捷可靠的检测解决方案提高全球健康水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Microfluidic Paper-Based Lateral Flow Device for Quantitative ELISA
This study presents an innovative lateral flow microfluidic paper-based analytical device (μPAD) designed for conducting quantitative paper-based enzyme-linked immunosorbent assays (p-ELISA), seamlessly executing conventional ELISA steps in a paper-based format. The p-ELISA device utilizes a passive fluidic circuit with functional elements such as a multi-bi-material cantilever (B-MaC) assembly, delay channels, and a buffer zone, all enclosed within housing for autonomous, sequential loading of critical reagents onto the detection zone. This novel approach not only demonstrates a rapid assay completion time of under 30 min, but also boasts reduced reagent requirements, minimal equipment needs, and broad applicability across clinical diagnostics and environmental surveillance. Through detailed descriptions of the design, materials, and fabrication methods for the multi-directional flow assay (MDFA), this manuscript highlights the device’s potential for complex biochemical analyses in a user-friendly and versatile format. Analytical performance evaluation, including a limit of detection (LOD) of 8.4 pM for Rabbit IgG, benchmarks the device’s efficacy compared to existing p-ELISA methodologies. This pioneering work lays the groundwork for future advancements in autonomous diagnostics, aiming to enhance global health outcomes through accessible and reliable testing solutions.
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