使用埃及凤凰木果核提取物进行抗菌、抗生物膜和抗病毒研究。

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Hanaa H Gomaa, Dalia Y Amin, Alaaeldin R Ahmed, Nader A Ismail, Khaled A El Dougdoug, Basma T Abd-Elhalim
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引用次数: 0

摘要

众所周知,Phoenix dactylifera L. 及其废弃物富含对人体健康有益的营养物质。本研究旨在体外评估凤凰木果核提取物(PDPE)的抗菌、抗生物膜和抗病毒特性。气相色谱-质谱(GC-MS)分析表明其中含有苯酚、2,5-双(1,1-二甲基乙基)、十四酸、八甘醇单十二烷基醚、á-D-吡喃葡萄糖苷酸和七甘醇单十二烷基醚。PDPE 对病原微生物有影响,抑制区直径(IZD)从 10.0 毫米到 35.0 毫米不等。金黄色葡萄球菌 ATCC 5638 的抑菌区直径最大,而伤寒沙门氏菌 DSM 17058 和宋内志贺氏菌 DSM 5570 的抑菌区直径最小。只有在孢子失效或分生孢子形成时才能观察到抗真菌效果。PDPE 对细菌的抗菌谱为 100%,MIC 值在 250 到 1000 µg/ml 之间。金黄色葡萄球菌的 MIC 值仅为 500 µg/ml。MBC 值介于 500 至 1000 克/毫升之间,对蜡样芽孢杆菌、粪大肠杆菌、伤寒杆菌和宋内氏杆菌的 MBC 值为 500 克/毫升。500 微克/毫升时的活性为 66.7%,浓度为 125-250 克/毫升时没有抗菌效果。PDPE 对金黄色葡萄球菌、蜡样芽孢杆菌(94.12%)和大肠杆菌(74.46%)的生物膜抑制率最高(98.59%)。在 50%(CC50)的病毒活性下,发现无毒的 PDPE 最高剂量为 123.0 µg/ml。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Antimicrobial, antibiofilm, and antiviral investigations using egyptian phoenix dactylifera L. pits extract.

Antimicrobial, antibiofilm, and antiviral investigations using egyptian phoenix dactylifera L. pits extract.

Phoenix dactylifera L. and its wastes are known to be high in nutrients that are beneficial to human health. The study aimed to evaluate the antimicrobial, antibiofilm, and antiviral properties of Phoenix dactylifera L. pits extract (PDPE) in vitro. Gas chromatography-mass spectrometry (GC-MS) analysis indicated phenol, 2,5-bis(1,1-dimethyl ethyl), tetradecanoic acid, octaethylene glycol monododecyl ether, á-D-glucopyranosiduronic acid, and heptaethylene glycol monododecyl ether existence. The PDPE influenced pathogenic microorganisms, with inhibition zone diameters (IZDs) ranging from 10.0 to 35.0 mm. Staphylococcus aureus ATCC 5638 had the highest IZD, while Salmonella typhi DSM 17058 and Shigella sonnei DSM 5570 had the lowest. The antifungal effect observed only in spore failure or conidia formation. PDPE showed a 100% antibacterial spectrum against bacteria, with MIC values between 250 and 1000 µg/ml. MIC was only indicated with S. aureus of 500 µg/ml. MBC values ranged from 500 to 1000 g/ml, with MBC values of 500 g/ml for B. cereus, E. faecalis, S. typhi, and S. sonnei. The activity was 66.7% at 500 µg/ml, further concentrations of 125-250 g/ml had no antibacterial effect. PDPE biofilm inhibition % had the highest percentage of inhibition (98.59%) with S. aureus, B. cereus (94.12%), and E. coli (74.46%). With 50% (CC50) viral activity, the highest non-toxic PDPE dose was found to be at 123.0 µg/ml.

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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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