Marios C. Christodoulou, Diego J. Gonzalez‐Serrano, A. Christou, Ioannis J. Stavrou, Milad Hadidi, Andres Moreno, Constantina P. Kapnissi‐Christodoulou
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Optimization of microwave‐assisted extraction for quantification of cannabinoids in hemp tea by liquid chromatography‐mass spectrometry
Microwave‐assisted extraction (MAE) of cannabinoids from hemp tea was optimized, for the first, using response surface methodology. The effect of temperature (50, 65, and 80°C), irradiation time (4, 7, and 10 min), and solvent‐to‐solid ratio (20, 30, and 40 mL of methanol/g of hemp tea) on cannabinoid extractability were investigated. The concentrations of five cannabinoids, namely Δ9‐tetrahydrocannabinol (Δ9‐THC), cannabidiol (CBD), cannabigerol (CBG), cannabichromene (CBC), and cannabinol (CBN), were selected as response variables. For the quantitative analysis, a liquid chromatography‐mass spectrometry method was developed and validated. The proposed analytical approach demonstrated satisfactory performance characteristics in terms of linearity (R2 ≥ 0.9998), precision (intra‐day: 1.99%–5.97% relative standard deviation [%RSD], inter‐day: 1.95%–6.08%RSD), sensitivity (limit of detection: 1.35–2.36 ng/g, limit of quantification: 4.05–7.08 ng/g) and carry‐over effect (signals ≤ 5.03%), with all cannabinoids eluting within 6 min. For comparison purposes, soxhlet extraction, ultrasound‐assisted extraction (UAE), and conventional‐stirring extraction were additionally performed. MAE proved to be a more effective technique for the extraction of CBD and CBN, while UAE managed to extract Δ9‐THC, CBG, and CBC at higher concentration levels.
期刊介绍:
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.