溃疡性结肠炎患者的巨噬细胞免疫抑制功能受损

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Baisui Feng , Jin Liu , Jia Li , Shiyu Feng , Lingzhi Xu , Xiangyu Wang , Shuo Song , Yan Li , Aifa Tang , Yu Liu , Qing Shu , Pingchang Yang
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引用次数: 0

摘要

慢性炎症是炎症性肠病(IBD)的病理特征,但其病因尚不清楚。巨噬细胞是结肠中主要的免疫细胞组分之一。本研究的目的是描述溃疡性结肠炎(UC)患者结肠中巨噬细胞的免疫调节功能。本研究招募了溃疡性结肠炎患者(n = 30)。收集结肠灌洗液(CLF)。从结肠灌洗液的细胞成分中分离出巨噬细胞。使用免疫学方法评估了巨噬细胞的免疫抑制功能。我们观察到,巨噬细胞约占 CLF 中细胞成分比例的一半。在 UC 组的巨噬细胞中检测到的 IL10 mRNA 和蛋白量低于正常对照组(NC)。IL10在CLF巨噬细胞中的表达与UC相关细胞因子(包括肿瘤坏死因子-α、白细胞介素(IL)-1β、IFN-γ、嗜酸性粒细胞衍生介质)在CLF中的表达呈正相关。UC 患者 CLF 巨噬细胞的免疫抑制功能受损。与NC M0细胞相比,UC M0细胞表达IL10的诱导性存在缺陷。暴露于CpG可恢复UC M0细胞IL10表达的诱导性,并获得获得免疫抑制功能的潜力。综上所述,UC 巨噬细胞的免疫抑制功能受损。M0细胞IL10表达的诱导性受损,而CpG可恢复其表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The immune suppressive functions of macrophages are impaired in patients with ulcerative colitis

Chronic inflammation is the pathological feature of inflammatory bowel diseases (IBD), but its etiology is unknown. Macrophages are one of the major immune cell fractions in the colon. The objectives of this study are to characterize the immune regulatory functions of macrophages in the colon of patients with ulcerative colitis (UC). UC patients (n = 30) were recruited into this study. Colon lavage fluid (CLF) was collected. Macrophages are isolated from the cellular components of CLF. The immune suppressive functions of macrophages were assessed using immunological approaches. We observed that macrophages occupied about half of the proportions of the cellular components in CLF. Lower amounts of IL10 mRNA and proteins were detected in macrophages of the UC group than the normal control (NC) group. The expression of IL10 in CLF macrophages was positively correlated with the UC-associated cytokines, including tumor necrosis factor-α, interleukin (IL)-1β, IFN-γ, eosinophil-derived mediators, in CLF. The immune suppressive functions of CLF macrophages in UC patients were impaired. The inducibility of IL10 expression of UC M0 cells was defective as compared with NC M0 cells. Exposure to CpG restored the inducibility of IL10 expression in UC M0 cells, and gain the potential to acquire the immune suppressive functions. To sum up, the immune suppressive functions of UC macrophages are impaired. The inducibility of IL10 expression of M0 cells is impaired, which can be restored by the treatment with CpG.

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CiteScore
7.20
自引率
4.30%
发文量
567
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