培养阳性真菌性角膜炎的微生物特征

IF 2 4区 医学 Q2 OPHTHALMOLOGY
Ipsita Muni, Himanshu Sekhar Behera, Srikant Kumar Sahu, Smruti Rekha Priyadarshini, Sujata Das
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引用次数: 0

摘要

目的:研究在印度东部一家三级眼科医疗中心就诊的培养阳性真菌性角膜炎病例的微生物学特征:回顾性审查了 2020 年 1 月至 2021 年 12 月期间在布巴内斯瓦尔市 L V Prasad 眼科研究所就诊的所有培养阳性真菌性角膜炎患者的微生物学记录。收集的数据包括真菌或混合感染培养阳性的涂片结果、分离出的菌种以及菌种在每种培养基中生长所需的时间:结果:真菌性角膜炎占所有培养阳性微生物性角膜炎的 36%,而混合感染(真菌和其他生物)占 8.5%。最常见的真菌是镰刀菌属(25.8%)。与真菌混合感染最常见的细菌是葡萄球菌属(54.8%)。氢氧化钾+氟化钙白染色法检测真菌菌丝的阳性率为 89.0%,革兰氏染色法的阳性率为 76.1%。培养阳性的真菌性角膜炎病例最常在钾葡萄糖琼脂上生长(77.6%)。在培养阳性的混合感染中也观察到类似的模式(沙保鲁葡萄糖琼脂 [SDA]:84%)。混合感染中细菌生长最频繁的是巯基乙酸肉汤(54.7%)。在血琼脂(BA)和巧克力琼脂(CA)中,真菌显著生长所需的时间最短(真菌性角膜炎和混合感染分别为 2.2 天/2.3 天和 1.8 天/2 天)。丝状透明真菌显著生长所需的时间最短(2.8 天),而酵母菌所需的时间最长(5 天):本研究强调了结合使用固体和液体培养基(尤其是马铃薯葡萄糖琼脂(PDA)/SDA 和 CA)对真菌性角膜炎和可能的细菌合并感染做出明确诊断的重要性,细菌合并感染在真菌性角膜炎病例中占很大比例。在资源匮乏的实验室中,可将两种培养基(SDA 或 PDA)与 BA 一起培养,以检测混合感染。在没有培养基的情况下,检查角膜样本的染色涂片是快速诊断真菌性角膜炎的一种廉价方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbiological Profile of Culture-Positive Fungal Keratitis.

Purpose: To examine the microbiological profile of cases of culture-positive fungal keratitis presenting to a tertiary eye care center in eastern India.

Methods: Microbiology records of all culture-positive microbial keratitis patients presenting to L V Prasad Eye Institute, Bhubaneswar, between January 2020 and December 2021, were retrospectively reviewed. Collected data included smear results of culture-positive fungal or mixed infections, the species isolated, and the time taken for organisms to grow in each media.

Results: Fungal keratitis formed 36% of all culture-positive microbial keratitis, whereas mixed infections (fungi and other organisms) formed 8.5%. The most common fungal species isolated was Fusarium spp. (25.8%). The most common bacteria involved in mixed infection with fungi was Staphylococcus spp. (54.8%). The positivity of potassium hydroxide+calcofluor white stain in detecting fungal filaments was 89.0% and that of Gram stain was 76.1%. Culture-positive cases of fungal keratitis showed most frequent growth on potato-dextrose agar (77.6%). A similar pattern was observed in culture-positive mixed infections (Sabouraud dextrose agar [SDA]: 84%). Most frequent growth of bacteria in mixed infections was seen in thioglycolate broth (54.7%). The shortest time to achieve significant fungal growth was observed in blood agar (BA) and chocolate agar (CA) (2.2/2.3 days, and 1.8/2 days for fungal keratitis and mixed infections, respectively). Filamentous hyaline fungi took the shortest time to achieve significant growth (2.8 days), whereas yeast forms took the longest (5 days).

Conclusion: This study highlights the importance of combined use of both solid and liquid culture media, especially potato dextrose agar (PDA)/SDA and CA, to arrive at a definitive diagnosis of fungal keratitis and possible bacterial co-infection, which forms a significant proportion of cases with fungal keratitis. In resource-poor laboratories, two culture media, either SDA or PDA, along with BA, may be plated to detect mixed infections. Examination of stained smears of corneal samples provides an inexpensive method of rapid diagnosis of fungal keratitis when culture media is not available.

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来源期刊
CiteScore
4.50
自引率
4.30%
发文量
150
审稿时长
6-12 weeks
期刊介绍: Eye & Contact Lens: Science and Clinical Practice is the official journal of the Contact Lens Association of Ophthalmologists (CLAO), an international educational association for anterior segment research and clinical practice of interest to ophthalmologists, optometrists, and other vision care providers and researchers. Focusing especially on contact lenses, it also covers dry eye disease, MGD, infections, toxicity of drops and contact lens care solutions, topography, cornea surgery and post-operative care, optics, refractive surgery and corneal stability (eg, UV cross-linking). Peer-reviewed and published six times annually, it is a highly respected scientific journal in its field.
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