鼻窦呼吸道上皮腺瘤样火腿肠瘤与鼻息肉的区别:免疫病理学的启示。

IF 4.8 2区 医学 Q1 OTORHINOLARYNGOLOGY
Rhinology Pub Date : 2024-08-01 DOI:10.4193/Rhin23.405
X Du, M Zhang, S Zhang, X Yan, L Wang, Z Zhang, N Li, L Yu, Y Jiang
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引用次数: 0

摘要

背景:呼吸道上皮腺瘤样火腿肠瘤(REAH)是一种常见于鼻腔和鼻窦的良性病变。它通常伴有鼻息肉(NP)。虽然对这两种疾病的组织学特征已有研究,但对它们在潜在免疫病理学方面的差异了解有限:方法:从 8 名同时患有 REAH 和 NP 的患者和 10 名对照组患者身上采集鼻腔组织标本。分析了组织中炎性细胞因子、紧密连接(TJ)和上皮-间质转化(EMT)相关因子的表达水平。采用 qRT-PCR 检测上述因子的 mRNA 表达,并通过 Western 印迹和免疫组化分析 TJ 和 EMT 相关蛋白的表达:结果:与对照组相比,REAH和NP组织中炎性细胞因子(IFN-γ、IL-5、IL-17A、IL-31、IL-33和TNF-α)和EMT相关因子(α-SMA、COL1A1、MMP9、TGF-β1和Vimentin)的水平均显著升高。相反,E-Cadherin 和 TJ 相关因子(Claudin-4 和 Occludin)则明显减少。比较 REAH 和 NP,发现 REAH 中 IL-4、IL-5 和 IL-33 的表达较低,而 TNF-α 的表达较高。在TJ相关因子方面,REAH中Occludin的表达较低。此外,在 EMT 相关因子方面,除 E-Cadherin 外,α-SMA、COL1A1、CTGF、MMP9、TGF-β1 和 Vimentin 的表达在 REAH 中较高:结论:REAH和NP表现出不同的免疫病理机制。结论:REAH 和 NP 表现出不同的免疫病理机制,NP 表现出更严重的炎症反应,而 REAH 的特点是 TJ 和 EMT 破坏比 NP 更明显。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The differences between sinonasal respiratory epithelial adenomatoid hamartoma and nasal polyps: insights into immunopathology.

Background: Respiratory epithelial adenomatoid hamartoma (REAH) is a benign lesion commonly occurring in the nasal cavity and sinuses. It is often accompanied by nasal polyps (NP). While the histological features of these two conditions have been studied, there is limited knowledge about their differences in the underlying immunopathology.

Methods: Nasal tissue specimens were collected from 8 patients with concurrent REAH and NP and 10 controls. The expression levels of inflammatory cytokines, tight junctions (TJ), and epithelial-mesenchymal transition (EMT)-related factors in the tissues were analyzed. The mRNA expression of the aforementioned factors was measured using qRT-PCR, while the expression of TJ and EMT-related proteins was analyzed through Western blotting and immunohistochemistry.

Results: Compared to the control group, levels of inflammatory cytokines (IFN-α, IL-5, IL-17A, IL-31, IL-33, and TNF-α) and EMT-related factors (α-SMA, COL1A1, MMP9, TGF-β1, and Vimentin) were significantly increased in both REAH and NP tissues. Conversely, E-Cadherin and TJ-related factors (Claudin-4 and Occludin) significantly decreased. When comparing REAH with NP, it was observed that the expression of IL-4, IL-5, and IL-33 was lower in REAH, while TNF-ɑ; was higher. Regarding TJ-related factors, the expression of Occludin was lower in REAH. Furthermore, in terms of EMT-related factors, except for E-Cadherin, the expressions of ɑ-SMA, COL1A1, CTGF, MMP9, TGF-β11, and Vimentin were higher in REAH.

Conclusion: REAH and NP exhibit different immunopathological mechanisms. NP demonstrates a more severe inflammatory response, whereas REAH is characterized by a more pronounced TJ and EMT breakdown than NP.

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来源期刊
Rhinology
Rhinology 医学-耳鼻喉科学
CiteScore
15.80
自引率
9.70%
发文量
135
审稿时长
6-12 weeks
期刊介绍: Rhinology serves as the official Journal of the International Rhinologic Society and is recognized as one of the journals of the European Rhinologic Society. It offers a prominent platform for disseminating rhinologic research, reviews, position papers, task force reports, and guidelines to an international scientific audience. The journal also boasts the prestigious European Position Paper in Rhinosinusitis (EPOS), a highly influential publication first released in 2005 and subsequently updated in 2007, 2012, and most recently in 2020. Employing a double-blind peer review system, Rhinology welcomes original articles, review articles, and letters to the editor.
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