Dimitry Y. Sorokin , Alexander Y. Merkel , Tatyana V. Kolganova , Nicole J. Bale , Jaap S. Sinninghe Damsté
{"title":"Natronospira bacteriovora sp.nov.和 Natronospira elongata sp.nov.--来自苏打湖的极耐盐性掠夺性蛋白水解细菌，并建议将 Natronospira 属归入 Natronospiraceae fam.","authors":"Dimitry Y. Sorokin , Alexander Y. Merkel , Tatyana V. Kolganova , Nicole J. Bale , Jaap S. Sinninghe Damsté","doi":"10.1016/j.syapm.2024.126519","DOIUrl":null,"url":null,"abstract":"<div><p>The genus <em>Natronospira</em> is represented by a single species of extremely salt-tolerant aerobic alkaliphilic proteolytic bacterium, isolated from hypersaline soda lakes. When cells of Gram-positive cocci were used as a substrate instead of proteins at extremely haloalkaline conditions, two new members of this genus were enriched and isolated in pure culture from the same sites. Strains AB-CW1 and AB-CW4 are obligate aerobic heterotrophic proteolytic bacteria able to feed on both live and dead cells of staphylococci and a range of proteins and peptides. Similar to the type species, <em>N. proteinivora</em>, the isolates are extremely salt-tolerant obligate alkaliphiles. However, <em>N. proteinivora</em> was unable to use bacterial cells as a substrate. Electron microscopy showed direct contact between the prey and predator cells. Functional analysis of the AB-CW1 and AB-CW4 genomes identified two sets of genes coding for extracellular enzymes potentially involved in the predation and proteolysis, respectively. The first set includes several copies of lysozyme-like GH23 peptidoglycan-lyase and murein-specific M23 [Zn]-di-peptidase enabling the cell wall degradation. The second set features multiple copies of secreted serine and metallopeptidases apparently allowing for the strong proteolytic phenotype. Phylogenomic analysis placed the isolates into the genus <em>Natronospira</em> as two novel species members, and furthermore indicated that this genus forms a deep-branching lineage of a new family (<em>Natronospiraceae</em>) and order (<em>Natronospirales</em>) within the class <em>Gammaproteobacteria.</em> On the basis of distinct phenotypic and genomic properties, strain AB-CW1<sup>T</sup> (JCM 335396 = UQM 41579) is proposed to be classified as <em>Natronospira elongata</em> sp. nov., and AB-CW4<sup>T</sup> (JCM 335397 = UQM 41580) as <em>Natronospira bacteriovora</em> sp. nov.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"47 4","pages":"Article 126519"},"PeriodicalIF":3.3000,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S072320202400033X/pdfft?md5=9ad22332e74e292a8c610f955924194f&pid=1-s2.0-S072320202400033X-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Natronospira bacteriovora sp. nov., and Natronospira elongata sp. nov., extremely salt-tolerant predatory proteolytic bacteria from soda lakes and proposal to classify the genus Natronospira into Natronospiraceae fam. nov., and Natronospirales ord. nov., within the class Gammaproteobacteria\",\"authors\":\"Dimitry Y. Sorokin , Alexander Y. Merkel , Tatyana V. Kolganova , Nicole J. Bale , Jaap S. Sinninghe Damsté\",\"doi\":\"10.1016/j.syapm.2024.126519\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The genus <em>Natronospira</em> is represented by a single species of extremely salt-tolerant aerobic alkaliphilic proteolytic bacterium, isolated from hypersaline soda lakes. When cells of Gram-positive cocci were used as a substrate instead of proteins at extremely haloalkaline conditions, two new members of this genus were enriched and isolated in pure culture from the same sites. Strains AB-CW1 and AB-CW4 are obligate aerobic heterotrophic proteolytic bacteria able to feed on both live and dead cells of staphylococci and a range of proteins and peptides. Similar to the type species, <em>N. proteinivora</em>, the isolates are extremely salt-tolerant obligate alkaliphiles. However, <em>N. proteinivora</em> was unable to use bacterial cells as a substrate. Electron microscopy showed direct contact between the prey and predator cells. Functional analysis of the AB-CW1 and AB-CW4 genomes identified two sets of genes coding for extracellular enzymes potentially involved in the predation and proteolysis, respectively. The first set includes several copies of lysozyme-like GH23 peptidoglycan-lyase and murein-specific M23 [Zn]-di-peptidase enabling the cell wall degradation. The second set features multiple copies of secreted serine and metallopeptidases apparently allowing for the strong proteolytic phenotype. Phylogenomic analysis placed the isolates into the genus <em>Natronospira</em> as two novel species members, and furthermore indicated that this genus forms a deep-branching lineage of a new family (<em>Natronospiraceae</em>) and order (<em>Natronospirales</em>) within the class <em>Gammaproteobacteria.</em> On the basis of distinct phenotypic and genomic properties, strain AB-CW1<sup>T</sup> (JCM 335396 = UQM 41579) is proposed to be classified as <em>Natronospira elongata</em> sp. nov., and AB-CW4<sup>T</sup> (JCM 335397 = UQM 41580) as <em>Natronospira bacteriovora</em> sp. nov.</p></div>\",\"PeriodicalId\":22124,\"journal\":{\"name\":\"Systematic and applied microbiology\",\"volume\":\"47 4\",\"pages\":\"Article 126519\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-05-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S072320202400033X/pdfft?md5=9ad22332e74e292a8c610f955924194f&pid=1-s2.0-S072320202400033X-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Systematic and applied microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S072320202400033X\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Systematic and applied microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S072320202400033X","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Natronospira bacteriovora sp. nov., and Natronospira elongata sp. nov., extremely salt-tolerant predatory proteolytic bacteria from soda lakes and proposal to classify the genus Natronospira into Natronospiraceae fam. nov., and Natronospirales ord. nov., within the class Gammaproteobacteria
The genus Natronospira is represented by a single species of extremely salt-tolerant aerobic alkaliphilic proteolytic bacterium, isolated from hypersaline soda lakes. When cells of Gram-positive cocci were used as a substrate instead of proteins at extremely haloalkaline conditions, two new members of this genus were enriched and isolated in pure culture from the same sites. Strains AB-CW1 and AB-CW4 are obligate aerobic heterotrophic proteolytic bacteria able to feed on both live and dead cells of staphylococci and a range of proteins and peptides. Similar to the type species, N. proteinivora, the isolates are extremely salt-tolerant obligate alkaliphiles. However, N. proteinivora was unable to use bacterial cells as a substrate. Electron microscopy showed direct contact between the prey and predator cells. Functional analysis of the AB-CW1 and AB-CW4 genomes identified two sets of genes coding for extracellular enzymes potentially involved in the predation and proteolysis, respectively. The first set includes several copies of lysozyme-like GH23 peptidoglycan-lyase and murein-specific M23 [Zn]-di-peptidase enabling the cell wall degradation. The second set features multiple copies of secreted serine and metallopeptidases apparently allowing for the strong proteolytic phenotype. Phylogenomic analysis placed the isolates into the genus Natronospira as two novel species members, and furthermore indicated that this genus forms a deep-branching lineage of a new family (Natronospiraceae) and order (Natronospirales) within the class Gammaproteobacteria. On the basis of distinct phenotypic and genomic properties, strain AB-CW1T (JCM 335396 = UQM 41579) is proposed to be classified as Natronospira elongata sp. nov., and AB-CW4T (JCM 335397 = UQM 41580) as Natronospira bacteriovora sp. nov.
期刊介绍：
Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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