{"title":"短暂接触溶菌酶和乳铁蛋白对口腔念珠菌致病特性的影响","authors":"","doi":"10.1016/j.identj.2024.04.003","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction and aims</h3><p>Adhesion to buccal epithelial cells (BEC) and denture acrylic surfaces (DAS), germ tube (GT) formation, cell surface hydrophobicity (CSH), and haemolysin production are attributes associated with pathogenicity of <em>Candida. Candida albicans</em> and <em>Candida dubliniensis</em> are allied in causing oral candidosis. Lysozyme and lactoferrin exert antimicrobial activity on a range of oral microorganisms, including <em>Candida</em>. There is no information on the impact of brief exposure to lysozyme and lactoferrin on adhesion-related attributes and haemolysin production of aforementioned oral <em>Candida</em> isolates. Thus, we investigated the impact of lysozyme and lactoferrin on adhesion to BEC and DAS, GT formation, CSH, and haemolysin production of these isolates.</p></div><div><h3>Methods</h3><p>After exposure to lysozyme and lactoferrin for 1 hour, susceptibility to lysozyme and lactoferrin of 20 isolates each of <em>C albicans</em> and <em>C dubliniensis</em> isolates was determined following a 48-hour period of incubation. <em>Candida</em> cell suspensions, obtained from colony-forming units after this period, were assessed for adhesion to BEC and DAS, GT formation, CSH, and haemolysin production using <em>in vitro</em> assays.</p></div><div><h3>Results</h3><p>Exposure to lysozyme and lactoferrin significantly suppressed the ability of <em>C albicans</em> and <em>C dubliniensis</em> isolates to adhere to BEC and DAS, GT formation, CSH, and haemolysin production (<em>P</em> < 0.01 for all virulent attributes tested).</p></div><div><h3>Conclusions</h3><p>These data provide a tantalising glimpse into the possibility that exposure to either lysozyme or lactoferrin, even for a brief period, would induce a sustainable antifungal effect by suppressing adhesion-related attributes and haemolysin production of these oral <em>Candida</em> species <em>in vitro</em>. Resistance to conventional antifungal agents has been reported in clinical isolates of <em>Candida</em>. The presence of such resistance indicates the need for possible alternative therapies to facilitate the management of oral candidosis. Further research on the pharmacodynamics of lysozyme and lactoferrin and their effects on candidal pathogenic attributes should be fostered, with the vision of developing novel topical antifungal drugs.</p></div>","PeriodicalId":13785,"journal":{"name":"International dental journal","volume":"74 5","pages":"Pages 1161-1167"},"PeriodicalIF":3.2000,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0020653924001096/pdfft?md5=ff0078575f9bd7f117ba311867b4972f&pid=1-s2.0-S0020653924001096-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Impact of Brief Exposure to Lysozyme and Lactoferrin on Pathogenic Attributes of Oral Candida\",\"authors\":\"\",\"doi\":\"10.1016/j.identj.2024.04.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction and aims</h3><p>Adhesion to buccal epithelial cells (BEC) and denture acrylic surfaces (DAS), germ tube (GT) formation, cell surface hydrophobicity (CSH), and haemolysin production are attributes associated with pathogenicity of <em>Candida. Candida albicans</em> and <em>Candida dubliniensis</em> are allied in causing oral candidosis. Lysozyme and lactoferrin exert antimicrobial activity on a range of oral microorganisms, including <em>Candida</em>. There is no information on the impact of brief exposure to lysozyme and lactoferrin on adhesion-related attributes and haemolysin production of aforementioned oral <em>Candida</em> isolates. Thus, we investigated the impact of lysozyme and lactoferrin on adhesion to BEC and DAS, GT formation, CSH, and haemolysin production of these isolates.</p></div><div><h3>Methods</h3><p>After exposure to lysozyme and lactoferrin for 1 hour, susceptibility to lysozyme and lactoferrin of 20 isolates each of <em>C albicans</em> and <em>C dubliniensis</em> isolates was determined following a 48-hour period of incubation. <em>Candida</em> cell suspensions, obtained from colony-forming units after this period, were assessed for adhesion to BEC and DAS, GT formation, CSH, and haemolysin production using <em>in vitro</em> assays.</p></div><div><h3>Results</h3><p>Exposure to lysozyme and lactoferrin significantly suppressed the ability of <em>C albicans</em> and <em>C dubliniensis</em> isolates to adhere to BEC and DAS, GT formation, CSH, and haemolysin production (<em>P</em> < 0.01 for all virulent attributes tested).</p></div><div><h3>Conclusions</h3><p>These data provide a tantalising glimpse into the possibility that exposure to either lysozyme or lactoferrin, even for a brief period, would induce a sustainable antifungal effect by suppressing adhesion-related attributes and haemolysin production of these oral <em>Candida</em> species <em>in vitro</em>. Resistance to conventional antifungal agents has been reported in clinical isolates of <em>Candida</em>. The presence of such resistance indicates the need for possible alternative therapies to facilitate the management of oral candidosis. Further research on the pharmacodynamics of lysozyme and lactoferrin and their effects on candidal pathogenic attributes should be fostered, with the vision of developing novel topical antifungal drugs.</p></div>\",\"PeriodicalId\":13785,\"journal\":{\"name\":\"International dental journal\",\"volume\":\"74 5\",\"pages\":\"Pages 1161-1167\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2024-05-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S0020653924001096/pdfft?md5=ff0078575f9bd7f117ba311867b4972f&pid=1-s2.0-S0020653924001096-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International dental journal\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0020653924001096\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International dental journal","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0020653924001096","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Impact of Brief Exposure to Lysozyme and Lactoferrin on Pathogenic Attributes of Oral Candida
Introduction and aims
Adhesion to buccal epithelial cells (BEC) and denture acrylic surfaces (DAS), germ tube (GT) formation, cell surface hydrophobicity (CSH), and haemolysin production are attributes associated with pathogenicity of Candida. Candida albicans and Candida dubliniensis are allied in causing oral candidosis. Lysozyme and lactoferrin exert antimicrobial activity on a range of oral microorganisms, including Candida. There is no information on the impact of brief exposure to lysozyme and lactoferrin on adhesion-related attributes and haemolysin production of aforementioned oral Candida isolates. Thus, we investigated the impact of lysozyme and lactoferrin on adhesion to BEC and DAS, GT formation, CSH, and haemolysin production of these isolates.
Methods
After exposure to lysozyme and lactoferrin for 1 hour, susceptibility to lysozyme and lactoferrin of 20 isolates each of C albicans and C dubliniensis isolates was determined following a 48-hour period of incubation. Candida cell suspensions, obtained from colony-forming units after this period, were assessed for adhesion to BEC and DAS, GT formation, CSH, and haemolysin production using in vitro assays.
Results
Exposure to lysozyme and lactoferrin significantly suppressed the ability of C albicans and C dubliniensis isolates to adhere to BEC and DAS, GT formation, CSH, and haemolysin production (P < 0.01 for all virulent attributes tested).
Conclusions
These data provide a tantalising glimpse into the possibility that exposure to either lysozyme or lactoferrin, even for a brief period, would induce a sustainable antifungal effect by suppressing adhesion-related attributes and haemolysin production of these oral Candida species in vitro. Resistance to conventional antifungal agents has been reported in clinical isolates of Candida. The presence of such resistance indicates the need for possible alternative therapies to facilitate the management of oral candidosis. Further research on the pharmacodynamics of lysozyme and lactoferrin and their effects on candidal pathogenic attributes should be fostered, with the vision of developing novel topical antifungal drugs.
期刊介绍:
The International Dental Journal features peer-reviewed, scientific articles relevant to international oral health issues, as well as practical, informative articles aimed at clinicians.