开发基于多物种蛋白 A 的新型酶联免疫吸附试验，用于家猪戊型肝炎感染的血清诊断和验证

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2024-08-01 Epub Date: 2024-05-06 DOI:10.1089/fpd.2023.0179

Maria Gabriela Oliveira da Paz, Matheus Filgueira Bezerra, Andrei Félix Mendes, Adalucia da Silva, Gustavo Barbosa de Lima, Laura Helena Vega Gonzales Gil, Lindomar José Pena, George Tadeu Nunes Diniz, Jan Felix Drexler, Reinaldo Barreto Oriá, Edmilson Ferreira de Oliveira-Filho, Christian Robson de Souza Reis

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引用次数: 0

摘要

全球约有 2000 万人感染戊型肝炎病毒（HEV），该病毒可引起自限性急性肝病，并可演变为慢性病程。HEV-3、HEV-4 和 HEV-7 基因型是人畜共患疾病，通过食用生肉或未煮熟的肉类传染给人类。在此，我们开发了一种基于重组 HEV-3 外壳的间接 ELISA，并对巴西东北部的家猪进行了血清流行率研究。我们的内部 ELISA 最初通过 79 份血清进行了验证，这 79 份血清的特点是与两种不同的商业 ELISA 试剂盒的结果一致。我们的酶联免疫吸附测定法具有极高的灵敏度（94%）和特异性（100%），曲线下面积为 0.99。进一步的测试（包括 212 份猪血清）显示，血清流行率为 57.5%（95% 置信区间，50.6-64.3%）。我们的研究结果表明，新型酶联免疫吸附试验能准确检测家猪体内的特异性抗 HEV 抗体，应在人类和其他哺乳动物中进一步验证。

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Development of a New Multispecies Protein A-Based ELISA for Serodiagnosis of Hepatitis E Infection with Validation in Domestic Swine.

Hepatitis E virus (HEV) infects roughly 20 million people worldwide, causing self-limiting acute hepatic disease that can evolve into a chronic course. HEV-3, HEV-4, and HEV-7 genotypes are zoonotic and transmitted to humans by consuming raw or undercooked meat. Here, we developed an indirect ELISA based on the recombinant HEV-3 capsid and performed a seroprevalence study on domestic swine in northeastern Brazil. Our in-house ELISA was initially validated using a subset of 79 sera characterized by concordant results for two distinct commercial ELISA kits. Our ELISA exhibited excellent sensitivity (94%) and specificity (100%), with an area under the curve of 0.99 Further testing, including 212 swine sera, revealed a seroprevalence of 57.5% (95% confidence interval, 50.6-64.3%). Our findings indicate that the novel ELISA test could accurately detect specific anti-HEV antibodies in domestic pigs and should be further validated in humans and other mammals.

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.