{"title":"用于检测卫生水样中军团菌属的培养基比较：适应 ISO 11731:2017。","authors":"","doi":"10.1016/j.eimce.2024.05.007","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p>Water sample culturing is the reference method to detect <span><span>Legionella</span></span><span> spp. in sanitary facilities. Until 2017, UNE-EN ISO 11731 only included the GVPC<span> medium, which inhibits interfering microbiota but hinders the growth of </span></span><em>Legionella</em> spp. To improve its recovery, the new standard incorporates the BCYE medium into the working protocol.</p></div><div><h3>Methods</h3><p><span>We inoculated 1306 sanitary water samples onto BCYE and GVPC according to an accredited internal procedure. We compared the number of cfu/L of </span><em>Legionella</em> spp. detected in both media.</p></div><div><h3>Results</h3><p><span>The median in BCYE was 2000 cfu/L higher than in GVPC (</span><em>P</em><span> = .000). In the presence of high amounts of interfering microbiota, both media were similar; in the absence<span> or low interfering microbiota BCYE was four times more sensitive than GVPC (</span></span><em>P</em> = .000).</p></div><div><h3>Conclusion</h3><p>Including BCYE in the analysis of sanitary water significantly improves the recovery of <em>Legionella</em> spp. in low contaminated samples.</p></div>","PeriodicalId":72916,"journal":{"name":"Enfermedades infecciosas y microbiologia clinica (English ed.)","volume":"42 7","pages":"Pages 373-376"},"PeriodicalIF":0.0000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of culture media for the detection of Legionella spp. in sanitary water samples: Adaptation to ISO 11731:2017\",\"authors\":\"\",\"doi\":\"10.1016/j.eimce.2024.05.007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><p>Water sample culturing is the reference method to detect <span><span>Legionella</span></span><span> spp. in sanitary facilities. Until 2017, UNE-EN ISO 11731 only included the GVPC<span> medium, which inhibits interfering microbiota but hinders the growth of </span></span><em>Legionella</em> spp. To improve its recovery, the new standard incorporates the BCYE medium into the working protocol.</p></div><div><h3>Methods</h3><p><span>We inoculated 1306 sanitary water samples onto BCYE and GVPC according to an accredited internal procedure. We compared the number of cfu/L of </span><em>Legionella</em> spp. detected in both media.</p></div><div><h3>Results</h3><p><span>The median in BCYE was 2000 cfu/L higher than in GVPC (</span><em>P</em><span> = .000). In the presence of high amounts of interfering microbiota, both media were similar; in the absence<span> or low interfering microbiota BCYE was four times more sensitive than GVPC (</span></span><em>P</em> = .000).</p></div><div><h3>Conclusion</h3><p>Including BCYE in the analysis of sanitary water significantly improves the recovery of <em>Legionella</em> spp. in low contaminated samples.</p></div>\",\"PeriodicalId\":72916,\"journal\":{\"name\":\"Enfermedades infecciosas y microbiologia clinica (English ed.)\",\"volume\":\"42 7\",\"pages\":\"Pages 373-376\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Enfermedades infecciosas y microbiologia clinica (English ed.)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2529993X24001266\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Enfermedades infecciosas y microbiologia clinica (English ed.)","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2529993X24001266","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Comparison of culture media for the detection of Legionella spp. in sanitary water samples: Adaptation to ISO 11731:2017
Introduction
Water sample culturing is the reference method to detect Legionella spp. in sanitary facilities. Until 2017, UNE-EN ISO 11731 only included the GVPC medium, which inhibits interfering microbiota but hinders the growth of Legionella spp. To improve its recovery, the new standard incorporates the BCYE medium into the working protocol.
Methods
We inoculated 1306 sanitary water samples onto BCYE and GVPC according to an accredited internal procedure. We compared the number of cfu/L of Legionella spp. detected in both media.
Results
The median in BCYE was 2000 cfu/L higher than in GVPC (P = .000). In the presence of high amounts of interfering microbiota, both media were similar; in the absence or low interfering microbiota BCYE was four times more sensitive than GVPC (P = .000).
Conclusion
Including BCYE in the analysis of sanitary water significantly improves the recovery of Legionella spp. in low contaminated samples.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
