脱细胞血管移植物作为血液透析通道在体内的适应过程。

IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Regenerative Biomaterials Pub Date : 2024-03-21 eCollection Date: 2024-01-01 DOI:10.1093/rb/rbae029
Tun Wang, Peng Lu, Zicheng Wan, Zhenyu He, Siyuan Cheng, Yang Zhou, Sheng Liao, Mo Wang, Tianjian Wang, Chang Shu
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引用次数: 0

摘要

动静脉移植物(AVG)已成为众多患者构建血液透析通路的首选。临床试验表明,与聚合物材料相比,脱细胞血管移植物具有更高的通畅性和良好的生物相容性;但它仍然面临着内膜增生和管腔扩张等挑战。由于缺乏合适的动物模型,我们无法从分子水平描述和解释脱细胞血管移植的上述病理现象和体内适应过程。在本研究中,我们首先收集了使用异体脱细胞血管移植物构建透析通路的患者的临床样本,并评估了他们移植后 5 年的组织学特征和免疫细胞浸润状况。手术前,我们使用无创超声评估了脱细胞血管移植的通畅性和内膜增生情况。随后,为了研究脱细胞血管移植物在动物模型中的体内适应性,我们尝试在小动物模型中使用脱细胞血管移植物构建 AVG 模型。我们采用物理-化学-生物方法对大鼠颈动脉进行脱细胞处理,组织学评估结果表明,在保留细胞外基质成分(如弹性纤维和胶原纤维)的同时,成功去除了细胞和抗原成分。基于这些结果,我们设计并构建了首个异体脱细胞大鼠颈动脉 AVG 模型,该模型显示出良好的通畅性,并与临床特征非常相似。利用该动物模型,我们初步描述了脱细胞血管移植物在不同时间点的组织学特征和部分免疫细胞浸润情况,包括植入后第 7 天、第 21 天、第 42 天和一年。这些发现为进一步研究脱细胞血管移植物在小动物模型中的体内适应过程奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Adaptation process of decellularized vascular grafts as hemodialysis access in vivo.

Arteriovenous grafts (AVGs) have emerged as the preferred option for constructing hemodialysis access in numerous patients. Clinical trials have demonstrated that decellularized vascular graft exhibits superior patency and excellent biocompatibility compared to polymer materials; however, it still faces challenges such as intimal hyperplasia and luminal dilation. The absence of suitable animal models hinders our ability to describe and explain the pathological phenomena above and in vivo adaptation process of decellularized vascular graft at the molecular level. In this study, we first collected clinical samples from patients who underwent the construction of dialysis access using allogeneic decellularized vascular graft, and evaluated their histological features and immune cell infiltration status 5 years post-transplantation. Prior to the surgery, we assessed the patency and intimal hyperplasia of the decellularized vascular graft using non-invasive ultrasound. Subsequently, in order to investigate the in vivo adaptation of decellularized vascular grafts in an animal model, we attempted to construct an AVG model using decellularized vascular grafts in a small animal model. We employed a physical-chemical-biological approach to decellularize the rat carotid artery, and histological evaluation demonstrated the successful removal of cellular and antigenic components while preserving extracellular matrix constituents such as elastic fibers and collagen fibers. Based on these results, we designed and constructed the first allogeneic decellularized rat carotid artery AVG model, which exhibited excellent patency and closely resembled clinical characteristics. Using this animal model, we provided a preliminary description of the histological features and partial immune cell infiltration in decellularized vascular grafts at various time points, including Day 7, Day 21, Day 42, and up to one-year post-implantation. These findings establish a foundation for further investigation into the in vivo adaptation process of decellularized vascular grafts in small animal model.

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来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
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