为下一代纤维素酶生产定制真菌,特别是 CRISPR/CAS 系统。

Subhadeep Mondal, Suman Kumar Halder, Keshab Chandra Mondal
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引用次数: 0

摘要

纤维素是地球上最丰富的生物聚合物来源,而真菌是通过合成纤维素酶降解纤维素生物质的最有效、最普遍的生物。在构建新型真菌菌株以提高纤维素酶产量、改善所需性状的过程中,通过遗传操作进行定制发挥了重要作用。然而,传统的真菌遗传操作方法耗时且繁琐。随着几种与工业相关的丝状真菌全基因组序列的出现,CRISPR-CAS(簇状规则间隔短回文重复序列/CRISPR相关蛋白)技术成为熟练开发丝状真菌操纵菌株的焦点。本综述概述了纤维素酶的作用模式、纤维素酶表达的转录水平调控、利用 CRISPR-CAS 技术开发改良真菌菌株以提高纤维素酶产量的各种传统遗传操作策略,以及这一研究领域的未来趋势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tailoring in fungi for next generation cellulase production with special reference to CRISPR/CAS system.

Cellulose is the utmost plenteous source of biopolymer in our earth, and fungi are the most efficient and ubiquitous organism in degrading the cellulosic biomass by synthesizing cellulases. Tailoring through genetic manipulation has played a substantial role in constructing novel fungal strains towards improved cellulase production of desired traits. However, the traditional methods of genetic manipulation of fungi are time-consuming and tedious. With the availability of the full-genome sequences of several industrially relevant filamentous fungi, CRISPR-CAS (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein) technology has come into the focus for the proficient development of manipulated strains of filamentous fungi. This review summarizes the mode of action of cellulases, transcription level regulation for cellulase expression, various traditional strategies of genetic manipulation with CRISPR-CAS technology to develop modified fungal strains for a preferred level of cellulase production, and the futuristic trend in this arena of research.

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