Xiao Hong Li, Yu Mei Liu, Hui Shan, Jin Feng Tan, Jian Zhou, Yuan Jin Song, Si Qi Li, Chen Liu, Dong Qun Xu, Li Yu, Wan Wei Li
{"title":"水杨甙通过调节小鼠体内的SIRT1-PGC-1α改善PM 2.5诱发的肺损伤","authors":"Xiao Hong Li, Yu Mei Liu, Hui Shan, Jin Feng Tan, Jian Zhou, Yuan Jin Song, Si Qi Li, Chen Liu, Dong Qun Xu, Li Yu, Wan Wei Li","doi":"10.3967/bes2024.041","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to clarify the intervention effect of salidroside (SAL) on lung injury caused by PM <sub>2.5</sub> in mice and illuminate the function of SIRT1-PGC-1ɑ axis.</p><p><strong>Methods: </strong>Specific pathogen-free (SPF) grade male C57BL/6 mice were randomly assigned to the following groups: control group, SAL group, PM <sub>2.5</sub> group, SAL+PM <sub>2.5</sub> group. On the first day, SAL was given by gavage, and on the second day, PM <sub>2.5</sub> suspension was given by intratracheal instillation. The whole experiment consist of a total of 10 cycles, lasting 20 days. At the end of treatment, blood samples and lung tissues were collected and analyzed. Observation of pathological changes in lung tissue using inverted microscopy and transmission electron microscopy. The expression of inflammatory, antioxidants, apoptosis, and SIRT1-PGC-1ɑ proteins were detected by Western blotting.</p><p><strong>Results: </strong>Exposure to PM <sub>2.5</sub> leads to obvious morphological and pathologica changes in the lung of mice. PM <sub>2.5</sub> caused a decline in levels of antioxidant-related enzymes and protein expressions of HO-1, Nrf2, SOD2, SIRT1 and PGC-1ɑ, and an increase in the protein expressions of IL-6, IL-1β, Bax, caspase-9 and cleaved caspase-3. However, SAL reversed the aforementioned changes caused by PM <sub>2.5</sub> by activating the SIRT1-PGC-1α pathway.</p><p><strong>Conclusion: </strong>SAL can activate SIRT1-PGC-1ɑ to ameliorate PM <sub>2.5</sub>-induced lung injury.</p>","PeriodicalId":93903,"journal":{"name":"Biomedical and environmental sciences : BES","volume":"37 4","pages":"367-376"},"PeriodicalIF":0.0000,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Salidroside Ameliorates Lung Injury Induced by PM <sub>2.5</sub>by Regulating SIRT1-PGC-1α in Mice.\",\"authors\":\"Xiao Hong Li, Yu Mei Liu, Hui Shan, Jin Feng Tan, Jian Zhou, Yuan Jin Song, Si Qi Li, Chen Liu, Dong Qun Xu, Li Yu, Wan Wei Li\",\"doi\":\"10.3967/bes2024.041\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study aimed to clarify the intervention effect of salidroside (SAL) on lung injury caused by PM <sub>2.5</sub> in mice and illuminate the function of SIRT1-PGC-1ɑ axis.</p><p><strong>Methods: </strong>Specific pathogen-free (SPF) grade male C57BL/6 mice were randomly assigned to the following groups: control group, SAL group, PM <sub>2.5</sub> group, SAL+PM <sub>2.5</sub> group. On the first day, SAL was given by gavage, and on the second day, PM <sub>2.5</sub> suspension was given by intratracheal instillation. The whole experiment consist of a total of 10 cycles, lasting 20 days. At the end of treatment, blood samples and lung tissues were collected and analyzed. Observation of pathological changes in lung tissue using inverted microscopy and transmission electron microscopy. The expression of inflammatory, antioxidants, apoptosis, and SIRT1-PGC-1ɑ proteins were detected by Western blotting.</p><p><strong>Results: </strong>Exposure to PM <sub>2.5</sub> leads to obvious morphological and pathologica changes in the lung of mice. PM <sub>2.5</sub> caused a decline in levels of antioxidant-related enzymes and protein expressions of HO-1, Nrf2, SOD2, SIRT1 and PGC-1ɑ, and an increase in the protein expressions of IL-6, IL-1β, Bax, caspase-9 and cleaved caspase-3. However, SAL reversed the aforementioned changes caused by PM <sub>2.5</sub> by activating the SIRT1-PGC-1α pathway.</p><p><strong>Conclusion: </strong>SAL can activate SIRT1-PGC-1ɑ to ameliorate PM <sub>2.5</sub>-induced lung injury.</p>\",\"PeriodicalId\":93903,\"journal\":{\"name\":\"Biomedical and environmental sciences : BES\",\"volume\":\"37 4\",\"pages\":\"367-376\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical and environmental sciences : BES\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3967/bes2024.041\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical and environmental sciences : BES","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3967/bes2024.041","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Salidroside Ameliorates Lung Injury Induced by PM 2.5by Regulating SIRT1-PGC-1α in Mice.
Objective: This study aimed to clarify the intervention effect of salidroside (SAL) on lung injury caused by PM 2.5 in mice and illuminate the function of SIRT1-PGC-1ɑ axis.
Methods: Specific pathogen-free (SPF) grade male C57BL/6 mice were randomly assigned to the following groups: control group, SAL group, PM 2.5 group, SAL+PM 2.5 group. On the first day, SAL was given by gavage, and on the second day, PM 2.5 suspension was given by intratracheal instillation. The whole experiment consist of a total of 10 cycles, lasting 20 days. At the end of treatment, blood samples and lung tissues were collected and analyzed. Observation of pathological changes in lung tissue using inverted microscopy and transmission electron microscopy. The expression of inflammatory, antioxidants, apoptosis, and SIRT1-PGC-1ɑ proteins were detected by Western blotting.
Results: Exposure to PM 2.5 leads to obvious morphological and pathologica changes in the lung of mice. PM 2.5 caused a decline in levels of antioxidant-related enzymes and protein expressions of HO-1, Nrf2, SOD2, SIRT1 and PGC-1ɑ, and an increase in the protein expressions of IL-6, IL-1β, Bax, caspase-9 and cleaved caspase-3. However, SAL reversed the aforementioned changes caused by PM 2.5 by activating the SIRT1-PGC-1α pathway.
Conclusion: SAL can activate SIRT1-PGC-1ɑ to ameliorate PM 2.5-induced lung injury.
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