{"title":"Aurantiochytrium sp.的生长动力学和脂质代谢：对其潜在应用的启示","authors":"Zahra Shafaghat, Farzaneh Najafi, Ramazan-Ali Khavari-Nejad, Mehdi Mohammadi, Sattar Tahmasebi Enferadi","doi":"10.1007/s10452-024-10105-6","DOIUrl":null,"url":null,"abstract":"<div><p><i>Aurantiochytrium</i> sp. is a marine microalga known for its bioactive compounds. This study assessed its presence in Iran and investigated its specific biochemical and molecular traits. Parameters such as biomass, protein content, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA) levels, catalase enzyme (CAT) activity, and expression of malic enzyme (ME) and acetyl-CoA carboxylase (ACCase) genes were analyzed at different growth stages (24, 48, 72, and 96 h). Fatty acid composition, unsaturated fatty acids (UFA) and saturated fatty acids (SFA) contents, and the ratio of UFA to SFA were also examined. Molecular identification confirmed successful proliferation, with significant increases in biomass and protein content during growth. Elevated H<sub>2</sub>O<sub>2</sub> and MDA levels indicated oxidative stress, whereas enhanced CAT activity suggested its potential role in scavenging reactive oxygen species and mitigating oxidative damage. The upregulation of <i>ME</i> and <i>ACCase</i> genes during growth indicated their involvement in lipid biosynthesis. This was further supported by the analysis of fatty acid composition, which revealed a higher proportion of UFA than SFA. These findings underscore the importance of specific growth stages for optimizing cultivation conditions and maximizing bioactive compound production in <i>Aurantiochytrium</i> sp. Overall, this study provides valuable insights into the growth dynamics and lipid metabolism of <i>Aurantiochytrium</i> sp., contributing to our understanding of its potential applications.</p></div>","PeriodicalId":8262,"journal":{"name":"Aquatic Ecology","volume":"58 3","pages":"789 - 799"},"PeriodicalIF":1.7000,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Growth dynamics and lipid metabolism of Aurantiochytrium sp.: insights into its potential applications\",\"authors\":\"Zahra Shafaghat, Farzaneh Najafi, Ramazan-Ali Khavari-Nejad, Mehdi Mohammadi, Sattar Tahmasebi Enferadi\",\"doi\":\"10.1007/s10452-024-10105-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><i>Aurantiochytrium</i> sp. is a marine microalga known for its bioactive compounds. This study assessed its presence in Iran and investigated its specific biochemical and molecular traits. Parameters such as biomass, protein content, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and malondialdehyde (MDA) levels, catalase enzyme (CAT) activity, and expression of malic enzyme (ME) and acetyl-CoA carboxylase (ACCase) genes were analyzed at different growth stages (24, 48, 72, and 96 h). Fatty acid composition, unsaturated fatty acids (UFA) and saturated fatty acids (SFA) contents, and the ratio of UFA to SFA were also examined. Molecular identification confirmed successful proliferation, with significant increases in biomass and protein content during growth. Elevated H<sub>2</sub>O<sub>2</sub> and MDA levels indicated oxidative stress, whereas enhanced CAT activity suggested its potential role in scavenging reactive oxygen species and mitigating oxidative damage. The upregulation of <i>ME</i> and <i>ACCase</i> genes during growth indicated their involvement in lipid biosynthesis. This was further supported by the analysis of fatty acid composition, which revealed a higher proportion of UFA than SFA. These findings underscore the importance of specific growth stages for optimizing cultivation conditions and maximizing bioactive compound production in <i>Aurantiochytrium</i> sp. Overall, this study provides valuable insights into the growth dynamics and lipid metabolism of <i>Aurantiochytrium</i> sp., contributing to our understanding of its potential applications.</p></div>\",\"PeriodicalId\":8262,\"journal\":{\"name\":\"Aquatic Ecology\",\"volume\":\"58 3\",\"pages\":\"789 - 799\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-05-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aquatic Ecology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10452-024-10105-6\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquatic Ecology","FirstCategoryId":"93","ListUrlMain":"https://link.springer.com/article/10.1007/s10452-024-10105-6","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ECOLOGY","Score":null,"Total":0}
Growth dynamics and lipid metabolism of Aurantiochytrium sp.: insights into its potential applications
Aurantiochytrium sp. is a marine microalga known for its bioactive compounds. This study assessed its presence in Iran and investigated its specific biochemical and molecular traits. Parameters such as biomass, protein content, hydrogen peroxide (H2O2) and malondialdehyde (MDA) levels, catalase enzyme (CAT) activity, and expression of malic enzyme (ME) and acetyl-CoA carboxylase (ACCase) genes were analyzed at different growth stages (24, 48, 72, and 96 h). Fatty acid composition, unsaturated fatty acids (UFA) and saturated fatty acids (SFA) contents, and the ratio of UFA to SFA were also examined. Molecular identification confirmed successful proliferation, with significant increases in biomass and protein content during growth. Elevated H2O2 and MDA levels indicated oxidative stress, whereas enhanced CAT activity suggested its potential role in scavenging reactive oxygen species and mitigating oxidative damage. The upregulation of ME and ACCase genes during growth indicated their involvement in lipid biosynthesis. This was further supported by the analysis of fatty acid composition, which revealed a higher proportion of UFA than SFA. These findings underscore the importance of specific growth stages for optimizing cultivation conditions and maximizing bioactive compound production in Aurantiochytrium sp. Overall, this study provides valuable insights into the growth dynamics and lipid metabolism of Aurantiochytrium sp., contributing to our understanding of its potential applications.
期刊介绍：
Aquatic Ecology publishes timely, peer-reviewed original papers relating to the ecology of fresh, brackish, estuarine and marine environments. Papers on fundamental and applied novel research in both the field and the laboratory, including descriptive or experimental studies, will be included in the journal. Preference will be given to studies that address timely and current topics and are integrative and critical in approach. We discourage papers that describe presence and abundance of aquatic biota in local habitats as well as papers that are pure systematic.
The journal provides a forum for the aquatic ecologist - limnologist and oceanologist alike- to discuss ecological issues related to processes and structures at different integration levels from individuals to populations, to communities and entire ecosystems.
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