Vadde Ramu , Lukas S. Wijaya , Nataliia Beztsinna , Corjan Van de Griend , Bob van de Water , Sylvestre Bonnet , Sylvia E. Le Dévédec
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引用次数: 0
摘要
著名的 "光开关 "钌复合物 [Ru(bpy)2(dppz)](PF6)2 (1) 因其 DNA 体外结合特性而闻名已久。然而，这种化合物在活细胞中的细胞吸收率较低，阻碍了它在生物学上的应用。在此，我们报告了 1 作为细胞存活率探针在二维细胞单层和三维多细胞肿瘤球体（U87、HepG2、A549）中的生物成像应用。与常规使用的细胞活力探针碘化丙啶相比，1 能增强死细胞的染色效果，尤其是在肿瘤球体内。与已知会致癌的碘化丙啶相比，1 具有较高的光稳定性、较长的斯托克斯位移和较低的细胞毒性。最后，研究还发现 1 能取代死细胞中传统的 DNA 结合染料 Hoechst，这使它成为一种很有前途的染料，可用于对细胞培养物（包括多细胞肿瘤球体）中的死细胞进行时间依赖性成像。
Cell viability imaging in tumor spheroids via DNA binding of a ruthenium(ii) light-switch complex†
The famous ‘‘light-switch’’ ruthenium complex [Ru(bpy)2(dppz)](PF6)2 (1) has been long known for its DNA binding properties in vitro. However, the biological utility of this compound has been hampered by its poor cellular uptake in living cells. Here we report a bioimaging application of 1 as cell viability probe in both 2D cells monolayer and 3D multi-cellular tumor spheroids of various human cancer cell lines (U87, HepG2, A549). When compared to propidium iodide, a routinely used cell viability probe, 1 was found to enhance the staining of dead cells in particular in tumor spheroids. 1 has high photostability, longer Stokes shift, and displays lower cytotoxicity compared to propidium iodide, which is a known carcinogenic. Finally, 1 was also found to displace the classical DNA binding dye Hoechst in dead cells, which makes it a promising dye for time-dependent imaging of dead cells in cell cultures, including multi-cellular tumor spheroids.
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