{"title":"暴露于金属基纳米材料的 THP-1 细胞的基因表达谱和蛋白质相互作用网络。","authors":"Šíma Michal, Líbalová Helena, Závodná Táňa, Vrbová Kristýna, Kléma Jiří, Rössner Pavel","doi":"10.1016/j.etap.2024.104469","DOIUrl":null,"url":null,"abstract":"<p><p>We analyzed gene expression in THP-1 cells exposed to metal-based nanomaterials (NMs) [TiO<sub>2</sub> (NM-100), ZnO (NM-110), SiO<sub>2</sub> (NM-200), Ag (NM-300 K)]. A functional enrichment analysis of the significant differentially expressed genes (DEGs) identified the key modulated biological processes and pathways. DEGs were used to construct protein-protein interaction networks. NM-110 and NM-300 K induced changes in the expression of genes involved in oxidative and genotoxic stress, immune response, alterations of cell cycle, detoxification of metal ions and regulation of redox-sensitive pathways. Both NMs shared a number of highly connected protein nodes (hubs) including CXCL8, ATF3, HMOX1, and IL1B. NM-200 induced limited transcriptional changes, mostly related to the immune response; however, several hubs (CXCL8, ATF3) were identical with NM-110 and NM-300 K. No effects of NM-100 were observed. Overall, soluble nanomaterials NM-110 and NM-300 K exerted a wide variety of toxic effects, while insoluble NM-200 induced immunotoxicity; NM-100 caused no detectable changes on the gene expression level.</p>","PeriodicalId":93992,"journal":{"name":"Environmental toxicology and pharmacology","volume":" ","pages":"104469"},"PeriodicalIF":0.0000,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Gene expression profiles and protein-protein interaction networks in THP-1 cells exposed to metal-based nanomaterials.\",\"authors\":\"Šíma Michal, Líbalová Helena, Závodná Táňa, Vrbová Kristýna, Kléma Jiří, Rössner Pavel\",\"doi\":\"10.1016/j.etap.2024.104469\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We analyzed gene expression in THP-1 cells exposed to metal-based nanomaterials (NMs) [TiO<sub>2</sub> (NM-100), ZnO (NM-110), SiO<sub>2</sub> (NM-200), Ag (NM-300 K)]. A functional enrichment analysis of the significant differentially expressed genes (DEGs) identified the key modulated biological processes and pathways. DEGs were used to construct protein-protein interaction networks. NM-110 and NM-300 K induced changes in the expression of genes involved in oxidative and genotoxic stress, immune response, alterations of cell cycle, detoxification of metal ions and regulation of redox-sensitive pathways. Both NMs shared a number of highly connected protein nodes (hubs) including CXCL8, ATF3, HMOX1, and IL1B. NM-200 induced limited transcriptional changes, mostly related to the immune response; however, several hubs (CXCL8, ATF3) were identical with NM-110 and NM-300 K. No effects of NM-100 were observed. Overall, soluble nanomaterials NM-110 and NM-300 K exerted a wide variety of toxic effects, while insoluble NM-200 induced immunotoxicity; NM-100 caused no detectable changes on the gene expression level.</p>\",\"PeriodicalId\":93992,\"journal\":{\"name\":\"Environmental toxicology and pharmacology\",\"volume\":\" \",\"pages\":\"104469\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-05-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental toxicology and pharmacology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.etap.2024.104469\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental toxicology and pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.etap.2024.104469","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
我们分析了暴露于金属基纳米材料(NMs)[TiO2(NM-100)、ZnO(NM-110)、SiO2(NM-200)、Ag(NM-300 K)]的 THP-1 细胞的基因表达。对显著差异表达基因(DEGs)的功能富集分析确定了关键的调节生物过程和途径。DEGs 被用于构建蛋白质-蛋白质相互作用网络。NM-110 和 NM-300 K 诱导了涉及氧化和基因毒性应激、免疫反应、细胞周期改变、金属离子解毒和氧化还原敏感通路调控的基因表达变化。两种 NMs 都共享一些高度连接的蛋白质节点(枢纽),包括 CXCL8、ATF3、HMOX1 和 IL1B。NM-200 诱导了有限的转录变化,主要与免疫反应有关;然而,几个枢纽(CXCL8、ATF3)与 NM-110 和 NM-300 K 相同。总之,可溶性纳米材料 NM-110 和 NM-300 K 产生了多种毒性效应,而不溶性纳米材料 NM-200 引发了免疫毒性;NM-100 在基因表达水平上没有引起可检测到的变化。
Gene expression profiles and protein-protein interaction networks in THP-1 cells exposed to metal-based nanomaterials.
We analyzed gene expression in THP-1 cells exposed to metal-based nanomaterials (NMs) [TiO2 (NM-100), ZnO (NM-110), SiO2 (NM-200), Ag (NM-300 K)]. A functional enrichment analysis of the significant differentially expressed genes (DEGs) identified the key modulated biological processes and pathways. DEGs were used to construct protein-protein interaction networks. NM-110 and NM-300 K induced changes in the expression of genes involved in oxidative and genotoxic stress, immune response, alterations of cell cycle, detoxification of metal ions and regulation of redox-sensitive pathways. Both NMs shared a number of highly connected protein nodes (hubs) including CXCL8, ATF3, HMOX1, and IL1B. NM-200 induced limited transcriptional changes, mostly related to the immune response; however, several hubs (CXCL8, ATF3) were identical with NM-110 and NM-300 K. No effects of NM-100 were observed. Overall, soluble nanomaterials NM-110 and NM-300 K exerted a wide variety of toxic effects, while insoluble NM-200 induced immunotoxicity; NM-100 caused no detectable changes on the gene expression level.