Ya Bin Zhou, Ali Rezaei-Matehkolaei, Martin Meijer, Bart Kraak, Bert Gerrits van den Ende, Ferry Hagen, Sara Afzalzadeh, Neda Kiasat, Ameneh Takesh, Akbar Hoseinnejad, Jos Houbraken
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引用次数: 0
摘要
一名曾被诊断患有肺结核和肺空洞的 50 岁男子出现了发烧、气短和咳嗽等症状。肺部 CT 扫描显示上肺有多个空洞、合并症和树状脓疱。通过对支气管肺泡灌洗液的直接检查进行进一步调查,发现了具有二歧急性分支的隔膜菌丝。经过分离和形态学分析,确定该真菌属于黑曲霉。患者被诊断为可能的侵袭性肺曲霉菌病,并成功接受了为期三个月的伏立康唑口服治疗。根据部分β-微管蛋白、钙调蛋白和RNA聚合酶第二大亚基序列进行的系统发育分析表明,该分离株代表了与巴西曲霉有关的推定新种,在此命名为Hubkae曲霉。抗真菌药敏试验表明,该分离株对伊曲康唑有抗药性,但对伏立康唑敏感。对 A. hubkae 进行的表型和基因鉴定以及相关的病例报告,将为今后诊断由该菌种引起的感染提供宝贵的资料。它还将有助于在类似的临床情况下制定更精确、更有效的患者管理策略。
Aspergillus hubkae, a Novel Species Isolated from a Patient with Probable Invasive Pulmonary Aspergillosis.
A 50-year-old man, previously diagnosed with pulmonary tuberculosis and lung cavities, presented with symptoms including fever, shortness of breath, and cough. A pulmonary CT scan revealed multiple cavities, consolidation and tree-in-bud in the upper lungs. Further investigation through direct examination of bronchoalveolar lavage fluid showed septate hyphae with dichotomous acute branching. Subsequent isolation and morphological analysis identified the fungus as belonging to Aspergillus section Nigri. The patient was diagnosed with probable invasive pulmonary aspergillosis and successfully treated with a three-month oral voriconazole therapy. Phylogenetic analysis based on partial β-tubulin, calmodulin and RNA polymerase second largest subunit sequences revealed that the isolate represents a putative new species related to Aspergillus brasiliensis, and is named Aspergillus hubkae here. Antifungal susceptibility testing demonstrated that the isolate is resistant to itraconazole but susceptible to voriconazole. This phenotypic and genetic characterization of A. hubkae, along with the associated case report, will serve as a valuable resource for future diagnoses of infections caused by this species. It will also contribute to more precise and effective patient management strategies in similar clinical scenarios.
期刊介绍:
Mycopathologia is an official journal of the International Union of Microbiological Societies (IUMS). Mycopathologia was founded in 1938 with the mission to ‘diffuse the understanding of fungal diseases in man and animals among mycologists’. Many of the milestones discoveries in the field of medical mycology have been communicated through the pages of this journal. Mycopathologia covers a diverse, interdisciplinary range of topics that is unique in breadth and depth. The journal publishes peer-reviewed, original articles highlighting important developments concerning medically important fungi and fungal diseases. The journal highlights important developments in fungal systematics and taxonomy, laboratory diagnosis of fungal infections, antifungal drugs, clinical presentation and treatment, and epidemiology of fungal diseases globally. Timely opinion articles, mini-reviews, and other communications are usually invited at the discretion of the editorial board. Unique case reports highlighting unprecedented progress in the diagnosis and treatment of fungal infections, are published in every issue of the journal. MycopathologiaIMAGE is another regular feature for a brief clinical report of potential interest to a mixed audience of physicians and laboratory scientists. MycopathologiaGENOME is designed for the rapid publication of new genomes of human and animal pathogenic fungi using a checklist-based, standardized format.