揭示新型碳水化合物结合模块在浮游植物大量繁殖期间海洋类杆菌多模块蛋白与层叠蛋白相互作用中的作用

IF 4.3 2区 生物学 Q2 MICROBIOLOGY
Marie-Katherin Zühlke, Elizabeth Ficko-Blean, Daniel Bartosik, Nicolas Terrapon, Alexandra Jeudy, Murielle Jam, Fengqing Wang, Norma Welsch, Alexandra Dürwald, Laura Torres Martin, Robert Larocque, Diane Jouanneau, Tom Eisenack, François Thomas, Anke Trautwein-Schult, Hanno Teeling, Dörte Becher, Thomas Schweder, Mirjam Czjzek
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引用次数: 0

摘要

层藻糖苷是一种β(1,3)-葡聚糖,是硅藻等海洋微藻的一种贮藏多糖。其丰富的数量、水溶性和简单的结构使其成为海洋细菌喜爱的底物。因此,许多海洋细菌利用碳水化合物结合模块(CBMs)作为关键部件,进化出了清除和分解层聚糖的策略。在这项研究中,我们在海洋细菌 Christiangramia forsetii KT0803T 的多模块蛋白中鉴定了两个以前未分配的结构域作为层粘蛋白结合 CBM,从而引入了新的层粘蛋白结合 CBM 家族 CBM102 和 CBM103。我们在一个表面糖蛋白结合蛋白(SGBP)中发现了四个 CBM102,并从第 16 家族(GH16_3)中发现了一个与糖苷水解酶模块相连的 CBM103。我们的分析表明,这两种模块蛋白都具有拉长的形状,其中 GH16_3 比 SGBP 表现出更大的灵活性。这种灵活性可能有助于识别和/或降解层粘菌素,而 SGBP 的限制则有助于层粘菌素与细菌表面的对接。对来自北海浮游植物藻华的细菌元基因组(MAGs)的探索表明,这两种结合层粘菌素的 CBM 家族在海洋类杆菌中都很普遍。在浮游植物藻华期间,含 CBM102 和 CBM103 蛋白的蛋白质丰度很高,这进一步强调了它们在海洋层皮素利用中的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Unveiling the role of novel carbohydrate-binding modules in laminarin interaction of multimodular proteins from marine Bacteroidota during phytoplankton blooms

Unveiling the role of novel carbohydrate-binding modules in laminarin interaction of multimodular proteins from marine Bacteroidota during phytoplankton blooms

Laminarin, a β(1,3)-glucan, serves as a storage polysaccharide in marine microalgae such as diatoms. Its abundance, water solubility and simple structure make it an appealing substrate for marine bacteria. Consequently, many marine bacteria have evolved strategies to scavenge and decompose laminarin, employing carbohydrate-binding modules (CBMs) as crucial components. In this study, we characterized two previously unassigned domains as laminarin-binding CBMs in multimodular proteins from the marine bacterium Christiangramia forsetii KT0803T, thereby introducing the new laminarin-binding CBM families CBM102 and CBM103. We identified four CBM102s in a surface glycan-binding protein (SGBP) and a single CBM103 linked to a glycoside hydrolase module from family 16 (GH16_3). Our analysis revealed that both modular proteins have an elongated shape, with GH16_3 exhibiting greater flexibility than SGBP. This flexibility may aid in the recognition and/or degradation of laminarin, while the constraints in SGBP could facilitate the docking of laminarin onto the bacterial surface. Exploration of bacterial metagenome-assembled genomes (MAGs) from phytoplankton blooms in the North Sea showed that both laminarin-binding CBM families are widespread among marine Bacteroidota. The high protein abundance of CBM102- and CBM103-containing proteins during phytoplankton blooms further emphasizes their significance in marine laminarin utilization.

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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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