大豆[Glycine max (L.) Merr.]中大豆花叶病毒株 SC7 抗性基因座和候选基因的鉴定。

IF 2.3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yixiang Pu, Rujuan Yan, Dongbing Jia, Zhijun Che, Rufei Yang, Changyun Yang, Hui Wang, Hao Cheng, Deyue Yu
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引用次数: 0

摘要

大豆(Glycine max (L.) Merr.)是世界上重要的豆科作物,为人类提供了丰富的植物蛋白和油脂。大豆花叶病毒(SMV)会对大豆的产量和品质造成严重危害,但很难用化学药剂控制 SMV,培育抗 SMV 的品种已成为控制该病害的最有效途径。因此,从大豆资源中鉴定 SMV 抗性基因并将其应用于大豆育种具有重要意义。本研究调查了 219 个大豆品种在两种环境中对 SMV 株系 SC7 的发病率(DRs)。利用高密度 NJAU 355 K SoySNP 阵列对 DR 进行了全基因组关联研究(GWAS)。在两个环境中重复检测了 15 号染色体上的 274 kb 区域(1,110,567 bp 至 1,384,173 bp)。在 15 号染色体上发现了六个新的重要单核苷酸多态性(SNPs)。其中四个 SNP 位于两个候选基因 Glyma.15G015700 和 Glyma.15G015800。具有低 DR 的精英单倍型 Glyma.15G015700Hap I 对 SC7 表现出很强的抗性。在接种 SC7 后,抗 SMV 群体中 Glyma.15G015700 的表达量显著增加。此外,预测与 Glyma.15G015700 相互作用的蛋白大多是热休克蛋白,而热休克蛋白已被证明与抗病性有关。总之,研究发现了新的 SMV 抗性基因座和新的候选基因 Glyma.15G015700,可用于进一步的大豆抗病育种。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Identification of soybean mosaic virus strain SC7 resistance loci and candidate genes in soybean [Glycine max (L.) Merr.].

Identification of soybean mosaic virus strain SC7 resistance loci and candidate genes in soybean [Glycine max (L.) Merr.].

Soybean [Glycine max (L.) Merr.] is an important legume crop worldwide, which provides abundant plant protein and oil for human beings. Soybean mosaic virus (SMV) can cause serious damage to the yield and quality of soybean, but it is difficult to control SMV with chemicals, breeding SMV-resistant varieties has become the most effective way to control the disease. Therefore, it is important to identify SMV resistance genes from soybean resources and apply them to soybean breeding. In this study, the disease rates (DRs) of 219 soybean accessions to SMV strain SC7 in two environments were investigated. A high-density NJAU 355 K SoySNP array was used for genome-wide association study (GWAS) of DR. A 274 kb region on chromosome 15 (1,110,567 bp to 1,384,173 bp) was repeatedly detected in two environments. Six new significant single nucleotide polymorphisms (SNPs) on chromosome 15 were identified. Four of these six SNPs were located within two candidate genes, Glyma.15G015700 and Glyma.15G015800. The elite haplotype Glyma.15G015700Hap I with low DR exhibited strong resistance to SC7. The expression of Glyma.15G015700 in the SMV-resistant accession increased significantly after inoculation with SC7. Furthermore, most of the proteins predicted to interact with Glyma.15G015700 are heat shock proteins, which have been shown to be related to disease resistance. In summary, new SMV resistance loci and a new candidate gene, Glyma.15G015700, were identified and might be utilized in further soybean disease resistance breeding.

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来源期刊
Molecular Genetics and Genomics
Molecular Genetics and Genomics 生物-生化与分子生物学
CiteScore
5.10
自引率
3.20%
发文量
134
审稿时长
1 months
期刊介绍: Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic. MGG publishes research on all organisms that is of broad interest to those working in the fields of genetics, genomics, biology, medicine and biotechnology. The journal investigates a broad range of topics, including these from recent issues: mechanisms for extending longevity in a variety of organisms; screening of yeast metal homeostasis genes involved in mitochondrial functions; molecular mapping of cultivar-specific avirulence genes in the rice blast fungus and more.
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