Satomi Takei , Yoko Tabe , Takashi Miida , Tomomi Hishinuma , Abdullah Khasawneh , Teruo Kirikae , Jeevan B. Sherchand , Tatsuya Tada
{"title":"尼泊尔产生 NDM 型和 OXA 型碳青霉烯酶的耐多药肺炎克雷伯菌临床分离株。","authors":"Satomi Takei , Yoko Tabe , Takashi Miida , Tomomi Hishinuma , Abdullah Khasawneh , Teruo Kirikae , Jeevan B. Sherchand , Tatsuya Tada","doi":"10.1016/j.jgar.2024.04.008","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>The emergence of multidrug-resistant <em>Klebsiella pneumoniae</em> has become a serious problem in medical settings worldwide.</p></div><div><h3>Methods</h3><p>A total of 46 isolates of multidrug-resistant <em>K. pneumoniae</em> were obtained from 2 hospitals in Nepal from October 2018 to April 2019.</p></div><div><h3>Results</h3><p>Most of these isolates were highly resistant to carbapenems, aminoglycosides, and fluoroquinolones with the minimum inhibitory concentrations (MICs) of more than 64 µg/mL. These isolates harboured carbapenemase-encoding genes, including <em>bla</em><sub>NDM-1</sub>, <em>bla</em><sub>NDM-5</sub>, <em>bla</em><sub>OXA-181</sub> and <em>bla</em><sub>OXA-232</sub>, and 16S rRNA methyltransferase-encoding genes, including <em>armA, rmtB, rmtC,</em> and <em>rmtF</em>. Multilocus sequence typing revealed that 44 of 46 isolates were high-risk clones such as ST11 (2%), ST14 (4%), ST15 (11%), ST37 (2%), ST101 (2%), ST147 (28%), ST231 (13%), ST340 (4%), and ST395 (28%). In particular, ST395 isolates, which spread across medical settings in Nepal, co-harboured <em>bla</em><sub>NDM-5</sub> and <em>rmtB</em> on IncFII plasmids and co-harboured <em>bla</em><sub>OXA-181/-232</sub> and <em>rmtF</em> on ColKP3 plasmids. Several isolates harboured <em>bla</em><sub>OXA-181</sub> or <em>bla</em><sub>NDM-5</sub> on their chromosomes and multi-copies of <em>bla</em><sub>NDM-1</sub> or genes encoding 16S rRNA methyltransferases on their plasmids.</p></div><div><h3>Conclusions</h3><p>The presented study demonstrates that the high-risk clones of multidrug-resistant <em>K. pneumoniae</em> spread in a clonal manner across hospitals in Nepal.</p></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213716524000821/pdfft?md5=e22577e1ac53eeab526351b66832ce63&pid=1-s2.0-S2213716524000821-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Multidrug-resistant Klebsiella pneumoniae clinical isolates producing NDM- and OXA-type carbapenemase in Nepal\",\"authors\":\"Satomi Takei , Yoko Tabe , Takashi Miida , Tomomi Hishinuma , Abdullah Khasawneh , Teruo Kirikae , Jeevan B. Sherchand , Tatsuya Tada\",\"doi\":\"10.1016/j.jgar.2024.04.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>The emergence of multidrug-resistant <em>Klebsiella pneumoniae</em> has become a serious problem in medical settings worldwide.</p></div><div><h3>Methods</h3><p>A total of 46 isolates of multidrug-resistant <em>K. pneumoniae</em> were obtained from 2 hospitals in Nepal from October 2018 to April 2019.</p></div><div><h3>Results</h3><p>Most of these isolates were highly resistant to carbapenems, aminoglycosides, and fluoroquinolones with the minimum inhibitory concentrations (MICs) of more than 64 µg/mL. These isolates harboured carbapenemase-encoding genes, including <em>bla</em><sub>NDM-1</sub>, <em>bla</em><sub>NDM-5</sub>, <em>bla</em><sub>OXA-181</sub> and <em>bla</em><sub>OXA-232</sub>, and 16S rRNA methyltransferase-encoding genes, including <em>armA, rmtB, rmtC,</em> and <em>rmtF</em>. Multilocus sequence typing revealed that 44 of 46 isolates were high-risk clones such as ST11 (2%), ST14 (4%), ST15 (11%), ST37 (2%), ST101 (2%), ST147 (28%), ST231 (13%), ST340 (4%), and ST395 (28%). In particular, ST395 isolates, which spread across medical settings in Nepal, co-harboured <em>bla</em><sub>NDM-5</sub> and <em>rmtB</em> on IncFII plasmids and co-harboured <em>bla</em><sub>OXA-181/-232</sub> and <em>rmtF</em> on ColKP3 plasmids. Several isolates harboured <em>bla</em><sub>OXA-181</sub> or <em>bla</em><sub>NDM-5</sub> on their chromosomes and multi-copies of <em>bla</em><sub>NDM-1</sub> or genes encoding 16S rRNA methyltransferases on their plasmids.</p></div><div><h3>Conclusions</h3><p>The presented study demonstrates that the high-risk clones of multidrug-resistant <em>K. pneumoniae</em> spread in a clonal manner across hospitals in Nepal.</p></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2213716524000821/pdfft?md5=e22577e1ac53eeab526351b66832ce63&pid=1-s2.0-S2213716524000821-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524000821\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524000821","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Multidrug-resistant Klebsiella pneumoniae clinical isolates producing NDM- and OXA-type carbapenemase in Nepal
Objectives
The emergence of multidrug-resistant Klebsiella pneumoniae has become a serious problem in medical settings worldwide.
Methods
A total of 46 isolates of multidrug-resistant K. pneumoniae were obtained from 2 hospitals in Nepal from October 2018 to April 2019.
Results
Most of these isolates were highly resistant to carbapenems, aminoglycosides, and fluoroquinolones with the minimum inhibitory concentrations (MICs) of more than 64 µg/mL. These isolates harboured carbapenemase-encoding genes, including blaNDM-1, blaNDM-5, blaOXA-181 and blaOXA-232, and 16S rRNA methyltransferase-encoding genes, including armA, rmtB, rmtC, and rmtF. Multilocus sequence typing revealed that 44 of 46 isolates were high-risk clones such as ST11 (2%), ST14 (4%), ST15 (11%), ST37 (2%), ST101 (2%), ST147 (28%), ST231 (13%), ST340 (4%), and ST395 (28%). In particular, ST395 isolates, which spread across medical settings in Nepal, co-harboured blaNDM-5 and rmtB on IncFII plasmids and co-harboured blaOXA-181/-232 and rmtF on ColKP3 plasmids. Several isolates harboured blaOXA-181 or blaNDM-5 on their chromosomes and multi-copies of blaNDM-1 or genes encoding 16S rRNA methyltransferases on their plasmids.
Conclusions
The presented study demonstrates that the high-risk clones of multidrug-resistant K. pneumoniae spread in a clonal manner across hospitals in Nepal.
期刊介绍：
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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