高血压脑出血患者血浆外泌体衍生环状 RNA 的特征和功能

IF 2.3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Kejie Chen, Xiaoyuan Cheng, Shanshan Yuan, Yang Sun, Junli Hao, Quandan Tan, Yapeng Lin, Shuping Li, Jie Yang
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引用次数: 0

摘要

脑出血(ICH)是导致死亡和残疾的主要原因之一,而高血压性 ICH(HICH)是最常见的 ICH 类型。目前,HICH 患者治疗后的预后仍然不佳,因此对 HICH 的早期预后预测非常重要。然而,目前针对 HICH 患者的有效临床治疗方法和生物标志物非常有限。尽管循环RNA在疾病中已被广泛研究,但血浆外泌体循环RNA在HICH中的作用仍然未知。本研究利用 circRNA 微阵列和生物信息学分析,研究了 6 例 HICH 患者血浆外泌体 circRNA 的特征和功能。结果表明,HICH 患者和对照组之间有 499 个不同表达的外泌体 circRNA。根据GO注释和KEGG通路分析,差异表达的外泌体circRNA所调控的靶点通过神经/神经元生长、神经炎症和内皮稳态与HICH的发病密切相关。不同表达的外泌体circRNAs主要与四种RNA结合蛋白(EIF4A3、FMRP、AGO2和HUR)结合。此外,在差异表达的外泌体循环RNA中,hsa_circ_00054843、hsa_circ_0010493和hsa_circ_00090516与受试者的出血量和格拉斯哥昏迷量表评分显著相关。我们的研究结果首次揭示了血浆外泌体 circRNAs 与 HICH 的进展密切相关,可作为 HICH 的有效生物标志物。这为进一步研究确定最佳生物标志物和阐明外泌体 circRNAs 在 HICH 中的作用机制奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Signature and function of plasma exosome-derived circular RNAs in patients with hypertensive intracerebral hemorrhage.

Signature and function of plasma exosome-derived circular RNAs in patients with hypertensive intracerebral hemorrhage.

Intracerebral hemorrhage (ICH) is one of the major causes of death and disability, and hypertensive ICH (HICH) is the most common type of ICH. Currently, the outcomes of HICH patients remain poor after treatment, and early prognosis prediction of HICH is important. However, there are limited effective clinical treatments and biomarkers for HICH patients. Although circRNA has been widely studied in diseases, the role of plasma exosomal circRNAs in HICH remains unknown. The present study was conducted to investigate the characteristics and function of plasma exosomal circRNAs in six HICH patients using circRNA microarray and bioinformatics analysis. The results showed that there were 499 differentially expressed exosomal circRNAs between the HICH patients and control subjects. According to GO annotation and KEGG pathway analyses, the targets regulated by differentially expressed exosomal circRNAs were tightly related to the development of HICH via nerve/neuronal growth, neuroinflammation and endothelial homeostasis. And the differentially expressed exosomal circRNAs could mainly bind to four RNA-binding proteins (EIF4A3, FMRP, AGO2 and HUR). Moreover, of differentially expressed exosomal circRNAs, hsa_circ_00054843, hsa_circ_0010493 and hsa_circ_00090516 were significantly associated with bleeding volume and Glasgow Coma Scale score of the subjects. Our findings firstly revealed that the plasma exosomal circRNAs are significantly involved in the progression of HICH, and could be potent biomarkers for HICH. This provides the basis for further research to pinpoint the best biomarkers and illustrate the mechanism of exosomal circRNAs in HICH.

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来源期刊
Molecular Genetics and Genomics
Molecular Genetics and Genomics 生物-生化与分子生物学
CiteScore
5.10
自引率
3.20%
发文量
134
审稿时长
1 months
期刊介绍: Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic. MGG publishes research on all organisms that is of broad interest to those working in the fields of genetics, genomics, biology, medicine and biotechnology. The journal investigates a broad range of topics, including these from recent issues: mechanisms for extending longevity in a variety of organisms; screening of yeast metal homeostasis genes involved in mitochondrial functions; molecular mapping of cultivar-specific avirulence genes in the rice blast fungus and more.
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