在过氧化氢和脱氧雪腐镰刀菌醇的挑战下,副流感杆菌 809 和枯草芽孢杆菌 810 支持体外肠道完整性。

IF 1.3 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Translational Animal Science Pub Date : 2024-04-13 eCollection Date: 2024-01-01 DOI:10.1093/tas/txae061
Erik J Boll, Giuseppe Copani, Bruno I Cappellozza
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引用次数: 0

摘要

我们设计并进行了两项体外实验,在两种不同的挑战模型下使用经上皮电阻(TEER)测定法评估两种益生菌对肠道屏障完整性的影响。在实验 1 中,肠上皮细胞接受或不接受(CON)副链霉菌 809(BLI)或枯草杆菌 810(BSU),菌落形成单位(CFU)为 1 × 108 个/透孔。处理(CON、BLI 或 BSU)两小时后,单独(HYP)或与每种芽孢杆菌属(HYP + BLI 或 HYP + BSU)一起加入 5 mM 活性氧过氧化氢,模拟粘膜氧化应激。在实验 2 中,细胞被分配到与实验 1 相同的处理(CON、BLI 和 BSU),或霉菌毒素脱氧雪腐镰刀菌醇(DON),后者被单独添加或与 BLI 或 BSU 结合添加,从而产生另外两种处理(DON + BLI 和 DON + BSU)。挑战后 14 小时测量跨皮层电阻。在实验 1 中,观察到 TEER 的处理 × 小时交互作用(P P AUC),而通过单层细胞转运的糖量增加(P P P Bacillus spp.减轻了 DON 单独造成的损害,通过单层细胞转运的糖的比例在 CON 和 DON + BLI(P = 0.14)以及 DON + BLI 和 DON + BSU(P = 0.62)之间没有差异。总之,两种芽孢杆菌属菌株(B. paralicheniformis 809 和 B. subtilis 810)都能抵消过氧化氢和脱氧雪腐镰刀菌醇这两种挑战剂对肠道屏障完整性的破坏作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Bacillus paralicheniformis 809 and Bacillus subtilis 810 support in vitro intestinal integrity under hydrogen peroxide and deoxynivalenol challenges.

We designed and conducted two in vitro experiments to evaluate the effects of two Bacillus spp. probiotics on gut barrier integrity using the transepithelial electrical resistance (TEER) assay under two different challenge models. In Exp. 1, intestinal epithelial cells received or not (CON) B. paralicheniformis 809 (BLI) or B. subtilis 810 (BSU) at a rate of 1 × 108 colony forming units (CFU)/transwell. Two hours after treatment application (CON, BLI, or BSU), 5 mM of the reactive oxygen species hydrogen peroxide, mimicking mucosal oxidative stress, was added alone (HYP) or with each of the Bacillus spp. (HYP + BLI or HYP + BSU). In Exp. 2, cells were assigned to the same treatments as in Exp. 1 (CON, BLI, and BSU), or mycotoxin deoxynivalenol (DON), which was added alone or in combination with BLI or BSU, resulting in another two treatments (DON + BLI and DON + BSU). Transepithelial electrical resistance was measured for 14 h postchallenge. In Exp. 1, a treatment × hour interaction was observed for TEER (P < 0.0001). Adding BLI and BSU resulted in greater TEER values vs. CON for most of the experimental period (P < 0.02), whereas HYP reduced mean TEER and area under the curve (AUC), while increasing the amount of sugar that translocated through the monolayer cells (P < 0.001). A treatment × hour interaction was also observed in Exp. 2 (P < 0.0001), as DON led to an immediate and acute drop in TEER that lasted until the end of the experimental period (P < 0.0001). Both BLI and BSU alleviated the DON-induced damaging effects on the integrity of intestinal epithelial cells, whereas both Bacillus spp. alleviated the damage caused by DON alone and the proportion of sugar that translocated through the monolayer cells was not different between CON and DON + BLI (P = 0.14) and DON + BLI and DON + BSU (P = 0.62). In summary, both Bacillus spp. strains (B. paralicheniformis 809 and B. subtilis 810) were able to counteract the damaging effects of the challenge agents, hydrogen peroxide and deoxynivalenol, on gut barrier integrity.

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来源期刊
Translational Animal Science
Translational Animal Science Veterinary-Veterinary (all)
CiteScore
2.80
自引率
15.40%
发文量
149
审稿时长
8 weeks
期刊介绍: Translational Animal Science (TAS) is the first open access-open review animal science journal, encompassing a broad scope of research topics in animal science. TAS focuses on translating basic science to innovation, and validation of these innovations by various segments of the allied animal industry. Readers of TAS will typically represent education, industry, and government, including research, teaching, administration, extension, management, quality assurance, product development, and technical services. Those interested in TAS typically include animal breeders, economists, embryologists, engineers, food scientists, geneticists, microbiologists, nutritionists, veterinarians, physiologists, processors, public health professionals, and others with an interest in animal production and applied aspects of animal sciences.
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