血管活性肠肽在人类间充质干细胞中发挥骨诱导作用。

IF 5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
BioFactors Pub Date : 2024-05-11 DOI:10.1002/biof.2062
David Castro-Vázquez, Paula Arribas-Castaño, Iván García-López, Irene Gutiérrez-Cañas, Selene Pérez-García, Amalia Lamana, Raúl Villanueva-Romero, Alicia Cabrera-Martín, Karolina Tecza, Carmen Martínez, Yasmina Juarranz, Rosa P Gomariz, Mar Carrión
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引用次数: 0

摘要

据报道,存在于骨组织中由神经纤维和骨细胞产生的几种神经肽在调节成骨细胞和破骨细胞功能的微调以维持骨平衡方面发挥作用。本研究旨在阐明神经肽血管活性肠肽(VIP)对人间充质干细胞(MSCs)向成骨细胞分化过程及其合成代谢功能的影响。我们描述了VIP及其受体在间充质干细胞分化成成骨细胞过程中的mRNA和蛋白质表达谱,表明这些细胞中存在自分泌信号通路。我们的研究结果表明,VIP 能增强成骨分化中间叶干细胞中早期成骨细胞标志物的表达,并有利于骨基质的形成和适当的细胞骨架重组。最后,我们的数据表明,VIP 可通过下调核因子κB 配体受体激活剂/破骨细胞蛋白的比例,直接调节成骨细胞与破骨细胞之间的信号传导。这些结果凸显了 VIP 作为骨诱导分化因子的潜力,它正在成为维持人体骨平衡的关键分子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Vasoactive intestinal peptide exerts an osteoinductive effect in human mesenchymal stem cells.

Vasoactive intestinal peptide exerts an osteoinductive effect in human mesenchymal stem cells.

Several neuropeptides present in bone tissues, produced by nerve fibers and bone cells, have been reported to play a role in regulating the fine-tuning of osteoblast and osteoclast functions to maintain bone homeostasis. This study aims to characterize the influence of the neuropeptide vasoactive intestinal peptide (VIP) on the differentiation process of human mesenchymal stem cells (MSCs) into osteoblasts and on their anabolic function. We describe the mRNA and protein expression profile of VIP and its receptors in MSCs as they differentiate into osteoblasts, suggesting the presence of an autocrine signaling pathway in these cells. Our findings reveal that VIP enhances the expression of early osteoblast markers in MSCs under osteogenic differentiation and favors both bone matrix formation and proper cytoskeletal reorganization. Finally, our data suggest that VIP could be exerting a direct modulatory role on the osteoblast to osteoclast signaling by downregulating the receptor activator of nuclear factor-κB ligand/osteoprotegerin ratio. These results highlight the potential of VIP as an osteoinductive differentiation factor, emerging as a key molecule in the maintenance of human bone homeostasis.

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来源期刊
BioFactors
BioFactors 生物-内分泌学与代谢
CiteScore
11.50
自引率
3.30%
发文量
96
审稿时长
6-12 weeks
期刊介绍: BioFactors, a journal of the International Union of Biochemistry and Molecular Biology, is devoted to the rapid publication of highly significant original research articles and reviews in experimental biology in health and disease. The word “biofactors” refers to the many compounds that regulate biological functions. Biological factors comprise many molecules produced or modified by living organisms, and present in many essential systems like the blood, the nervous or immunological systems. A non-exhaustive list of biological factors includes neurotransmitters, cytokines, chemokines, hormones, coagulation factors, transcription factors, signaling molecules, receptor ligands and many more. In the group of biofactors we can accommodate several classical molecules not synthetized in the body such as vitamins, micronutrients or essential trace elements. In keeping with this unified view of biochemistry, BioFactors publishes research dealing with the identification of new substances and the elucidation of their functions at the biophysical, biochemical, cellular and human level as well as studies revealing novel functions of already known biofactors. The journal encourages the submission of studies that use biochemistry, biophysics, cell and molecular biology and/or cell signaling approaches.
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