Chuntan Chen , Yu Wang , Qunwei Dai , Weiqi Du , Xinshuang Deng , Yulian Zhao , Qian Duan , Hepei Liu
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The results showed that the symbiotic bacteria significantly enhanced the fixation of 15 % CO<sub>2</sub> by <em>Chlorella</em>, with <em>C. vulgaris</em> HL02 + <em>Aeromonas</em> GS-HL01 having the highest carbon fixation efficiency, which was 22.31 % higher than that of the sterile control, followed by <em>C. vulgaris</em> HL02 + <em>Bacillus</em> GS-F03, and <em>C. vulgaris</em> HL02 + <em>Microbacterium</em> GS-H02 (which was also 16.36 % higher than the sterile control). The composition of harvested <em>Chlorella</em> was not altered significantly following co-culturing with bacteria. Scanning electron microscopy and Excitation–emission matrix spectra analyses showed that at high concentrations of CO<sub>2</sub>, Microalgae and bacteria promote mutual growth and CO<sub>2</sub> fixation mainly through extracellular secretion of organic matter. Our results provided a new strategy to improve the efficiency of microalgae in fixing high concentration of CO<sub>2</sub>.</p></div>","PeriodicalId":350,"journal":{"name":"Journal of CO2 Utilization","volume":null,"pages":null},"PeriodicalIF":7.2000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2212982024001380/pdfft?md5=0b99e3c3ae2e14cd2c8c1354d7d45c43&pid=1-s2.0-S2212982024001380-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Fixation of high concentration CO2 using Chlorella – Bacteria symbiosis system\",\"authors\":\"Chuntan Chen , Yu Wang , Qunwei Dai , Weiqi Du , Xinshuang Deng , Yulian Zhao , Qian Duan , Hepei Liu\",\"doi\":\"10.1016/j.jcou.2024.102803\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The mutualistic interaction between microalgae and bacteria provides a new strategy for improving carbon fixation by microalgae. In this study, <em>Chlorella</em> was co-cultured with natural flora at 15 % (v/v) CO<sub>2</sub> concentration for 120 d. Three <em>Chlorella</em> symbiotic bacteria strains were identified and were co-cultured with <em>Chlorella vulgaris</em> HL02 (<em>C. vulgaris</em> HL02) to fix high concentrations of CO<sub>2</sub>. The results showed that the symbiotic bacteria significantly enhanced the fixation of 15 % CO<sub>2</sub> by <em>Chlorella</em>, with <em>C. vulgaris</em> HL02 + <em>Aeromonas</em> GS-HL01 having the highest carbon fixation efficiency, which was 22.31 % higher than that of the sterile control, followed by <em>C. vulgaris</em> HL02 + <em>Bacillus</em> GS-F03, and <em>C. vulgaris</em> HL02 + <em>Microbacterium</em> GS-H02 (which was also 16.36 % higher than the sterile control). The composition of harvested <em>Chlorella</em> was not altered significantly following co-culturing with bacteria. 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引用次数: 0
摘要
微藻类与细菌之间的相互影响为改善微藻类的碳固定提供了一种新策略。在这项研究中,小球藻与天然菌群在二氧化碳浓度为 15% (v/v) 的条件下共培养 120 天。鉴定了三种小球藻共生菌株,并将其与绿球藻 HL02(C. vulgaris HL02)共培养,以固定高浓度的二氧化碳。结果表明,共生菌显著提高了小球藻固定 15% CO2 的能力,其中 C. vulgaris HL02 + 气单胞菌 GS-HL01 的固碳效率最高,比无菌对照高 22.31%,其次是 C. vulgaris HL02 + 芽孢杆菌 GS-F03 和 C. vulgaris HL02 + 微杆菌 GS-H02(也比无菌对照高 16.36%)。与细菌共培养后,收获的小球藻的成分没有明显变化。扫描电子显微镜和激发-发射矩阵光谱分析表明,在高浓度二氧化碳条件下,微藻和细菌主要通过胞外分泌有机物促进相互生长和二氧化碳固定。我们的研究结果为提高微藻固定高浓度二氧化碳的效率提供了一种新策略。
Fixation of high concentration CO2 using Chlorella – Bacteria symbiosis system
The mutualistic interaction between microalgae and bacteria provides a new strategy for improving carbon fixation by microalgae. In this study, Chlorella was co-cultured with natural flora at 15 % (v/v) CO2 concentration for 120 d. Three Chlorella symbiotic bacteria strains were identified and were co-cultured with Chlorella vulgaris HL02 (C. vulgaris HL02) to fix high concentrations of CO2. The results showed that the symbiotic bacteria significantly enhanced the fixation of 15 % CO2 by Chlorella, with C. vulgaris HL02 + Aeromonas GS-HL01 having the highest carbon fixation efficiency, which was 22.31 % higher than that of the sterile control, followed by C. vulgaris HL02 + Bacillus GS-F03, and C. vulgaris HL02 + Microbacterium GS-H02 (which was also 16.36 % higher than the sterile control). The composition of harvested Chlorella was not altered significantly following co-culturing with bacteria. Scanning electron microscopy and Excitation–emission matrix spectra analyses showed that at high concentrations of CO2, Microalgae and bacteria promote mutual growth and CO2 fixation mainly through extracellular secretion of organic matter. Our results provided a new strategy to improve the efficiency of microalgae in fixing high concentration of CO2.
期刊介绍:
The Journal of CO2 Utilization offers a single, multi-disciplinary, scholarly platform for the exchange of novel research in the field of CO2 re-use for scientists and engineers in chemicals, fuels and materials.
The emphasis is on the dissemination of leading-edge research from basic science to the development of new processes, technologies and applications.
The Journal of CO2 Utilization publishes original peer-reviewed research papers, reviews, and short communications, including experimental and theoretical work, and analytical models and simulations.