组织学和单核转录组分析揭示了玉米叶状细纹细胞的特殊功能和叶角的关键调控因子

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Molecular Plant Pub Date : 2024-06-03 Epub Date: 2024-05-07 DOI:10.1016/j.molp.2024.05.001
Qibin Wang, Qiuyue Guo, Qingbiao Shi, Hengjia Yang, Meiling Liu, Yani Niu, Shuxuan Quan, Di Xu, Xiaofeng Chen, Laiyi Li, Wenchang Xu, Fanying Kong, Haisen Zhang, Pinghua Li, Bosheng Li, Gang Li
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引用次数: 0

摘要

叶角(LA)是影响玉米种植密度和产量的关键因素。然而,LA 形成的调控机制在很大程度上仍不为人所知。在这项研究中,我们对不同玉米近交系的叶舌区进行了组织学比较分析,结果表明,LA 的大小受两步调控过程的显著影响,其中包括最初的细胞伸长,随后是叶舌正面基部细胞(SC)的木质化。我们进行了大量 RNA 测序和单核 RNA 测序,生成了韧皮部区域的综合转录组图谱,并确定了下胚层细胞中富集的许多基因,这些基因可能会影响它们向 SC 的特化。此外,我们还从功能上鉴定了两个编码非典型碱性螺旋-环-螺旋(bHLH)转录因子的基因,分别是 bHLH30 及其同源物 bHLH155,它们在伸长的正面细胞中高度表达。遗传分析表明,bHLH30 和 bHLH155 能正向调节 LA 的扩展,分子实验证明它们能激活参与 SC 细胞伸长和木质化的基因转录。这些发现凸显了韧皮部正面SC在LA调控中的特殊功能,即限制韧皮部细胞的进一步延伸并增强机械强度。单核分辨率的韧带区域转录组图谱不仅加深了我们对LA调控的理解,还为现代玉米育种中优化植物结构确定了许多潜在靶标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Histological and single-nucleus transcriptome analyses reveal the specialized functions of ligular sclerenchyma cells and key regulators of leaf angle in maize.

Leaf angle (LA) is a crucial factor that affects planting density and yield in maize. However, the regulatory mechanisms underlying LA formation remain largely unknown. In this study, we performed a comparative histological analysis of the ligular region across various maize inbred lines and revealed that LA is significantly influenced by a two-step regulatory process involving initial cell elongation followed by subsequent lignification in the ligular adaxial sclerenchyma cells (SCs). Subsequently, we performed both bulk and single-nucleus RNA sequencing, generated a comprehensive transcriptomic atlas of the ligular region, and identified numerous genes enriched in the hypodermal cells that may influence their specialization into SCs. Furthermore, we functionally characterized two genes encoding atypical basic-helix-loop-helix (bHLH) transcription factors, bHLH30 and its homolog bHLH155, which are highly expressed in the elongated adaxial cells. Genetic analyses revealed that bHLH30 and bHLH155 positively regulate LA expansion, and molecular experiments demonstrated their ability to activate the transcription of genes involved in cell elongation and lignification of SCs. These findings highlight the specialized functions of ligular adaxial SCs in LA regulation by restricting further extension of ligular cells and enhancing mechanical strength. The transcriptomic atlas of the ligular region at single-nucleus resolution not only deepens our understanding of LA regulation but also enables identification of numerous potential targets for optimizing plant architecture in modern maize breeding.

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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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