生姜根生物活性化合物能有效抑制培养的结肠癌细胞生长

IF 2.3 Q3 NUTRITION & DIETETICS
Nutrition and Metabolic Insights Pub Date : 2024-05-15 eCollection Date: 2024-01-01 DOI:10.1177/11786388241231163
Shelley Lin, Peiran Lu
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引用次数: 0

摘要

目的:结肠癌在很多人中都很常见,癌症给很多人的生活带来了极大的影响。生姜是一种常见的食物,尤其是在亚洲菜肴中。然而,生姜作为一种完整的食物及其生物活性化合物 6-姜酚对预防结肠癌的健康益处尚未得到充分研究。本实验研究了姜汁和 6-姜酚对结肠癌细胞生长和死亡的影响:方法:将新鲜生姜根茎匀浆制汁。方法:将新鲜生姜根茎匀浆制汁,用 Folin-C 法测定姜汁中的总酚含量。用姜汁和/或 6-gingerol 处理结肠癌 SW480 细胞和正常结肠上皮细胞 CCD-18Co。细胞代谢活性通过 MTT 法进行评估。用免疫印迹法检测细胞凋亡和细胞周期停滞。数据采用双因素方差分析和 Tukey 后检验,统计学显著性以 P 为标准:结果表明,生姜汁在 25 µL/mL 浓度下可选择性抑制 SW480 细胞生长 40 小时。高剂量姜汁(50 和 100 µL/mL 40 小时)抑制了两种细胞类型的生长。这与 Caspase-3 的激活无关。从 0.5 µmoL/L开始,六姜酚可特异性抑制 SW480 细胞的生长(P P > .1)。免疫印迹结果显示,用 1 和/或 2.5 µmoL/L 6-gingerol 处理 40 小时后,Myt1 水平升高,CDK1、p21 Wafl/Cip1 和 pSer642-Wee1 水平下降,仅发生在 SW480 细胞中,而不发生在 CCD-18Co 细胞中。姜汁能在 ~25 µL/mL 的窄窗口内选择性抑制结肠癌细胞的生长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ginger Root Bioactive Compounds Specifically Inhibits Growth of Colon Cancer Cells in Culture.

Objective: Colon cancer is affluent among many people, and having cancer greatly impacts the lives of many. Ginger is a common food, particularly in Asian cuisine. However, the health benefits of ginger as a whole food and 6-gingerol, its bioactive compound in prevention of colon cancer have not been fully addressed. This experiment investigated effects of ginger juice and 6-gingerol on colon cancer cell growth and death.

Methods: Fresh ginger roots were homogenized for juice preparation. Total phenolic contents of ginger juice were measured using Folin-C assay. Colon cancer SW480 cells and normal colon epithelial cells CCD-18Co were treated with ginger juice and/or 6-gingerol. Cell metabolic activity was assessed by MTT assay. Cell apoptosis and cell cycle arrest were accessed by immunoblotting. Data were analyzed by 2-way ANOVA with a Tukey post-hoc test and statistical significance was set at P < .05.

Results: The results showed that ginger juice selectively inhibited SW480 cell growth at 25 µL/mL for 40 hours. High doses of ginger juice (at 50 and 100 µL/mL for 40 hours) inhibited the growth of both cell types. This was independent of caspase-3 activation. Six-gingerol specifically inhibited SW480 cell growth starting at 0.5 µmoL/L (P < .01). More than 1 µmoL/L 6-gingerol did not give more power to inhibit SW480 cell growth. The results also showed that CCD-18Co cell growth rates were not changed after 6-gingerol treatments (up to 10 µmoL/L, P > .1). Immunoblotting results revealed that the elevation of Myt1 levels and decreases in CDK1, p21 Wafl/Cip1 and pSer642-Wee1 only occurred in SW480 but not CCD-18Co cells when treated with 1 and/or 2.5 µmoL/L 6-gingerol for 40 hours.

Conclusion: 6-gingerol can specifically inhibit SW480 cancer cells without killing normal CCd-18Co cells, through cell cycle arrest. Ginger juice can selectively inhibit colon cancer cell growth in a narrow window at ~25 µL/mL.

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来源期刊
Nutrition and Metabolic Insights
Nutrition and Metabolic Insights NUTRITION & DIETETICS-
CiteScore
3.30
自引率
0.00%
发文量
27
审稿时长
8 weeks
期刊介绍: Nutrition and Metabolic Insights is a peer-reviewed, open-access online journal focusing on all aspects of nutrition and metabolism. This encompasses nutrition, including the biochemistry of metabolism, exercise and associated physical processes and also includes clinical articles that relate to metabolism, such as obesity, lipidemias and diabetes. It includes research at the molecular, cellular and organismal levels. This journal welcomes new manuscripts for peer review on the following topics: Nutrition, including the biochemistry of metabolism, Exercise and associated physical processes, Clinical articles that relate to metabolism, such as obesity, lipidemias and diabetes, Research at the molecular, cellular and organismal levels, Other areas of interest include gene-nutrient interactions, the effects of hormones, models of metabolic function, macronutrient interactions, outcomes of changes in diet, and pathophysiology.
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