{"title":"不同水产养殖底质对巴布亚裸鲤免疫反应的转录组分析","authors":"Jiahua Zhang, Jie Wang, Zhaojun Gu, Xingguo Liu","doi":"10.1007/s10126-024-10324-w","DOIUrl":null,"url":null,"abstract":"<div><p>To assess the impact of different substrates in a recirculating water system on the immune response and antioxidant capacity of <i>Babylonia areolata</i>, we conducted a comparative analysis of the transcriptomes and antioxidant performance of the digestive glands in three substrate environments (sand—S group, ceramic granules—C group, and PVC breeding nest—P group). Transcriptome results revealed that the S group and P group exhibited the highest number of differentially expressed genes (DEGs), with a total of 2218 DEGs, including 928 upregulated and 1290 downregulated DEGs. The C group and P group had 1055 DEGs in common, with 316 upregulated and 739 downregulated DEGs. The C group and S group had the fewest DEGs, with 521 in total, including 303 upregulated and 218 downregulated DEGs. GO enrichment analysis showed that in the S vs P group, terms such as catalytic activity, membrane part, and cellular process were enriched with 287, 262, and 180 DEGs, respectively. In the C vs P group, binding, cellular process, and cell part were enriched with 146, 135, and 127 DEGs, respectively. In the C vs S group, catalytic activity, membrane part, and metabolic process were enriched with 90, 83, and 59 DEGs, respectively. Kegg enrichment analysis revealed significant changes in immune-related pathways in the S vs P group, including lysosome, phagosome, and leukocyte transendothelial migration, with 30, 13, and 10 enriched DEGs, respectively. In the C vs P group, phagosome, drug metabolism—other enzymes, and N-Glycan biosynthesis showed significant changes in immune-related pathways, with 9, 6, and 4 enriched DEGs, respectively. In the C vs S group, lysosome, PPAR signaling pathway, and fatty acid degradation exhibited significant changes in immune-related pathways, with 8, 4, and 3 enriched DEGs, respectively. Regarding antioxidant capacity, the S group showed significantly higher total T-AOC than the other experimental groups, while CAT, SOD, POD, and AKP were lower than in the C and P groups. The ACP level in the Sand group was not significantly different from the P group but significantly lower than the C group. In conclusion, substrate environments significantly influence the immune-related genes and key antioxidant enzyme activities in <i>B. areolata</i>.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"26 3","pages":"609 - 622"},"PeriodicalIF":2.6000,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transcriptome Analysis of Different Aquaculture Substrates on the Immune Response of Babylonia areolata\",\"authors\":\"Jiahua Zhang, Jie Wang, Zhaojun Gu, Xingguo Liu\",\"doi\":\"10.1007/s10126-024-10324-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>To assess the impact of different substrates in a recirculating water system on the immune response and antioxidant capacity of <i>Babylonia areolata</i>, we conducted a comparative analysis of the transcriptomes and antioxidant performance of the digestive glands in three substrate environments (sand—S group, ceramic granules—C group, and PVC breeding nest—P group). Transcriptome results revealed that the S group and P group exhibited the highest number of differentially expressed genes (DEGs), with a total of 2218 DEGs, including 928 upregulated and 1290 downregulated DEGs. The C group and P group had 1055 DEGs in common, with 316 upregulated and 739 downregulated DEGs. The C group and S group had the fewest DEGs, with 521 in total, including 303 upregulated and 218 downregulated DEGs. GO enrichment analysis showed that in the S vs P group, terms such as catalytic activity, membrane part, and cellular process were enriched with 287, 262, and 180 DEGs, respectively. In the C vs P group, binding, cellular process, and cell part were enriched with 146, 135, and 127 DEGs, respectively. In the C vs S group, catalytic activity, membrane part, and metabolic process were enriched with 90, 83, and 59 DEGs, respectively. Kegg enrichment analysis revealed significant changes in immune-related pathways in the S vs P group, including lysosome, phagosome, and leukocyte transendothelial migration, with 30, 13, and 10 enriched DEGs, respectively. In the C vs P group, phagosome, drug metabolism—other enzymes, and N-Glycan biosynthesis showed significant changes in immune-related pathways, with 9, 6, and 4 enriched DEGs, respectively. In the C vs S group, lysosome, PPAR signaling pathway, and fatty acid degradation exhibited significant changes in immune-related pathways, with 8, 4, and 3 enriched DEGs, respectively. Regarding antioxidant capacity, the S group showed significantly higher total T-AOC than the other experimental groups, while CAT, SOD, POD, and AKP were lower than in the C and P groups. The ACP level in the Sand group was not significantly different from the P group but significantly lower than the C group. In conclusion, substrate environments significantly influence the immune-related genes and key antioxidant enzyme activities in <i>B. areolata</i>.</p></div>\",\"PeriodicalId\":690,\"journal\":{\"name\":\"Marine Biotechnology\",\"volume\":\"26 3\",\"pages\":\"609 - 622\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-05-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Marine Biotechnology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10126-024-10324-w\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine Biotechnology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10126-024-10324-w","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
为了评估循环水系统中不同基质对巴布亚裸鲤免疫反应和抗氧化能力的影响,我们对三种基质环境(沙-S组、陶粒-C组和PVC养殖巢-P组)中消化腺的转录组和抗氧化性能进行了比较分析。转录组结果显示,S组和P组的差异表达基因(DEGs)数量最多,共有2218个DEGs,包括928个上调DEGs和1290个下调DEGs。C 组和 P 组共有 1055 个 DEGs,其中上调 DEGs 316 个,下调 DEGs 739 个。C 组和 S 组的 DEGs 最少,共有 521 个,其中上调 DEGs 303 个,下调 DEGs 218 个。GO 富集分析表明,在 S 组与 P 组中,催化活性、膜部分和细胞过程等术语分别富集了 287 个、262 个和 180 个 DEGs。在 C 组与 P 组中,结合、细胞过程和细胞部分分别富集了 146、135 和 127 个 DEGs。在 C 组与 S 组中,催化活性、膜部分和代谢过程分别富集了 90、83 和 59 个 DEGs。Kegg富集分析显示,S组与P组的免疫相关通路发生了显著变化,包括溶酶体、吞噬体和白细胞跨内皮迁移,分别富集了30、13和10个DEGs。在 C 组与 P 组中,吞噬体、药物代谢-其他酶和 N-糖生物合成在免疫相关通路中显示出显著变化,分别富集了 9、6 和 4 个 DEGs。在 C 组与 S 组中,溶酶体、PPAR 信号通路和脂肪酸降解在免疫相关通路中表现出显著变化,分别富集了 8、4 和 3 个 DEGs。在抗氧化能力方面,沙组的总 T-AOC 明显高于其他实验组,而 CAT、SOD、POD 和 AKP 则低于 C 组和 P 组。沙组的 ACP 水平与 P 组无明显差异,但明显低于 C 组。总之,底物环境会明显影响雌花蛙的免疫相关基因和关键抗氧化酶的活性。
Transcriptome Analysis of Different Aquaculture Substrates on the Immune Response of Babylonia areolata
To assess the impact of different substrates in a recirculating water system on the immune response and antioxidant capacity of Babylonia areolata, we conducted a comparative analysis of the transcriptomes and antioxidant performance of the digestive glands in three substrate environments (sand—S group, ceramic granules—C group, and PVC breeding nest—P group). Transcriptome results revealed that the S group and P group exhibited the highest number of differentially expressed genes (DEGs), with a total of 2218 DEGs, including 928 upregulated and 1290 downregulated DEGs. The C group and P group had 1055 DEGs in common, with 316 upregulated and 739 downregulated DEGs. The C group and S group had the fewest DEGs, with 521 in total, including 303 upregulated and 218 downregulated DEGs. GO enrichment analysis showed that in the S vs P group, terms such as catalytic activity, membrane part, and cellular process were enriched with 287, 262, and 180 DEGs, respectively. In the C vs P group, binding, cellular process, and cell part were enriched with 146, 135, and 127 DEGs, respectively. In the C vs S group, catalytic activity, membrane part, and metabolic process were enriched with 90, 83, and 59 DEGs, respectively. Kegg enrichment analysis revealed significant changes in immune-related pathways in the S vs P group, including lysosome, phagosome, and leukocyte transendothelial migration, with 30, 13, and 10 enriched DEGs, respectively. In the C vs P group, phagosome, drug metabolism—other enzymes, and N-Glycan biosynthesis showed significant changes in immune-related pathways, with 9, 6, and 4 enriched DEGs, respectively. In the C vs S group, lysosome, PPAR signaling pathway, and fatty acid degradation exhibited significant changes in immune-related pathways, with 8, 4, and 3 enriched DEGs, respectively. Regarding antioxidant capacity, the S group showed significantly higher total T-AOC than the other experimental groups, while CAT, SOD, POD, and AKP were lower than in the C and P groups. The ACP level in the Sand group was not significantly different from the P group but significantly lower than the C group. In conclusion, substrate environments significantly influence the immune-related genes and key antioxidant enzyme activities in B. areolata.
期刊介绍:
Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.