Viktoria Ehret MSc, Usevalad Ustsinau MSc, Joachim Friske Mag, Thomas Scherer MD, Clemens Fürnsinn PhD, Thomas H. Helbich MD, Cécile Philippe PhD, Martin Krššák PhD
{"title":"利用氘代谢成像评估高脂啮齿动物的肝糖和棕榈酸代谢情况","authors":"Viktoria Ehret MSc, Usevalad Ustsinau MSc, Joachim Friske Mag, Thomas Scherer MD, Clemens Fürnsinn PhD, Thomas H. Helbich MD, Cécile Philippe PhD, Martin Krššák PhD","doi":"10.1002/jmri.29437","DOIUrl":null,"url":null,"abstract":"<div>\n \n <section>\n \n <h3> Background</h3>\n \n <p>One of the main features of several metabolic disorders is dysregulation of hepatic glucose and lipid metabolism. Deuterium metabolic imaging (DMI) allows for assessing the uptake and breakdown of <sup>2</sup>H-labeled substrates, giving specific insight into nutrient processing in healthy and diseased organs. Thus, DMI could be a useful approach for analyzing the differences in liver metabolism of healthy and diseased subjects to gain a deeper understanding of the alterations related to metabolic disorders.</p>\n </section>\n \n <section>\n \n <h3> Purpose</h3>\n \n <p>Evaluating the feasibility of DMI as a tool for the assessment of metabolic differences in rodents with healthy and fatty livers (FLs).</p>\n </section>\n \n <section>\n \n <h3> Study Type</h3>\n \n <p>Animal Model.</p>\n </section>\n \n <section>\n \n <h3> Population</h3>\n \n <p>18 male Sprague Dawley rats on standard (SD, <i>n</i> = 9, healthy) and high-fat diet (HFD, <i>n</i> = 9, FL disease).</p>\n </section>\n \n <section>\n \n <h3> Field Strength/Sequence</h3>\n \n <p>Phase-encoded 1D pulse-acquire sequence and anatomy co-registered phase-encoded 3D pulse-acquire chemical shift imaging for <sup>2</sup>H at 9.4T.</p>\n </section>\n \n <section>\n \n <h3> Assessment</h3>\n \n <p>Localized and nonlocalized liver spectroscopy was applied at eight time points over 104 minutes post injection. The obtained spectra were preprocessed and quantified using jMRUI (v7.0) and the resulting amplitudes translated to absolute concentration (mM) according to the <sup>2</sup>H natural abundance water peak.</p>\n </section>\n \n <section>\n \n <h3> Statistical Tests</h3>\n \n <p>Two-way repeated measures ANOVA were employed to assess between-group differences, with statistical significance at <i>P</i> < 0.05.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>DMI measurements demonstrated no significant difference (<i>P</i> = 0.98) in the uptake of [6,6′-<sup>2</sup>H<sub>2</sub>]glucose between healthy and impaired animals (AUC<sub>SD</sub> = 1966.0 ± 151.5 mM - minutes vs. AUC<sub>HFD</sub> = 2027.0 ± 167.6 mM·minutes). In the diseased group, the intrahepatic uptake of palmitic acid d-31 was higher (AUC<sub>HFD</sub> = 57.4 ± 17.0 mM·minutes, AUC<sub>SD</sub> = 33.3 ± 10.5 mM·minutes), but without statistical significance owing to substantial in-group variation (<i>P</i> = 0.73).</p>\n </section>\n \n <section>\n \n <h3> Data Conclusion</h3>\n \n <p>DMI revealed higher concentrations of palmitic acid in rats with FL disease and no difference in hepatic glucose concentration between healthy and impaired animals. Thus, DMI appears to be a useful tool for evaluating metabolism in rodents with FL disease.</p>\n </section>\n \n <section>\n \n <h3> Level of Evidence</h3>\n \n <p>2</p>\n </section>\n \n <section>\n \n <h3> Technical Efficacy</h3>\n \n <p>Stage 3.</p>\n </section>\n </div>","PeriodicalId":16140,"journal":{"name":"Journal of Magnetic Resonance Imaging","volume":"61 2","pages":"958-967"},"PeriodicalIF":3.3000,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11706318/pdf/","citationCount":"0","resultStr":"{\"title\":\"Evaluation of Hepatic Glucose and Palmitic Acid Metabolism in Rodents on High-Fat Diet Using Deuterium Metabolic Imaging\",\"authors\":\"Viktoria Ehret MSc, Usevalad Ustsinau MSc, Joachim Friske Mag, Thomas Scherer MD, Clemens Fürnsinn PhD, Thomas H. Helbich MD, Cécile Philippe PhD, Martin Krššák PhD\",\"doi\":\"10.1002/jmri.29437\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>One of the main features of several metabolic disorders is dysregulation of hepatic glucose and lipid metabolism. Deuterium metabolic imaging (DMI) allows for assessing the uptake and breakdown of <sup>2</sup>H-labeled substrates, giving specific insight into nutrient processing in healthy and diseased organs. Thus, DMI could be a useful approach for analyzing the differences in liver metabolism of healthy and diseased subjects to gain a deeper understanding of the alterations related to metabolic disorders.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Purpose</h3>\\n \\n <p>Evaluating the feasibility of DMI as a tool for the assessment of metabolic differences in rodents with healthy and fatty livers (FLs).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Study Type</h3>\\n \\n <p>Animal Model.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Population</h3>\\n \\n <p>18 male Sprague Dawley rats on standard (SD, <i>n</i> = 9, healthy) and high-fat diet (HFD, <i>n</i> = 9, FL disease).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Field Strength/Sequence</h3>\\n \\n <p>Phase-encoded 1D pulse-acquire sequence and anatomy co-registered phase-encoded 3D pulse-acquire chemical shift imaging for <sup>2</sup>H at 9.4T.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Assessment</h3>\\n \\n <p>Localized and nonlocalized liver spectroscopy was applied at eight time points over 104 minutes post injection. The obtained spectra were preprocessed and quantified using jMRUI (v7.0) and the resulting amplitudes translated to absolute concentration (mM) according to the <sup>2</sup>H natural abundance water peak.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Statistical Tests</h3>\\n \\n <p>Two-way repeated measures ANOVA were employed to assess between-group differences, with statistical significance at <i>P</i> < 0.05.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>DMI measurements demonstrated no significant difference (<i>P</i> = 0.98) in the uptake of [6,6′-<sup>2</sup>H<sub>2</sub>]glucose between healthy and impaired animals (AUC<sub>SD</sub> = 1966.0 ± 151.5 mM - minutes vs. AUC<sub>HFD</sub> = 2027.0 ± 167.6 mM·minutes). In the diseased group, the intrahepatic uptake of palmitic acid d-31 was higher (AUC<sub>HFD</sub> = 57.4 ± 17.0 mM·minutes, AUC<sub>SD</sub> = 33.3 ± 10.5 mM·minutes), but without statistical significance owing to substantial in-group variation (<i>P</i> = 0.73).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Data Conclusion</h3>\\n \\n <p>DMI revealed higher concentrations of palmitic acid in rats with FL disease and no difference in hepatic glucose concentration between healthy and impaired animals. Thus, DMI appears to be a useful tool for evaluating metabolism in rodents with FL disease.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Level of Evidence</h3>\\n \\n <p>2</p>\\n </section>\\n \\n <section>\\n \\n <h3> Technical Efficacy</h3>\\n \\n <p>Stage 3.</p>\\n </section>\\n </div>\",\"PeriodicalId\":16140,\"journal\":{\"name\":\"Journal of Magnetic Resonance Imaging\",\"volume\":\"61 2\",\"pages\":\"958-967\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-05-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11706318/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Magnetic Resonance Imaging\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jmri.29437\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Magnetic Resonance Imaging","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jmri.29437","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING","Score":null,"Total":0}
Evaluation of Hepatic Glucose and Palmitic Acid Metabolism in Rodents on High-Fat Diet Using Deuterium Metabolic Imaging
Background
One of the main features of several metabolic disorders is dysregulation of hepatic glucose and lipid metabolism. Deuterium metabolic imaging (DMI) allows for assessing the uptake and breakdown of 2H-labeled substrates, giving specific insight into nutrient processing in healthy and diseased organs. Thus, DMI could be a useful approach for analyzing the differences in liver metabolism of healthy and diseased subjects to gain a deeper understanding of the alterations related to metabolic disorders.
Purpose
Evaluating the feasibility of DMI as a tool for the assessment of metabolic differences in rodents with healthy and fatty livers (FLs).
Study Type
Animal Model.
Population
18 male Sprague Dawley rats on standard (SD, n = 9, healthy) and high-fat diet (HFD, n = 9, FL disease).
Field Strength/Sequence
Phase-encoded 1D pulse-acquire sequence and anatomy co-registered phase-encoded 3D pulse-acquire chemical shift imaging for 2H at 9.4T.
Assessment
Localized and nonlocalized liver spectroscopy was applied at eight time points over 104 minutes post injection. The obtained spectra were preprocessed and quantified using jMRUI (v7.0) and the resulting amplitudes translated to absolute concentration (mM) according to the 2H natural abundance water peak.
Statistical Tests
Two-way repeated measures ANOVA were employed to assess between-group differences, with statistical significance at P < 0.05.
Results
DMI measurements demonstrated no significant difference (P = 0.98) in the uptake of [6,6′-2H2]glucose between healthy and impaired animals (AUCSD = 1966.0 ± 151.5 mM - minutes vs. AUCHFD = 2027.0 ± 167.6 mM·minutes). In the diseased group, the intrahepatic uptake of palmitic acid d-31 was higher (AUCHFD = 57.4 ± 17.0 mM·minutes, AUCSD = 33.3 ± 10.5 mM·minutes), but without statistical significance owing to substantial in-group variation (P = 0.73).
Data Conclusion
DMI revealed higher concentrations of palmitic acid in rats with FL disease and no difference in hepatic glucose concentration between healthy and impaired animals. Thus, DMI appears to be a useful tool for evaluating metabolism in rodents with FL disease.
期刊介绍：
The Journal of Magnetic Resonance Imaging (JMRI) is an international journal devoted to the timely publication of basic and clinical research, educational and review articles, and other information related to the diagnostic applications of magnetic resonance.
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