Cheng-Jie Xie, Ling Yao, Rong Tang, Shuang Han, Shang Yang, Hend Alwathnani, Christopher Rensing, Guo-Hong Liu, Shun-Gui Zhou
{"title":"从稻田土壤中分离出的新型固氮菌--Azotosporobacter soli gen.","authors":"Cheng-Jie Xie, Ling Yao, Rong Tang, Shuang Han, Shang Yang, Hend Alwathnani, Christopher Rensing, Guo-Hong Liu, Shun-Gui Zhou","doi":"10.1007/s10482-024-01978-6","DOIUrl":null,"url":null,"abstract":"<div><p>A nitrogen-fixing strain designated SG130<sup>T</sup> was isolated from paddy soil in Fujian Province, China. Strain SG130<sup>T</sup> was Gram-staining-negative, rod-shaped, and strictly anaerobic. Strain SG130<sup>T</sup> showed the highest 16S rRNA gene sequence similarities with the type strains <i>Dendrosporobacter quercicolus</i> DSM 1736<sup>T</sup> (91.7%), <i>Anaeroarcus burkinensis</i> DSM 6283<sup>T</sup> (91.0%) and <i>Anaerospora hongkongensis</i> HKU 15<sup>T</sup> (90.9%). Furthermore, the phylogenetic and phylogenomic analysis also suggested strain SG130<sup>T</sup> clustered with members of the family <i>Sporomusaceae</i> and was distinguished from other genera within this family. Growth of strain SG130<sup>T</sup> was observed at 25–45 °C (optimum 30 °C), pH 6.0–9.5 (optimum 7.0) and 0–1% (w/v) NaCl (optimum 0.1%). The quinones were Q-8 and Q-9. The polar lipids were phosphatidylserine (PS), phosphatidylethanolamine (PE), glycolipid (GL), phospholipid (PL) and an unidentified lipid (UL). The major fatty acids (> 10%) were iso-C<sub>13:0</sub> 3OH (26.6%), iso-C<sub>17:1</sub> (15.6%) and iso-C<sub>15:1</sub> F (11.4%). The genomic DNA G + C content was 50.7%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SG130<sup>T</sup> and the most closely related type strain <i>D</i>. <i>quercicolus</i> DSM 1736<sup>T</sup> (ANI 68.0% and dDDH 20.3%) were both below the cut-off level for species delineation. The average amino acid identity (AAI) between strain SG130<sup>T</sup> and the most closely related type strain <i>D</i>. <i>quercicolus</i> DSM 1736<sup>T</sup> was 63.2%, which was below the cut-off value for bacterial genus delineation (65%). Strain SG130<sup>T</sup> possessed core genes (<i>nifHDK</i>) involved in nitrogen fixation, and nitrogenase activity (106.38 μmol C<sub>2</sub>H<sub>4</sub> g<sup>−1</sup> protein h<sup>−1</sup>) was examined using the acetylene reduction assay. Based on the above results, strain SG130<sup>T</sup> is confirmed to represent a novel genus of the family <i>Sporomusaceae</i>, for which the name <i>Azotosporobacter soli</i> gen. nov., sp. nov. is proposed. The type strain is SG130<sup>T</sup> (= GDMCC 1.3312<sup>T</sup> = JCM 35641<sup>T</sup>).</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Azotosporobacter soli gen. nov., sp. nov., a novel nitrogen-fixing bacterium isolated from paddy soil\",\"authors\":\"Cheng-Jie Xie, Ling Yao, Rong Tang, Shuang Han, Shang Yang, Hend Alwathnani, Christopher Rensing, Guo-Hong Liu, Shun-Gui Zhou\",\"doi\":\"10.1007/s10482-024-01978-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A nitrogen-fixing strain designated SG130<sup>T</sup> was isolated from paddy soil in Fujian Province, China. Strain SG130<sup>T</sup> was Gram-staining-negative, rod-shaped, and strictly anaerobic. Strain SG130<sup>T</sup> showed the highest 16S rRNA gene sequence similarities with the type strains <i>Dendrosporobacter quercicolus</i> DSM 1736<sup>T</sup> (91.7%), <i>Anaeroarcus burkinensis</i> DSM 6283<sup>T</sup> (91.0%) and <i>Anaerospora hongkongensis</i> HKU 15<sup>T</sup> (90.9%). Furthermore, the phylogenetic and phylogenomic analysis also suggested strain SG130<sup>T</sup> clustered with members of the family <i>Sporomusaceae</i> and was distinguished from other genera within this family. Growth of strain SG130<sup>T</sup> was observed at 25–45 °C (optimum 30 °C), pH 6.0–9.5 (optimum 7.0) and 0–1% (w/v) NaCl (optimum 0.1%). The quinones were Q-8 and Q-9. The polar lipids were phosphatidylserine (PS), phosphatidylethanolamine (PE), glycolipid (GL), phospholipid (PL) and an unidentified lipid (UL). The major fatty acids (> 10%) were iso-C<sub>13:0</sub> 3OH (26.6%), iso-C<sub>17:1</sub> (15.6%) and iso-C<sub>15:1</sub> F (11.4%). The genomic DNA G + C content was 50.7%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SG130<sup>T</sup> and the most closely related type strain <i>D</i>. <i>quercicolus</i> DSM 1736<sup>T</sup> (ANI 68.0% and dDDH 20.3%) were both below the cut-off level for species delineation. The average amino acid identity (AAI) between strain SG130<sup>T</sup> and the most closely related type strain <i>D</i>. <i>quercicolus</i> DSM 1736<sup>T</sup> was 63.2%, which was below the cut-off value for bacterial genus delineation (65%). Strain SG130<sup>T</sup> possessed core genes (<i>nifHDK</i>) involved in nitrogen fixation, and nitrogenase activity (106.38 μmol C<sub>2</sub>H<sub>4</sub> g<sup>−1</sup> protein h<sup>−1</sup>) was examined using the acetylene reduction assay. Based on the above results, strain SG130<sup>T</sup> is confirmed to represent a novel genus of the family <i>Sporomusaceae</i>, for which the name <i>Azotosporobacter soli</i> gen. nov., sp. nov. is proposed. The type strain is SG130<sup>T</sup> (= GDMCC 1.3312<sup>T</sup> = JCM 35641<sup>T</sup>).</p></div>\",\"PeriodicalId\":50746,\"journal\":{\"name\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"volume\":\"117 1\",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-05-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10482-024-01978-6\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10482-024-01978-6","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Azotosporobacter soli gen. nov., sp. nov., a novel nitrogen-fixing bacterium isolated from paddy soil
A nitrogen-fixing strain designated SG130T was isolated from paddy soil in Fujian Province, China. Strain SG130T was Gram-staining-negative, rod-shaped, and strictly anaerobic. Strain SG130T showed the highest 16S rRNA gene sequence similarities with the type strains Dendrosporobacter quercicolus DSM 1736T (91.7%), Anaeroarcus burkinensis DSM 6283T (91.0%) and Anaerospora hongkongensis HKU 15T (90.9%). Furthermore, the phylogenetic and phylogenomic analysis also suggested strain SG130T clustered with members of the family Sporomusaceae and was distinguished from other genera within this family. Growth of strain SG130T was observed at 25–45 °C (optimum 30 °C), pH 6.0–9.5 (optimum 7.0) and 0–1% (w/v) NaCl (optimum 0.1%). The quinones were Q-8 and Q-9. The polar lipids were phosphatidylserine (PS), phosphatidylethanolamine (PE), glycolipid (GL), phospholipid (PL) and an unidentified lipid (UL). The major fatty acids (> 10%) were iso-C13:0 3OH (26.6%), iso-C17:1 (15.6%) and iso-C15:1 F (11.4%). The genomic DNA G + C content was 50.7%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain SG130T and the most closely related type strain D. quercicolus DSM 1736T (ANI 68.0% and dDDH 20.3%) were both below the cut-off level for species delineation. The average amino acid identity (AAI) between strain SG130T and the most closely related type strain D. quercicolus DSM 1736T was 63.2%, which was below the cut-off value for bacterial genus delineation (65%). Strain SG130T possessed core genes (nifHDK) involved in nitrogen fixation, and nitrogenase activity (106.38 μmol C2H4 g−1 protein h−1) was examined using the acetylene reduction assay. Based on the above results, strain SG130T is confirmed to represent a novel genus of the family Sporomusaceae, for which the name Azotosporobacter soli gen. nov., sp. nov. is proposed. The type strain is SG130T (= GDMCC 1.3312T = JCM 35641T).
期刊介绍：
Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
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