Jaclyn Dietrich, Tessa E. LeCuyer, G. Kenitra Hendrix, Claire R. Burbick, Megan E. Jacob, Barbara A. Byrne, Karen Olsen, Maria Mitchell, Olgica Ceric, Rachel Lin, Jessica Joneson, Megan Lintner, Alexandra Fox, Diamond McClendon, Trevor Alexander, Kayla Joyce, Melissa Byrd, Jason Clinton, Keith Snipes, Laura Peak, Stephen D. Cole
{"title":"从提交给美国兽医实验室的狗和猫粪便中分离出的产碳青霉烯酶肠杆菌的流行率和分子流行病学。","authors":"Jaclyn Dietrich, Tessa E. LeCuyer, G. Kenitra Hendrix, Claire R. Burbick, Megan E. Jacob, Barbara A. Byrne, Karen Olsen, Maria Mitchell, Olgica Ceric, Rachel Lin, Jessica Joneson, Megan Lintner, Alexandra Fox, Diamond McClendon, Trevor Alexander, Kayla Joyce, Melissa Byrd, Jason Clinton, Keith Snipes, Laura Peak, Stephen D. Cole","doi":"10.1111/zph.13144","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Aims</h3>\n \n <p>To estimate the prevalence of carbapenemase-producing Enterobacterales (CPE) carriage among pets using faecal specimens submitted to veterinary diagnostic laboratories throughout the US. A secondary aim was to employ whole-genome sequencing (WGS) to characterize isolates of CPE from companion animals and compare them to publicly available CPE genomes.</p>\n </section>\n \n <section>\n \n <h3> Methods and Results</h3>\n \n <p>To estimate the prevalence of CPE in companion animals in the USA, a multicenter surveillance study including 8 different veterinary diagnostic laboratories from across the USA was conducted. Briefly, remnant faecal specimens from dogs and cats were screened using two selective agar plates (CHROMID Carba and MacConkey with 1 mg/L cefotaxime and 0.125 mg/L meropenem) and presumptive CPE isolates screened by the modified carbapenemase inactivation method for carbapenemase production. A total of 2393 specimens were screened and yielded 196 isolates for carbapenemase screening. A total of 5 isolates from 4 dogs and 1 cat at 3 different veterinary diagnostic laboratories were confirmed to produce a carbapenemase (0.21%). Whole-genome sequencing (WGS) revealed two <i>E. coli</i> (ST167) isolates that both produced an NDM-5 carbapenemase, two <i>Enterobacter hormaechei</i> (ST171) isolates that produced an NDM-5 carbapenemase and a KPC-4 carbapenemase respectively and one <i>Klebsiella oxytoca</i> (ST199) that produced an Oxa-48-type carbapenemase. Both <i>E. coli</i> isolates were found to be within at least 22 SNPs of previously characterized canine and human CPE isolates.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>This study demonstrates that the prevalence of CPE among companion animals is relatively low (0.21%) but that given the genetic relatedness of animal isolates to human isolates, additional surveillance is needed.</p>\n </section>\n </div>","PeriodicalId":24025,"journal":{"name":"Zoonoses and Public Health","volume":"71 5","pages":"538-548"},"PeriodicalIF":2.3000,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/zph.13144","citationCount":"0","resultStr":"{\"title\":\"Prevalence and molecular epidemiology of carbapenemase-producing Enterobacterales isolated from dog and cat faeces submitted to veterinary laboratories in the USA\",\"authors\":\"Jaclyn Dietrich, Tessa E. LeCuyer, G. Kenitra Hendrix, Claire R. Burbick, Megan E. Jacob, Barbara A. Byrne, Karen Olsen, Maria Mitchell, Olgica Ceric, Rachel Lin, Jessica Joneson, Megan Lintner, Alexandra Fox, Diamond McClendon, Trevor Alexander, Kayla Joyce, Melissa Byrd, Jason Clinton, Keith Snipes, Laura Peak, Stephen D. Cole\",\"doi\":\"10.1111/zph.13144\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Aims</h3>\\n \\n <p>To estimate the prevalence of carbapenemase-producing Enterobacterales (CPE) carriage among pets using faecal specimens submitted to veterinary diagnostic laboratories throughout the US. A secondary aim was to employ whole-genome sequencing (WGS) to characterize isolates of CPE from companion animals and compare them to publicly available CPE genomes.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods and Results</h3>\\n \\n <p>To estimate the prevalence of CPE in companion animals in the USA, a multicenter surveillance study including 8 different veterinary diagnostic laboratories from across the USA was conducted. Briefly, remnant faecal specimens from dogs and cats were screened using two selective agar plates (CHROMID Carba and MacConkey with 1 mg/L cefotaxime and 0.125 mg/L meropenem) and presumptive CPE isolates screened by the modified carbapenemase inactivation method for carbapenemase production. A total of 2393 specimens were screened and yielded 196 isolates for carbapenemase screening. A total of 5 isolates from 4 dogs and 1 cat at 3 different veterinary diagnostic laboratories were confirmed to produce a carbapenemase (0.21%). Whole-genome sequencing (WGS) revealed two <i>E. coli</i> (ST167) isolates that both produced an NDM-5 carbapenemase, two <i>Enterobacter hormaechei</i> (ST171) isolates that produced an NDM-5 carbapenemase and a KPC-4 carbapenemase respectively and one <i>Klebsiella oxytoca</i> (ST199) that produced an Oxa-48-type carbapenemase. Both <i>E. coli</i> isolates were found to be within at least 22 SNPs of previously characterized canine and human CPE isolates.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>This study demonstrates that the prevalence of CPE among companion animals is relatively low (0.21%) but that given the genetic relatedness of animal isolates to human isolates, additional surveillance is needed.</p>\\n </section>\\n </div>\",\"PeriodicalId\":24025,\"journal\":{\"name\":\"Zoonoses and Public Health\",\"volume\":\"71 5\",\"pages\":\"538-548\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-05-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/zph.13144\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zoonoses and Public Health\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/zph.13144\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zoonoses and Public Health","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/zph.13144","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Prevalence and molecular epidemiology of carbapenemase-producing Enterobacterales isolated from dog and cat faeces submitted to veterinary laboratories in the USA
Aims
To estimate the prevalence of carbapenemase-producing Enterobacterales (CPE) carriage among pets using faecal specimens submitted to veterinary diagnostic laboratories throughout the US. A secondary aim was to employ whole-genome sequencing (WGS) to characterize isolates of CPE from companion animals and compare them to publicly available CPE genomes.
Methods and Results
To estimate the prevalence of CPE in companion animals in the USA, a multicenter surveillance study including 8 different veterinary diagnostic laboratories from across the USA was conducted. Briefly, remnant faecal specimens from dogs and cats were screened using two selective agar plates (CHROMID Carba and MacConkey with 1 mg/L cefotaxime and 0.125 mg/L meropenem) and presumptive CPE isolates screened by the modified carbapenemase inactivation method for carbapenemase production. A total of 2393 specimens were screened and yielded 196 isolates for carbapenemase screening. A total of 5 isolates from 4 dogs and 1 cat at 3 different veterinary diagnostic laboratories were confirmed to produce a carbapenemase (0.21%). Whole-genome sequencing (WGS) revealed two E. coli (ST167) isolates that both produced an NDM-5 carbapenemase, two Enterobacter hormaechei (ST171) isolates that produced an NDM-5 carbapenemase and a KPC-4 carbapenemase respectively and one Klebsiella oxytoca (ST199) that produced an Oxa-48-type carbapenemase. Both E. coli isolates were found to be within at least 22 SNPs of previously characterized canine and human CPE isolates.
Conclusions
This study demonstrates that the prevalence of CPE among companion animals is relatively low (0.21%) but that given the genetic relatedness of animal isolates to human isolates, additional surveillance is needed.
期刊介绍：
Zoonoses and Public Health brings together veterinary and human health researchers and policy-makers by providing a venue for publishing integrated and global approaches to zoonoses and public health. The Editors will consider papers that focus on timely collaborative and multi-disciplinary research in zoonoses and public health. This journal provides rapid publication of original papers, reviews, and potential discussion papers embracing this collaborative spirit. Papers should advance the scientific knowledge of the sources, transmission, prevention and control of zoonoses and be authored by scientists with expertise in areas such as microbiology, virology, parasitology and epidemiology. Articles that incorporate recent data into new methods, applications, or approaches (e.g. statistical modeling) which enhance public health are strongly encouraged.
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