产前晚期小鼠血浆催乳素轴从胎盘向垂体转移

IF 1.3 4区 医学 Q4 ENDOCRINOLOGY & METABOLISM
Taku James Sairenji, Shinnosuke Masuda, Yuya Higuchi, Mitsue Miyazaki, Hiroyuki Yajima, Oh Kwan Ee, Yuki Fujiwara, Takuya Araki, Noriaki Shimokawa, Noriyuki Koibuchi
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引用次数: 0

摘要

胎盘分泌一种类似催乳素(PRL)的激素 PRL3B1(胎盘泌乳素 II),这是一种促黄体激素,对维持小鼠妊娠直至分娩至关重要。1984 年的一份报告研究了产前小鼠体内 PRL3B1 的分泌模式。在本次研究中,我们发现了分泌模式的矛盾之处,从而推翻了之前的报告。通过测量母体血浆PRL3B1和PRL,我们新发现从妊娠第17天(G17)开始,母体血浆PRL3B1水平在产前C57BL/6小鼠中迅速下降。有趣的是,这种下降的开始与 PRL 在 G18 的激增相吻合,这表明血浆催乳素轴从胎盘转移到了垂体。我们还发现,母体血浆孕酮的下降先于 PRL 转移的开始。通过 RT-qPCR 测定了胎盘中 Prl3b1 mRNA 的水平,该水平在分娩前一直保持稳定,这意味着 PRL3B1 肽的产生或分泌受到了抑制。我们假设,在此期间,PRL 家族(即专门在小鼠胎盘中表达的 25 个同族 PRL 蛋白)的产生将与 PRL3B1 一起减少。为了研究这一假设并寻找蛋白质组的变化,我们使用数据独立获取质谱(DIA-MS)对胎盘组织进行了霰弹枪蛋白质组分析。共鉴定出5891个蛋白质,其中包括17个PRL家族成员。胚胎第 17 天(E17)和 E18 胎盘之间的相对定量分析显示,除 PRL7C1 外,PRL3B1 和大多数 PRL 家族成员的表达量无显著差异。这些结果表明,胎盘中 PRL3B1 的分泌在 G18 (E18) 受到抑制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Plasma prolactin axis shift from placental to pituitary origin in late prepartum mice

The placenta secretes a prolactin (PRL)-like hormone PRL3B1 (placental lactogen II), a luteotropic hormone essential for maintaining pregnancy until labor in mice. A report from 1984 examined the secretion pattern of PRL3B1 in prepartum mice. In the current study, we found contradictory findings in the secretion pattern that invalidate the previous report. By measuring maternal plasma PRL3B1 and PRL every 4 hrs from gestational day 17 (G17), we newly discovered that maternal plasma PRL3B1 levels decrease rapidly in prepartum C57BL/6 mice. Interestingly, the onset of this decline coincided with the PRL surge at G18, demonstrating a plasma prolactin axis shift from placental to pituitary origin. We also found that maternal plasma progesterone regression precedes the onset of the PRL shift. The level of Prl3b1 mRNA was determined by RT-qPCR in the placenta and remained stable until parturition, implying that PRL3B1 peptide production or secretion was suppressed. We hypothesized that production of the PRL family, the 25 paralogous PRL proteins exclusively expressed in mice placenta, would decrease alongside PRL3B1 during this period. To investigate this hypothesis and to seek proteomic changes, we performed a shotgun proteome analysis of the placental tissue using data-independent acquisition mass spectrometry (DIA-MS). Up to 5,891 proteins were identified, including 17 PRL family members. Relative quantitative analysis between embryonic day 17 (E17) and E18 placentas showed no significant difference in the expression of PRL3B1 and most PRL family members except PRL7C1. These results suggest that PRL3B1 secretion from the placenta is suppressed at G18 (E18).

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来源期刊
Endocrine journal
Endocrine journal 医学-内分泌学与代谢
CiteScore
4.30
自引率
5.00%
发文量
224
审稿时长
1.5 months
期刊介绍: Endocrine Journal is an open access, peer-reviewed online journal with a long history. This journal publishes peer-reviewed research articles in multifaceted fields of basic, translational and clinical endocrinology. Endocrine Journal provides a chance to exchange your ideas, concepts and scientific observations in any area of recent endocrinology. Manuscripts may be submitted as Original Articles, Notes, Rapid Communications or Review Articles. We have a rapid reviewing and editorial decision system and pay a special attention to our quick, truly scientific and frequently-citable publication. Please go through the link for author guideline.
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