超高效液相色谱-质谱/质谱法同时定量测定人血浆中的万古霉素、利奈唑胺和伏立康唑：在治疗药物监测中的应用

IF 1.5 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of chromatographic science Pub Date : 2024-08-20 DOI:10.1093/chromsci/bmae017

Yao Liu, Yanan Li, Wenli Chen, Dandan Ming, Wenji Luo, Jiabi Liang

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引用次数: 0

摘要

目的：个体差异对万古霉素、利奈唑胺和伏立康唑治疗严重感染提出了挑战。本研究旨在通过超高效液相色谱-串联质谱法（UHPLC-MS/MS）建立一种简单经济的方法，同时测定人体血浆中的三种抗生素，为感染患者的治疗药物监测（TDM）提供参考：血浆样品经乙腈沉淀后，在 5 分钟内用垫片包装的 GIST C18 色谱柱进行梯度洗脱检测和分离。结果：万古霉素在血浆中的线性范围为 1.0～10.0μg/L，相对标准偏差（RSD）为 1.0～10.0μg/L：万古霉素、利奈唑胺和伏立康唑的线性范围分别为1.00-100.00、0.10-15.00和0.10-20.00 μg-mL-1，线性关系良好（R2 > 0.99）。该方法的准确度、精密度、基质效应、提取回收率和稳定性均符合中国食品药品监督管理局（CFDA）的标准：结论：建立了同时测定人血浆中万古霉素、利奈唑胺和伏立康唑的超高效液相色谱-质谱/质谱方法，并将其成功应用于个体化治疗的常规TDM中。

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Simultaneous Quantification of Vancomycin, Linezolid and Voriconazole in Human Plasma by UHPLC-MS/MS: Application in Therapeutic Drug Monitoring.

Objective: Individual differences challenge the treatment of vancomycin, linezolid and voriconazole in severe infections. This study aimed to build a simple and economical method for simultaneous determination of the three antibiotics in human plasma by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and provided a reference for therapeutic drug monitoring (TDM) of infected patients.

Methods: The plasma samples were precipitated by acetonitrile and detected and separated on a shim-pack GIST C18 column following the gradient elution within 5 min. Mass quantification was performed on multiple reaction monitoring mode under positive electrospray ionization.

Results: The linear ranges of vancomycin, linezolid and voriconazole were 1.00-100.00, 0.10-15.00 and 0.10-20.00 μg·mL-1, respectively, with good linearity (R2 > 0.99). The accuracy and precision, matrix effect, extraction recovery and stability were validated, and the results all meet the acceptance criteria of China Food and Drug Administration (CFDA) guidelines.

Conclusion: The UHPLC-MS/MS method was established and validated for the simultaneous determination of vancomycin, linezolid and voriconazole in human plasma and successfully applied to routine TDM for individualized treatment.

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.