迷迭香酸对诱发多形性胶质母细胞瘤动物模型中细胞增殖、氧化应激和凋亡途径的影响

IF 4.7 3区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Sepideh Khaksar , Khadijeh Kiarostami , Mahmoud Ramdan
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引用次数: 0

摘要

背景在脑肿瘤中,氧化应激、细胞增殖、血管生成和细胞凋亡等病理生理过程的复杂性对确定性治疗提出了严峻挑战。迷迭香酸(RA)作为一种多酚类化合物,已被发现能阻止一些侵袭性癌症的肿瘤进展。本研究旨在评估迷迭香酸对脑肿瘤的抗癌作用。将 C6 脑胶质瘤细胞植入右半球尾状核。给治疗组大鼠注射 5、10 和 20 毫克/千克剂量的 RA,连续注射 7 天。对肿瘤体积(通过核磁共振成像)、运动能力、存活时间、组织学改变(通过H& E染色)、p53和p21 mRNA的表达(通过RT-PCR)、抗氧化酶的活性(通过检测试剂盒检测超氧化物歧化酶[SOD]和过氧化氢酶[CAT])、caspase-3和VEGF的表达(通过免疫组化分析)以及TUNEL阳性细胞(通过隧道染色)进行了分析。结果表明,20 毫克/千克剂量的 RA 可减少肿瘤体积,延长存活时间,增加 p53 和 p21 mRNA,降低肿瘤组织中 SOD 和 CAT 的活性,升高 caspase-3,增加 TUNEL 阳性细胞的数量。此外,组织学分析表明,接受 RA(20 毫克/千克)治疗的大鼠肿瘤细胞侵入正常实质组织的情况较少。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Rosmarinic Acid on Cell Proliferation, Oxidative Stress, and Apoptosis Pathways in an Animal Model of Induced Glioblastoma Multiforme

Background

In brain tumors, the complexity of the pathophysiological processes such as oxidative stress, cell proliferation, angiogenesis, and apoptosis have seriously challenged the definitive treatment. Rosmarinic acid (RA), as a polyphenolic compound, has been found to prevent tumor progression in some aggressive cancers. This study was designed to evaluate the anticancer effects of RA on brain tumors.

Method

Rats were divided into six groups. Implantation of C6 glioma cells was carried out in the caudate nucleus of the right hemisphere. RA at doses of 5, 10, and 20 mg/kg (i.p.) was administered to the treatment groups for seven days. Tumor volume (by MRI imaging), locomotor ability, survival time, histological alterations (by H & E staining), expression of p53 and p21 mRNAs (by RT-PCR), activities of antioxidant enzymes (superoxide dismutase [SOD] and catalase [CAT] by assay kits), expression of caspase-3 and VEGF (by immunohistochemical analysis), and TUNEL-positive cells (by tunnel staining) were analyzed.

Results

The results indicated that the RA at a dose of 20 mg/kg reduced the tumor volume, prolonged survival time, increased p53 and p21 mRNAs, attenuated SOD and CAT activities in tumor tissue, elevated caspase-3, and increased the number of TUNEL-positive cells. Furthermore, histological analysis revealed less invasion of tumor cells into the normal parenchyma in rats treated with RA (20 mg/kg).

Conclusion

These findings provide evidence that the ability of RA to reduce tumor volume could be related to factors that modulate oxidative stress (SOD and CAT enzymes), cell proliferation (p53 and p21), and apoptosis (caspase-3).

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来源期刊
Archives of Medical Research
Archives of Medical Research 医学-医学:研究与实验
CiteScore
12.50
自引率
0.00%
发文量
84
审稿时长
28 days
期刊介绍: Archives of Medical Research serves as a platform for publishing original peer-reviewed medical research, aiming to bridge gaps created by medical specialization. The journal covers three main categories - biomedical, clinical, and epidemiological contributions, along with review articles and preliminary communications. With an international scope, it presents the study of diseases from diverse perspectives, offering the medical community original investigations ranging from molecular biology to clinical epidemiology in a single publication.
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