Laura Roldan-Hernandez, Camila Van Oost and Alexandria B. Boehm
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Viral nucleic acids were extracted and quantified using reverse-transcription digital droplet PCR (RT-ddPCR). Partition coefficients (<em>K</em><small><sub>F</sub></small>), determined using the Freundlich adsorption model, ranged from 4.0 × 10<small><sup>2</sup></small> mL g<small><sup>−1</sup></small> to 3.9 × 10<small><sup>6</sup></small> mL g<small><sup>−1</sup></small> (median = 1.1 × 10<small><sup>4</sup></small> mL g<small><sup>−1</sup></small>). We applied a multiple linear regression model to evaluate the effects of factors like viruses and wastewater treatment plants on virus partitioning. We found that the individual effects of those variables were not significant, however, their combined effect was significant. Specifically, significant differences were observed between <em>K</em><small><sub>F</sub></small> for Zika and West Nile virus between wastewater treatment plants. Further research is needed to understand how wastewater characteristics might impact the partition of viral markers. The results from this experiment underscore the importance of considering wastewater solids for the early detection and monitoring of viral infectious diseases, particularly in regions with low prevalence of infections.</p>","PeriodicalId":75,"journal":{"name":"Environmental Science: Water Research & Technology","volume":" 1","pages":" 88-99"},"PeriodicalIF":3.5000,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2025/ew/d4ew00225c?page=search","citationCount":"0","resultStr":"{\"title\":\"Solid–liquid partitioning of dengue, West Nile, Zika, hepatitis A, influenza A, and SARS-CoV-2 viruses in wastewater from across the USA†\",\"authors\":\"Laura Roldan-Hernandez, Camila Van Oost and Alexandria B. 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引用次数: 0
摘要
有关呼吸道和节肢动物传播的病毒在废水中的去向的信息十分有限。对废水处理厂中的肠道病毒进行了广泛的研究,但对其分配系数却没有很好的记录。这些信息对于解释废水监测 (WBS) 数据以及优化样本采集和处理方法至关重要。在这项研究中,我们考察了登革热、西尼罗河病毒、寨卡病毒、甲型肝炎、甲型流感和 SARS-CoV-2 病毒在废水中的固液分配行为。样本从全美 11 家污水处理厂的一级污泥管线中收集,并添加了不同浓度的每种病毒。通过离心分离出固体和液体部分。采用反转录数字液滴 PCR(RT-ddPCR)技术提取病毒核酸并进行定量。利用弗伦德里希吸附模型确定的分配系数(KF)介于 4.0 × 102 mL g-1 至 3.9 × 106 mL g-1 之间(中位数 = 1.1 × 104 mL g-1)。我们采用多元线性回归模型来评估病毒和污水处理厂等因素对病毒分区的影响。我们发现,这些变量的单独影响并不显著,但它们的综合影响却很显著。具体来说,在不同污水处理厂之间,寨卡病毒和西尼罗河病毒的 KF 值存在明显差异。要了解废水特性如何影响病毒标记物的分区,还需要进一步的研究。本实验的结果强调了考虑废水固体对早期检测和监测病毒性传染病的重要性,尤其是在感染率较低的地区。
Solid–liquid partitioning of dengue, West Nile, Zika, hepatitis A, influenza A, and SARS-CoV-2 viruses in wastewater from across the USA†
Limited information is available on the fate of respiratory and arthropod-borne viruses in wastewater. Enteric viruses have been extensively studied in wastewater treatment plants, however partition coefficients have not been well documented. This information is essential for interpreting wastewater-based surveillance (WBS) data and optimizing sample collection and processing methods. In this study, we examined the solid–liquid partitioning behavior of dengue, West Nile, Zika, hepatitis A, influenza A, and SARS-CoV-2 viruses in wastewater. Samples were collected from the primary sludge line of eleven wastewater treatment plants across the United States and spiked with varying concentrations of each virus. Solid and liquid fractions were separated via centrifugation. Viral nucleic acids were extracted and quantified using reverse-transcription digital droplet PCR (RT-ddPCR). Partition coefficients (KF), determined using the Freundlich adsorption model, ranged from 4.0 × 102 mL g−1 to 3.9 × 106 mL g−1 (median = 1.1 × 104 mL g−1). We applied a multiple linear regression model to evaluate the effects of factors like viruses and wastewater treatment plants on virus partitioning. We found that the individual effects of those variables were not significant, however, their combined effect was significant. Specifically, significant differences were observed between KF for Zika and West Nile virus between wastewater treatment plants. Further research is needed to understand how wastewater characteristics might impact the partition of viral markers. The results from this experiment underscore the importance of considering wastewater solids for the early detection and monitoring of viral infectious diseases, particularly in regions with low prevalence of infections.
期刊介绍:
Environmental Science: Water Research & Technology seeks to showcase high quality research about fundamental science, innovative technologies, and management practices that promote sustainable water.