S. V. Akulinichev, S. I. Glukhov, A. V. Efremenko, D. A. Kokontsev, E. A. Kuznetsova, V. V. Martynova, A. V. Feofanov, I. A. Yakovlev
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The Cellular Response to Exposure to Ionizing Radiation and Light in the Presence of a Photosensitizer
The combined use of X-ray irradiation and photodynamic therapy on a model of human lung adenocarcinoma A549 cells has been investigated. It was found that synchronous exposure to two types of radiation contributed to a stronger decrease in the survival rate of tumor cells than their sequential use or each type of radiation separately. The levels of RNA and expression of a number of genes involved in cell cycle control (p21 and DINOL), apoptosis (BAX and PUMA) and DNA repair (PARP1, OGG1, Rad51, and Lig4) were determined for various combinations of these two types of irradiations of cells. The highest level of expression under all treatments was observed for the long noncoding RNA of DINOL gene. The detected increase in the expression of the BAX and PUMA genes indicated the development of apoptosis. The results we obtained confirmed the synergism of radiation and photodynamic therapy upon their simultaneous use.
BiophysicsBiochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍：
Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
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