{"title":"一岁半食物过敏儿童和健康儿童的肠道微生物群。","authors":"","doi":"10.1016/j.alit.2024.03.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Intestinal bacteria may play a role in the development of food allergies. This study aimed to analyze and compare the gut microbiota of food-allergic children with that of healthy children of the same age.</p></div><div><h3>Methods</h3><p>Stool samples were collected from one-and-a-half-year-old food-allergic (FA group, n = 29) and healthy controls (HC group, n = 19). A questionnaire was provided to examine the children's birth, dietary, medical, and social histories. The gut microbiota was profiled by 16S rRNA sequencing. Differences in taxonomic composition were assessed using linear discriminant analysis effect size (LEfSe), and microbial functional profiles were predicted with Tax4Fun2.</p></div><div><h3>Results</h3><p>No significant difference in the alpha diversity index between the two groups; however, a negative correlation was observed between the Shannon diversity index and the relative abundance of <em>Bacteroides</em>. A significant difference was observed in beta diversity (permutational multivariate analysis of variance) in the bacterial composition between the FA and HC groups (<em>P</em> < 0.05). The FA group had a higher abundance of <em>Escherichia</em> and <em>Anaeromassilibacillus</em> and a lower abundance of <em>Bacteroides</em>, <em>Oscillibacter</em>, <em>Ruminococcus, Hungateiclostridium</em> and <em>Anaerotaenia</em> than the HC group (LEfSe: linear discriminant analysis score >2). The FA group showed a predicted increase in the expression levels of genes associated with intestinal pathogenicity compared with that in the HC group.</p></div><div><h3>Conclusions</h3><p>The gut microbiota of food-allergic children has a higher abundance of bacteria involved in intestinal inflammation and a lower abundance of bacteria involved in immune tolerance than that of healthy children. This dysbiosis may also be associated with food allergies.</p></div>","PeriodicalId":48861,"journal":{"name":"Allergology International","volume":"73 4","pages":"Pages 550-555"},"PeriodicalIF":6.2000,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S132389302400042X/pdfft?md5=df0126a6bc0acb112048fcdd3d8f1a04&pid=1-s2.0-S132389302400042X-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Gut microbiota of one-and-a-half-year-old food-allergic and healthy children\",\"authors\":\"\",\"doi\":\"10.1016/j.alit.2024.03.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Intestinal bacteria may play a role in the development of food allergies. This study aimed to analyze and compare the gut microbiota of food-allergic children with that of healthy children of the same age.</p></div><div><h3>Methods</h3><p>Stool samples were collected from one-and-a-half-year-old food-allergic (FA group, n = 29) and healthy controls (HC group, n = 19). A questionnaire was provided to examine the children's birth, dietary, medical, and social histories. The gut microbiota was profiled by 16S rRNA sequencing. Differences in taxonomic composition were assessed using linear discriminant analysis effect size (LEfSe), and microbial functional profiles were predicted with Tax4Fun2.</p></div><div><h3>Results</h3><p>No significant difference in the alpha diversity index between the two groups; however, a negative correlation was observed between the Shannon diversity index and the relative abundance of <em>Bacteroides</em>. A significant difference was observed in beta diversity (permutational multivariate analysis of variance) in the bacterial composition between the FA and HC groups (<em>P</em> < 0.05). The FA group had a higher abundance of <em>Escherichia</em> and <em>Anaeromassilibacillus</em> and a lower abundance of <em>Bacteroides</em>, <em>Oscillibacter</em>, <em>Ruminococcus, Hungateiclostridium</em> and <em>Anaerotaenia</em> than the HC group (LEfSe: linear discriminant analysis score >2). The FA group showed a predicted increase in the expression levels of genes associated with intestinal pathogenicity compared with that in the HC group.</p></div><div><h3>Conclusions</h3><p>The gut microbiota of food-allergic children has a higher abundance of bacteria involved in intestinal inflammation and a lower abundance of bacteria involved in immune tolerance than that of healthy children. This dysbiosis may also be associated with food allergies.</p></div>\",\"PeriodicalId\":48861,\"journal\":{\"name\":\"Allergology International\",\"volume\":\"73 4\",\"pages\":\"Pages 550-555\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2024-04-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S132389302400042X/pdfft?md5=df0126a6bc0acb112048fcdd3d8f1a04&pid=1-s2.0-S132389302400042X-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Allergology International\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S132389302400042X\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ALLERGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Allergology International","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S132389302400042X","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ALLERGY","Score":null,"Total":0}
引用次数: 0
摘要
背景:肠道细菌可能在食物过敏的发病过程中起作用。本研究旨在分析和比较食物过敏儿童与同龄健康儿童的肠道微生物群:方法:收集一岁半食物过敏儿童(FA 组,29 人)和健康对照组(HC 组,19 人)的粪便样本。通过问卷调查了解儿童的出生史、饮食史、病史和社会史。通过 16S rRNA 测序分析了肠道微生物群。使用线性判别分析效应大小(LEfSe)评估了分类组成的差异,并使用 Tax4Fun2 预测了微生物功能特征:两组之间的阿尔法多样性指数无明显差异;但香农多样性指数与乳杆菌的相对丰度之间呈负相关。FA 组和 HC 组细菌组成的贝塔多样性(包络多变量方差分析)存在明显差异(P 2)。与 HC 组相比,FA 组与肠道致病性相关基因的表达水平预计会增加:结论:与健康儿童相比,食物过敏儿童肠道微生物群中参与肠道炎症的细菌数量较多,而参与免疫耐受的细菌数量较少。这种菌群失调也可能与食物过敏有关。
Gut microbiota of one-and-a-half-year-old food-allergic and healthy children
Background
Intestinal bacteria may play a role in the development of food allergies. This study aimed to analyze and compare the gut microbiota of food-allergic children with that of healthy children of the same age.
Methods
Stool samples were collected from one-and-a-half-year-old food-allergic (FA group, n = 29) and healthy controls (HC group, n = 19). A questionnaire was provided to examine the children's birth, dietary, medical, and social histories. The gut microbiota was profiled by 16S rRNA sequencing. Differences in taxonomic composition were assessed using linear discriminant analysis effect size (LEfSe), and microbial functional profiles were predicted with Tax4Fun2.
Results
No significant difference in the alpha diversity index between the two groups; however, a negative correlation was observed between the Shannon diversity index and the relative abundance of Bacteroides. A significant difference was observed in beta diversity (permutational multivariate analysis of variance) in the bacterial composition between the FA and HC groups (P < 0.05). The FA group had a higher abundance of Escherichia and Anaeromassilibacillus and a lower abundance of Bacteroides, Oscillibacter, Ruminococcus, Hungateiclostridium and Anaerotaenia than the HC group (LEfSe: linear discriminant analysis score >2). The FA group showed a predicted increase in the expression levels of genes associated with intestinal pathogenicity compared with that in the HC group.
Conclusions
The gut microbiota of food-allergic children has a higher abundance of bacteria involved in intestinal inflammation and a lower abundance of bacteria involved in immune tolerance than that of healthy children. This dysbiosis may also be associated with food allergies.
期刊介绍:
Allergology International is the official journal of the Japanese Society of Allergology and publishes original papers dealing with the etiology, diagnosis and treatment of allergic and related diseases. Papers may include the study of methods of controlling allergic reactions, human and animal models of hypersensitivity and other aspects of basic and applied clinical allergy in its broadest sense.
The Journal aims to encourage the international exchange of results and encourages authors from all countries to submit papers in the following three categories: Original Articles, Review Articles, and Letters to the Editor.