印尼传统发酵牛肉(Cangkuk)中血管紧张素转换酶(ACE)抑制肽的纯化及抗高血压作用。

IF 2.4 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animal Bioscience Pub Date : 2024-10-01 Epub Date: 2024-05-07 DOI:10.5713/ab.23.0433
Irdha Mirdhayati, Wieda Nurwidada Haritsah Zain, Abdul Fatah, Issei Yokoyama, Keizo Arihara
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引用次数: 0

摘要

目的:传统发酵肉制品可被视为生物活性肽的来源。印尼传统发酵牛肉产品 Cangkuk 是 ACE 抑制肽的来源之一。本研究旨在鉴定 Cangkuk 中的 ACE 抑制肽,并分析其抗高血压作用:方法:使用多种浓度的乙醇溶剂和 OASIS HLB 滤芯进行固相萃取,然后使用 RP-HPLC 进行纯化,对苍术的水溶性部分进行分馏,以获得 ACE 抑制肽。使用 HPLC-MS 鉴定目标肽,然后使用自动蛋白质测序仪分析检测肽序列。对水溶性部分和合成肽的抗高血压作用进行了分析。动物模型由 14-16 周大的雄性自发性高血压大鼠(SHRs)[平均体重约为 320 克]组成,其平均收缩压(SBPs)高于 190 毫米汞柱。所有肽的口服剂量均为 1 mL。蒸馏水用作对照。通过测量口服后 0、2、4、6、8 和 24 小时的 SBP,观察样品和对照组的降压活性:结果:发现了两种序列的 ACE 抑制肽:EAPLNPKANR(IC50 值为 44.6 µmol L-1)和 IVG(IC50 值为 97.3 µmol L-1)。以 100 毫克/千克体重的剂量口服水溶性成分后,对 SHR 具有降压作用,用药 8 小时后,SBP 最大降低了 14.9 毫米汞柱。三肽 IVG 的 SBP 降低幅度最大,用药 8 小时后为 24.76±2.1 mm Hg。十肽 EAPLNPKANR 的 SBP 降低幅度最大,用药 8 小时后为 21.0±1.9 mm Hg。所有样本与对照组相比均有明显差异(p结论:苍术作为一种功能性食品配料,具有降压作用的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Purification of angiotensin converting enzyme inhibitory peptides and antihypertensive effect generated from Indonesian traditional fermented beef (Cangkuk).

Objective: Traditional fermented meat products can be considered a source of bioactive peptides. Cangkuk, a traditional Indonesian fermented beef product is one source of angiotensin converting enzyme (ACE) inhibitory peptides. This study aimed to identify ACE-inhibitory peptides from Cangkuk and analyze their antihypertensive effects.

Methods: The water-soluble fraction of Cangkuk was fractionated to obtain ACE-inhibitory peptides using an ethanol solvent at several concentrations and solid-phase extraction with an OASIS HLB cartridge followed by purification with reversed-phase high-performance liquid chromatography (RP-HPLC). HPLC-MS was used to identify target peptides, followed by automatic protein sequencer analysis to detect peptide sequences. Antihypertensive effects were analyzed on the water-soluble fraction and synthesized peptides. The animal model comprised 14-16-week-old male spontaneously hypertensive rats (SHRs) (~320 g average body weight) with mean systolic blood pressures (SBPs) higher than 190 mmHg. All oral doses of peptides were 1 mL in volume. Distilled water was used as a control. The antihypertensive activities of the sample and control were observed by measuring the SBP at 0, 2, 4, 6, 8 and 24 h after oral administration.

Results: Two sequences of ACE inhibitory peptides were found, EAPLNPKANR (IC50 value of 44.6 μmol/L) and IVG (IC50 value of 97.3 μmol/L). The water-soluble fraction demonstrated an antihypertensive effect on SHRs after oral administration at 100 mg/kg body weight, maximally lowering the SBP by 14.9 mmHg 8 h after administration. The tripeptide IVG showed the highest reduction of SBP, 24.76±2.1 mmHg 8 h after administration. The decapeptide EAPLNPKANR showed the highest reduction of SBP, 21.0±1.9 mmHg, 8 h after administration. All the samples differed significantly from the control (p<0.01).

Conclusion: Cangkuk has potential as a functional food ingredient acting as an antihypertensive agent.

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来源期刊
Animal Bioscience
Animal Bioscience AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
5.00
自引率
0.00%
发文量
223
审稿时长
3 months
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