通过单泵光同步振荡器和光谱聚焦实现的跨振动光谱双相干反斯托克斯拉曼散射显微技术

IF 2.4 3区 化学 Q2 SPECTROSCOPY
Dominykas Gudavičius, Lukas Kontenis, Wolfgang Langbein
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引用次数: 0

摘要

演示了相干反斯托克斯拉曼散射显微镜,可探测整个振动光谱中两个独立可选的振动频率。一个单箱飞秒激光源提供三个同步的 100-200 fs 脉冲,重复频率为 77 MHz,其中一个来自掺镱振荡器,波长固定为 1025 nm,另两个来自两个光学同步参量振荡器,波长分别为 680-960 nm 和 960-1300 nm。结合光谱聚焦,该光源可同时处理指纹、细胞沉默和 C-H 伸展振动区域,避免了顺序成像中出现的运动伪影。我们在细胞内的水/重水/油乳液、塑料珠和氘化脂质上演示了这种显微镜方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Dual coherent anti-Stokes Raman scattering microscopy across the vibrational spectrum enabled by single pump optically synchronised oscillators and spectral focusing

Dual coherent anti-Stokes Raman scattering microscopy across the vibrational spectrum enabled by single pump optically synchronised oscillators and spectral focusing

Coherent anti-Stokes Raman scattering microscopy probing two independently selectable vibrational frequencies across the vibrational spectrum is demonstrated. A one-box femtosecond laser source provides three synchronised 100–200 fs pulses of 77 MHz repetition rate, one at a fixed wavelength of 1025 nm from an Yb oscillator and two independently tuneable ones, over 680–960 nm and 960–1300 nm, respectively, from two optically synchronised parametric oscillators. Combined with spectral focusing, the source allows addressing the fingerprint, cell silent and C–H stretch vibrational regions simultaneously, avoiding the motion artefacts seen in sequential imaging. We demonstrate the microscopy method on water/heavy water/oil emulsions, plastic beads and deuterated lipids inside cells.

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来源期刊
CiteScore
5.40
自引率
8.00%
发文量
185
审稿时长
3.0 months
期刊介绍: The Journal of Raman Spectroscopy is an international journal dedicated to the publication of original research at the cutting edge of all areas of science and technology related to Raman spectroscopy. The journal seeks to be the central forum for documenting the evolution of the broadly-defined field of Raman spectroscopy that includes an increasing number of rapidly developing techniques and an ever-widening array of interdisciplinary applications. Such topics include time-resolved, coherent and non-linear Raman spectroscopies, nanostructure-based surface-enhanced and tip-enhanced Raman spectroscopies of molecules, resonance Raman to investigate the structure-function relationships and dynamics of biological molecules, linear and nonlinear Raman imaging and microscopy, biomedical applications of Raman, theoretical formalism and advances in quantum computational methodology of all forms of Raman scattering, Raman spectroscopy in archaeology and art, advances in remote Raman sensing and industrial applications, and Raman optical activity of all classes of chiral molecules.
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