有术后谵妄行为的老年小鼠唾液和不同脑区的代谢组学分析

Xiao Liu, Ying Cao, Xiao Wan Lin, Dan Yang Gao, Hui Hui Miao, Tian Zuo Li
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引用次数: 0

摘要

目的:随着全球人口的老龄化,术后谵妄(POD)已成为一项严峻的挑战,其后果严重,发病率也在增加。然而,其潜在机制尚不清楚。我们的研究旨在探讨有术后谵妄行为的老年小鼠三个特定脑区和唾液中代谢物的变化,并确定潜在的非侵入性生物标志物:18个月大的雄性C57/BL6小鼠被随机分配到麻醉/手术组或对照组。在手术前 24 小时、手术后 6、9 和 24 小时进行行为测试。测量海马中补体C3(C3)和S100钙结合蛋白B蛋白(S100beta)的水平,并对唾液、海马、皮层和杏仁核样本进行代谢组学分析:结果:在唾液、海马、大脑皮层和杏仁核中分别检测到 43、33、38 和 14 种不同的代谢物。"丙酮酸"、"α-亚麻酸 "和 "2-油酰基-1-棕榈酰-sn-甘油-3-磷酸胆碱 "富集在一个共同的途径中,可能是潜在的 POD 非侵入性生物标志物。在三个脑区观察到了共同的变化,1-甲基组氨酸上调,D-谷氨酰胺下调:结论:能量代谢障碍、氧化应激和神经递质失调与 POD 的发病有关。识别唾液代谢物生物标志物水平的变化有助于开发 POD 的无创诊断方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Metabolomic Analysis in Saliva and Different Brain Regions of Older Mice with Postoperative Delirium Behaviors.

Objective: Postoperative delirium (POD) has become a critical challenge with severe consequences and increased incidences as the global population ages. However, the underlying mechanism is yet unknown. Our study aimed to explore the changes in metabolites in three specific brain regions and saliva of older mice with postoperative delirium behavior and to identify potential non-invasive biomarkers.

Methods: Eighteen-month-old male C57/BL6 mice were randomly assigned to the anesthesia/surgery or control group. Behavioral tests were conducted 24 h before surgery and 6, 9, and 24 h after surgery. Complement C3 (C3) and S100 calcium-binding protein B protein (S100beta) levels were measured in the hippocampus, and a metabolomics analysis was performed on saliva, hippocampus, cortex, and amygdala samples.

Results: In total, 43, 33, 38, and 14 differential metabolites were detected in the saliva, hippocampus, cortex, and amygdala, respectively. "Pyruvate" "alpha-linolenic acid" and "2-oleoyl-1-palmitoy-sn-glycero-3-phosphocholine" are enriched in one common pathway and may be potential non-invasive biomarkers for POD. Common changes were observed in the three brain regions, with the upregulation of 1-methylhistidine and downregulation of D-glutamine.

Conclusion: Dysfunctions in energy metabolism, oxidative stress, and neurotransmitter dysregulation are implicated in the development of POD. The identification of changes in the level of salivary metabolite biomarkers could aid in the development of noninvasive diagnostic methods for POD.

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