麦芽糊精纳米颗粒中的弓形虫抗原对人类 T 细胞的激活作用

IF 3.3 2区 生物学 Q1 BIOLOGY
Life Science Alliance Pub Date : 2024-05-09 Print Date: 2024-07-01 DOI:10.26508/lsa.202302486
Laura García-López, Alejandro Zamora-Vélez, Mónica Vargas-Montes, Juan Camilo Sanchez-Arcila, François Fasquelle, Didier Betbeder, Jorge Enrique Gómez-Marín
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引用次数: 0

摘要

弓形虫病是全球最普遍的寄生虫动物传染病,可引起眼部和神经系统疾病。目前还没有疫苗被批准用于人类。我们评估了不同感染状态(未感染、慢性无症状或眼部弓形虫病)的个体的外周血单核细胞(PBMC)对麦芽糊精纳米颗粒(NP/TE)中弓形虫总抗原的新型构建物的反应。我们使用 ELISA 分析了 NP/TE 体外刺激后 IFN-γ 的浓度,并使用流式细胞术分析了 CD4+ 和 CD8+ 细胞群的免疫分型。此外,还利用源自 GRA6 的多肽通过 ELISA 对弓形虫感染者进行了血清分型。低剂量的 NP/TE 刺激(0.9 μg NP/0.3 μg TE)可使先前暴露的人类 PBMC 产生 IFN-γ 特异性,但感染血清型无显著差异。在使用 NP/TE 的眼弓形虫病患者中,CD4+效应记忆细胞亚群中的 IFN-γ 表达增加,而单独使用 TE 则没有增加。这是第一项显示T细胞亚群如何对人类弓形虫候选疫苗的体外刺激做出反应的研究,为未来的临床试验提供了重要的启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Human T-cell activation with Toxoplasma gondii antigens loaded in maltodextrin nanoparticles.

Toxoplasmosis is the most prevalent parasitic zoonosis worldwide, causing ocular and neurological diseases. No vaccine has been approved for human use. We evaluated the response of peripheral blood mononuclear cells (PBMCs) to a novel construct of Toxoplasma gondii total antigen in maltodextrin nanoparticles (NP/TE) in individuals with varying infectious statuses (uninfected, chronic asymptomatic, or ocular toxoplasmosis). We analyzed the concentration of IFN-γ after NP/TE ex vivo stimulation using ELISA and the immunophenotypes of CD4+ and CD8+ cell populations using flow cytometry. In addition, serotyping of individuals with toxoplasmosis was performed by ELISA using GRA6-derived polypeptides. Low doses of NP/TE stimulation (0.9 μg NP/0.3 μg TE) achieved IFN-γ-specific production in previously exposed human PBMCs without significant differences in the infecting serotype. Increased IFN-γ expression in CD4+ effector memory cell subsets was found in patients with ocular toxoplasmosis with NP/TE but not with TE alone. This is the first study to show how T-cell subsets respond to ex vivo stimulation with a vaccine candidate for human toxoplasmosis, providing crucial insights for future clinical trials.

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来源期刊
Life Science Alliance
Life Science Alliance Agricultural and Biological Sciences-Plant Science
CiteScore
5.80
自引率
2.30%
发文量
241
审稿时长
10 weeks
期刊介绍: Life Science Alliance is a global, open-access, editorially independent, and peer-reviewed journal launched by an alliance of EMBO Press, Rockefeller University Press, and Cold Spring Harbor Laboratory Press. Life Science Alliance is committed to rapid, fair, and transparent publication of valuable research from across all areas in the life sciences.
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